NRAP (NM_001261463) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	N-RAP
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332790 representing NM_001261463. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAATGTGCAGCCCTGTTCTAGGTGTGGGTATGGGGTTTATCCTGCCGAGAAGATCAGCTGTATAGAT CAGATATGGCATAAAGCCTGTTTTCACTGTGAAGTTTGCAAGATGATGCTGTCTGTTAATAACTTTGTG AGTCACCAGAAAAAGCCGTACTGTCACGCCCATAACCCTAAGAACAACACTTTCACCAGTGTCTATCAC ACTCCATTAAATCTAAATGTGAGGACATTTCCAGAGGCCATCAGTGGGATCCATGACCAAGAAGATGGT GAACAGTGTAAATCAGTTTTTCACTGGGACATGAAATCCAAGGATAAGGAAGGTGCACCTAACAGGCAG CCACTGGCAAATGAGAGAGCCTATTGGACTGGATATGGGGAAGGGAATGCTTGGTGCCCAGGAGCTCTG CCAGACCCCGAAATTGTAAGGATGGTTGAGGCTCGAAAGTCTCTTGGTGAGGAATATACAGAAGACTAT GAGCAACCCAGGGGCAAGGGGAGCTTTCCAGCCATGATCACACCTGCTTATCAAAGGGCCAAGAAAGCC AACCAGCTGGCCAGCCAAGTGGAGTATAAGAGAGGGCATGATGAACGCATCTCCAGGTTCTCCACGGTG GTGGATACTCCTGAGCTGCTACGGAGCAAGGCTGGGGCACAGCTTCAAAGTGATGTGAGATACACAGAG GACTATGAACAACAAAGAGGGAAAGGCAGTTTCCCTGCGATGATCACACCCGCCTATCAGATAGCCAAA AGAGCCAATGAGCTGGCAAGTGATGTGAGGTACCATCAACAATATCAAAAAGAAATGAGGGGAATGGCT GGTCCAGCCATTGGAGCTGAGGGCATCTTGACAAGGGAATGTGCAGACCAATATGGCCAGGGTTACCCG GAGGAGTATGAGGAGCACAGGGGAAAGGGCAGCTTCCCAGCTATGATCACTCCAGCATATCAGAACGCC AAGAAAGCTCACGAACTCGCTAGTGACATAAAATACAGGCAGGACTTCAATAAGATGAAAGGCGCTGCA CATTATCACTCGCTTCCAGCTCAAGACAACTTGGTTCTCAAACAGGCTCAGAGCGTAAACAAACTCGTG AGTGAGGTGGAGTATAAGAAGGATCTGGAAAGTAGTAGAGGTCACAGTATCAACTACTGTGAAACACCT CAATTCAGGAACGTGAGCAAGATCTCAAAATTTACCAGTGATAATAAATATAAAGAAAACTACCAGAAC CACATGAGAGGCCGCTATGAAGGAGTTGGTATGGACAGACGCACTCTGCATGCTATGAAAGTTGGCAGC CTGGCAAGCAACGTTGCCTACAAAGCTGATTATAAACATGATATTGTCGACTACAACTACCCAGCCACT CTCACGCCTTCCTATCAAACAGCTATGAAACTGGTGCCCTTGAAAGATGCCAATTATAGGCAGAGCATC GACAAGTTGAAGTACAGCTCGGTGACTGACACCCCACAGATTGTTCAAGCCAAAATCAATGCCCAGCAG CTGAGTCATGTGAATTACCGTGCTGACTATGAGAAAAATAAGTTGAATTACACATTGCCCCAGGATGTT CCTCAGCTGGTGAAGGCCAAAACCAATGCCAAACTCTTCAGTGAGGTTAAGTATAAAGAAGGCTGGGAG AAGACAAAGGGGAAAGGATTTGAGATGAAGCTGGATGCCATGTCTCTGCTGGCCGCCAAAGCCTCTGGG