MCM3 (NM_001270472) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HCC5; P1-MCM3; P1.h; RLFB
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332953 representing NM_001270472. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCTTATATGCTCCCAAGGTCCCCGCCTCTTCCTCGAGGTAACCTTTGGTGGCGGGAAGAGTTCGGAA GTTTTCGCGCCGGGGTGGAGTCATCCTGGGAACCTCCACGCGACTTTGGTGGAGGTAGTTCTTTGGCAG CGGGCATGGCGGGTACCGTGGTGCTGGACGATGTGGAGCTGCGGGAGGCTCAGAGAGATTACCTGGACT TCCTGGACGACGAGGCTTCTGAACAATGCCTTTGAGGAGCTGGTTGCCTTCCAGCGGGCCTTAAAGGAT TTTGTGGCCTCCATTGATGCTACCTATGCCAAGCAGTATGAGGAGTTCTACGTAGGACTGGAAGGCAGC TTTGGCTCCAAGCACGTCTCCCCGCGGACTCTTACCTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAG GGCATTGTCACTAAATGTTCTCTAGTTCGTCCCAAAGTCGTCCGCAGTGTCCACTACTGTCCTGCTACT AAGAAGACCATAGAGCGACGTTATTCTGATCTCACCACCCTGGTGGCCTTTCCCTCCAGCTCTGTCTAT CCTACCAAGGATGAGGAGAACAATCCCCTTGAGACAGAATATGGCCTTTCTGTCTACAAGGATCACCAG ACCATCACCATCCAGGAGATGCCGGAGAAGGCCCCAGCCGGCCAGCTCCCCCGCTCTGTGGACGTCATT CTGGATGATGACTTGGTGGATAAAGCGAAGCCTGGTGACCGGGTTCAGGTGGTGGGAACCTACCGTTGC CTTCCTGGAAAGAAGGGAGGCTACACCTCTGGGACCTTCAGGACTGTCCTGATTGCCTGTAATGTTAAG CAGATGAGCAAGGATGCTCAGCCCTCTTTCTCTGCTGAGGATATAGCCAAGATCAAGAAGTTCAGTAAA ACCCGATCCAAGGATATCTTTGACCAGCTGGCCAAGTCATTGGCCCCAAGTATCCATGGGCATGACTAT GTCAAGAAAGCAATCCTCTGCTTGCTCTTGGGAGGGGTGGAACGAGACCTAGAAAATGGCAGCCACATC CGTGGGGACATCAATATTCTTCTAATAGGAGACCCATCCGTTGCCAAGTCTCAGCTTCTGCGGTATGTG CTTTGCACTGCACCCCGAGCTATCCCCACCACTGGCCGGGGCTCCTCTGGAGTGGGTCTGACGGCTGCT GTCACCACAGACCAGGAAACAGGAGAGCGCCGTCTGGAAGCAGGGGCCATGGTCCTGGCTGACCGAGGC GTGGTTTGCATTGATGAATTTGACAAAATGTCTGACATGGATCGCACAGCCATCCATGAAGTGATGGAG CAGGGTCGAGTGACCATTGCCAAGGCTGGCATCCATGCTCGGCTGAATGCCCGCTGCAGTGTTTTGGCA GCTGCCAACCCTGTCTACGGCAGGTATGACCAGTATAAGACTCCAATGGAGAACATTGGGCTACAGGAC TCACTGCTGTCACGATTTGACTTGCTCTTCATCATGCTGGATCAGATGGATCCTGAGCAGGATCGGGAG ATCTCAGACCATGTCCTTCGGATGCACCGTTACAGAGCACCTGGGGAGCAGGATGGCGATGCTATGCCC TTGGGTAGTGCTGTGGATATCCTGGCCACAGATGATCCCAACTTTAGCCAGGAAGATCAGCAGGACACC CAGATTTATGAGAAGCATGACAACCTTCTACATGGGACCAAGAAGAAAAAGGAGAAGATGGTGAGTGCA GCATTCATGAAGAAGTACATCCATGTGGCCAAAATCATCAAGCCTGTCCTGACACAGGAGTCGGCCACC TACATTGCAGAAGAGTATTCACGCCTGCGCAGCCAGGATAGCATGAGCTCAGACACCGCCAGGACATCT CCAGTTACAGCCCGAACACTGGAAACTCTGATTCGACTGGCCACAGCCCATGCGAAGGCCCGCATGAGC AAGACTGTGGACCTGCAGGATGCAGAGGAAGCTGTGGAGTTGGTCCAGTATGCTTACTTTAAGAAGGTT CTGGAGAAGGAGAAGAAACGTAAGAAGCGAAGTGAGGATGAATCAGAGACAGAAGATGAAGAGGAGAAA AGCCAAGAGGACCAGGAGCAGAAGAGGAAGAGAAGGAAGACTCGCCAGCCAGATGCCAAAGATGGGGAT TCATACGACCCCTATGACTTCAGTGACACAGAGGAGGAAATGCCTCAAGTACACACTCCAAAGACGGCA GACTCACAGGAGACCAAGGAATCCCAGAAAGTGGAGTTGAGTGAATCCAGGTTGAAGGCATTCAAGGTG GCCCTCTTGGATGTGTTCCGGGAAGCTCATGCGCAGTCAATCGGCATGAATCGCCTCACAGAATCCATC AACCGGGACAGCGAAGAGCCCTTCTCTTCAGTTGAGATCCAGGCTGCTCTGAGCAAGATGCAGGATGAC AATCAGGTCATGGTGTCTGAGGGCATCATCTTCCTCATCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001270472
Insert Size	2457 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270472.1
RefSeq Size	3121 bp
RefSeq ORF	2457 bp
Locus ID	4172
UniProt ID	P25205
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways	Cell cycle, DNA replication
MW	91.9 kDa
Gene Summary	The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018] Transcript Variant: This variant (2) lacks an alternate exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.

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