GAGCTTGCTAGCAATATTAAATATAAAGAAGAATATGAAAAGACAAAAGGCAAAGCCATGGGAACAGCC GACTCTAGGCTTCTGCACTCCCTGCAGATTGCTAAGATGAGCAGTGAGGTTGAATATAAGAAAGGCTTT GAAGAGAGTAAGACCCGGTTTCACCTGCCCATGGATATGGTAAACATCAGGCATGCTAAGAAGGCCCAA ACTCTCGCCAGTGACCTGGACTACAGGAAGAAACTGCATGAATACACTGTGCTGCCTGAAGATATGAAG ACTCAGTGGGCCAAGAAGGCCTATGGGCTCCAGAGCGAGCTGCAGTACAAGGCTGACCTGGCATGGATG AAGGGAGTTGGGTGGCTGACAGAGGGGAGTCTCAACTTGGAACAAGCCAAGAAGGCTGGACAGCTGGTC AGCGAGAAAAACTACCGGCAGAGGGTCGATGAGCTGAAGTTCACCAGCGTGACCGACAGCTCCCAGATG GAGCACGCCAAGAAGAGCCAGGAGCTACAGAGCGGGGTGGCCTACAAGGCAGGAAATGAGCAGTCTGTC CATCAGTATACCATCAGCAAAGACGAGCCTCTCTTCCTGCAGGCCCGAGCCAATGCTGCAAATCTCAGC GAGAAACTGTATAAGAGCAGCTGGGAAAACCAGAAAGCAAAAGGGTTTGAGCTGCGTCTTGATTCCTTG ACCTTCCTGGCAGCCAAAGCCAAGAGGGACCTGGCTAGCGAGGTGAAGTACAAGGAGGATTATGAGAGA TCCAGAGGGAAGCTCATTGGGGCAAAAGATGTACAGGGAGATTCGCAAATGAGCCACTCACTGCAAATG TCCAAGCTGCAGAGTGAGCTGGAGTACAAGAAGGGATTCGAGGACACCAAATCCCAATGCCACGTCTCC CTGGACATGGTCCACCTCGTGCATGCCCGCAAAGCTCAGCATTTAGCCACAGACGTAGGCTACAAGACA GCGGAACATCACTTTACGGCTTTGCCCACAGACATGAAGGTGGAATGGGCCAAGAAGGCTTATGGCTTA CAGAGTGATAACCAATACAGGGCAGATGTGAAGTGGATGAAAGGCATGGGCTGGGTCGCCACCGGGTCA TTAAATGTGGAGCAGGCGAAGAAGGCAGGAGAACTCATTAGCGAGAAGAAGTACCGTCAGCATCCAGAT GCTTTGAAGTTTACCAGTATTAAAGACACTCCGGAGATGGTCCAGGCCAGAATTAGTTATACCCAAGCA GTGGATAGATTATACAGGGAACAAGGAGAAAACATAAAGCATCATTACACACCGACTGCTGACCTCCCT GAAGTCCTGCTGGCCAAGCTGAATGCCATGAATATCAGTGAGACGCGTTATAAGGAATCCTGGAGCAAA CTTCGAGATGGTGGCTATAAACTGAGGTTGGATGCCCTTCCATTCCAAGCAGCAAAGGCTTCTGGTGAA ATCATAAGTGATTACAAATACAAAGAGGCATTTGAGAAAATGAAAGGACAGATGCTTGGTTCCCGGAGC TTGGAAGATGATATCAGCCTTGCACATTCAGTGTATGCGACCTCACTGCAGAGTGATGTGAATTACAAG AAAGGCTTTGAACACTCAAAGGCGCAGTTCCATCTGCCGTTGGACATGGCCGCCCTGGTGCATGCCAAG AAGGCTCAGACCCTGGCCAGCAATCAGGACTACAAACATCCACTGCCCCAGTACACTTCCTTGGCAGAA GACCTGAGGCTGAGCTGTGCCAAGAAAGCTCACAAATTGCAGAGTGAGAATTTGTACCGGTCAGACCTG AACTTTATGCGAGGTGTTGCATGTGTCATTCCAGGCACGTTAGAGATTGAAGGGAGGAAGAAAGCATCG GAACTCATCAGTGAGAGTAAATATCGGCAGCATCCCCACTCATTCAAGTACACAGCTGTGACAGACACT CCCAACCTCCTTCATGCGAAATTCAGCAACCAGATAACGAATGAGCGCCTCTATAAAGCAGCTGGAGAG GATGCAAGACACGAGTATACAATGACCCTGGGTCTGCCCGAGTTCATCCGAGCAAAAACGAATGCAGCC AACCTGAGTGACGCAAGATACAAAGAGTCCTGGCGTAATCTTCGTGCTCAAGGCTACAAGCTGACAATA GAAGCGCTCCCCTTCCAGGCTGCCCGGGCCTCTGGAGATATAGCCAGTGATTTTCTCTACAGACATGAC TTTGTGAAGGAGCGAGGGAAACTCATAGGGCCCCAGAGTGTAAGAGACGACCCCCGGATCCAGCACTGC CGGCGCATGGGCCAGCTGCAGAGCGAGCTTCAGTACAGGAGGGGGGCGACCAGCAGCCAAGCCCAGTTC CATCTGCCCATGGACATGGTGCACCTGGTCCATGCCAAGAATGCCCAGGCTCTGGCCAGCGACCACGAC TACAGGACACAGTATCACAAGTTCACAGCACTGCCCGAGGACCTGAAGATGGCCTGGGCCAAGAAAGCC CATGCCCTGCAGAGTGAGTTGCGCTACAAGTCAGACCTGATCGGCATGAAGGGCATAGGATGGCTGGCG CTGAGATCCCCACAGATGGAGAGTGCAAAGAAGGCTGGAGAACTCATCAGCGAGACCAAGTACCGTAAA AAACCAGACAGTATCAAGTTCACCACAGTGGTTGACTCCCCAGACCTGGTTCATGCCAAGAACAGCTAT ATGCACTGCAATGAGCGCATGTATAGATCTGGAGATGCAGAATCCCTGCACAGATACACCCTGATCCCC GACCATCCCGATTTCACCCGAGCTCGCCTCAATGCGCTGCATCTGAGTGACAAAGTCTACAGAAACTCC TGGGAGCAGACCCGGGCTGGCAGTTATGACTTCAGGCTGGATGCCATCCCCTTCCAGACTGCCCGGGCA TCTAGGGAGATCGCCAGTGATTTCCGGTACAAAGAGGCTTTCCTGCGGGACCGAGGCCTGCAGATCGGG TACCGCAGTGTCGACGATGACCCAAGGATGAAGCATTTCCTCAACGTTGGCAGGCTCCAGAGTGACAAT GAGTACAAGAAGGACTTTGCCAAGAGTCGGTCCCAGTTTCACAGCAGCACAGACCAGCCCGGCCTCCTT CAGGCCAAGAGGAGCCAGCAGCTGGCCAGTGATGTGCACTACAGGCAGCCCCTGCCCCAGCCCACCTGC GACCCGGAGCAGCTGGGCCTCAGGCATGCTCAGAAGGCCCACCAGCTGCAGAGTGATGTCAAGTATAAA TCAGACTTGAACCTGACCAGAGGTGTTGGCTGGACCCCTCCTGGCTCCTACAAAGTGGAAATGGCTCGG CGGGCTGCGGAACTGGCCAACGCAAGGGGCCTGGGTCTCCAGGGAGCTTACCAGCGGGGGGCAGAAGCA GTGGAGGCTGGAGATCATCAGAGTGGGGAGGTGAACCCAGATGCCACTGAGATTCTGCACGTCAAAAAG AAGAAGGCCCTGCTGTTGTGA
Restriction Sites	SgfI-RsrII
ACCN	NM_001261463
Insert Size	5196 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001261463.1
RefSeq Size	5549 bp
RefSeq ORF	5196 bp
Locus ID	4892
UniProt ID	Q86VF7
MW	197.2 kDa
Gene Summary	May be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) includes an alternate exon and uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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