RNF111 (NM_001270529) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARK; hRNF111
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332970 representing NM_001270529. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCTCAATGGACTCCTGAATATAACGAGCTCTACACCTTAAAAGTGGATATGAAGAGTGAGATTCCT TCTGATGCACCAAAGACACAGGAGAGTCTGAAAGGGATCCTTTTGCATCCAGAGCCCATTGGGGCAGCC AAAAGTTTTCCTGCAGGAGTTGAGATGATTAATAGTAAAGTGGGGAATGAATTCTCTCACCTGTGTGAT GATTCTCAAAAGCAAGAGAAGGAAATGAATGGTAACCAGCAAGAACAAGAAAAAAGTCTCGTTGTGAGG AAAAAACGCAAAAGCCAGCAGGCTGGCCCTTCGTATGTGCAGAATTGTGTTAAAGAAAACCAGGGAATA TTAGGACTGAGGCAACACCTAGGGACACCAAGTGATGAAGATAATGATTCCTCTTTTAGTGATTGTCTT TCTTCTCCTTCATCTAGTCTGCATTTTGGAGATTCTGATACTGTGACTTCAGATGAGGATAAAGAAGTC TCTGTAAGACATTCCCAGACCATTTTGAATGCTAAAAGTAGAAGCCATAGTGCACGGTCTCATAAGTGG CCTCGGACTGAGACAGAATCTGTATCGGGATTGTTAATGAAAAGACCCTGTTTACATGGCAGTTCGTTA CGGAGACTTCCATGCAGAAAGAGATTTGTAAAAAATAATTCCTCACAGAGGACACAGAAACAAAAAGAG AGGATATTAATGCAGAGGAAGAAACGAGAAGTGTTAGCTCGAAGAAAATATGCCTTGCTACCTAGTTCT AGTAGTTCCAGTGAGAATGACCTCAGCAGTGAATCCTCTTCTAGCTCATCAACTGAAGGAGAAGAAGAT TTGTTTGTTTCTGCCAGTGAAAACCACCAAAACAATCCAGCTGTTCCCTCAGGAAGTATTGATGAAGAT GTTGTGGTGATAGAAGCTTCCTCCACTCCCCAGGTTACTGCCAATGAAGAAATTAATGTTACCTCAACT GACAGTGAAGTGGAGATTGTAACAGTTGGAGAAAGCTATCGGTCTCGTTCAACCCTTGGACACTCCAGA TCTCATTGGAGCCAGGGTTCCAGTTCTCATGCAAGTCGGCCACAGGAGCCACGGAACCGCAGTAGGATT TCTACTGTTATACAGCCCTTGAGGCAGAATGCAGCAGAAGTTGTGGACCTTACCGTTGATGAAGATGAA CCTACTGTAGTACCAACCACTTCTGCAAGAATGGAATCACAAGCTACTAGCGCTTCCATTAACAATTCA AATCCATCTACCTCTGAGCAGGCCTCTGATACTGCTTCAGCTGTCACCAGTAGCCAACCTTCCACAGTG TCAGAGACTTCAGCTACTCTTACAAGCAATAGTACCACTGGCACTTCTATAGGAGATGACTCAAGGAGA ACTACATCTAGTGCTGTAACGGAAACTGGCCCTCCTGCAATGCCAAGGTTACCTTCCTGCTGTCCCCAG CACTCACCATGTGGAGGGTCGTCACAGAACCACCATGCATTAGGACATCCTCATACAAGTTGCTTTCAG CAGCATGGTCACCATTTTCAACATCATCACCACCACCACCATACTCCCCACCCAGCTGTCCCAGTTTCT CCTTCCTTTAGTGATCCTGCTTGCCCTGTGGAAAGACCTCCACAAGTACAAGCACCTTGTGGAGCAAAT AGTAGTTCTGGTACCAGCTATCATGAACAGGCATTGCCAGTGGACCTGAGCAACAGTGGTATCAGAAGT CATGGAAGTGGCAGTTTTCATGGAGCATCTGCATTTGACCCCTGCTGCCCTGTTTCTTCCTCCCGAGCT GCAATCTTTGGCCATCAGGCCGCTGCTGCTGCCCCAAGTCAACCTTTATCATCAATAGATGGCTATGGA TCAAGCATGGTTGCGCAGCCCCAGCCCCAGCCCCCTCCACAGCCCTCTCTCTCATCATGTCGACATTAC ATGCCACCCCCTTATGCCTCTTTGACAAGGCCACTTCATCATCAAGCTTCTGCCTGCCCGCATTCTCAT GGAAACCCCCCTCCTCAGACTCAGCCTCCGCCTCAAGTGGATTATGTTATTCCTCATCCTGTACATGCT TTCCATTCTCAAATATCTTCTCATGCAACATCTCATCCTGTGGCACCCCCACCACCAACTCACTTAGCC AGTACAGCTGCACCAATCCCTCAGCATCTTCCTCCTACACACCAGCCAATTTCGCACCATATTCCAGCC ACAGCACCTCCAGCACAGAGACTGCATCCTCATGAAGTGATGCAGAGGATGGAAGTTCAAAGGAGGAGG ATGATGCAGCATCCAACGCGGGCACATGAACGCCCCCCACCCCATCCACATAGGATGCACCCAAACTAT GGTCATGGGCATCATATTCATGTGCCTCAGACTATGTCCTCACATCCTCGACAGGCTCCAGAGAGGTCT GCCTGGGAACTGGGAATTGAAGCTGGAGTGACTGCAGCTACTTATACACCTGGTGCATTGCATCCTCAC TTGGCCCATTATCACGCACCTCCTCGACTTCATCACTTACAATTAGGAGCTCTTCCTTTAATGGTTCCT GATATGGCAGGCTATCCTCACATCCGTTACATTTCATCAGGATTGGATGGAACATCATTCAGAGGTCCT TTCAGGGGCAATTTTGAGGAACTGATTCATTTGGAAGAAAGATTAGGCAATGTCAATCGTGGAGCATCC CAGGGGACAATTGAAAGATGTACATATCCACATAAATACAAAAAGAGGAAACTGCACTGCAAACAAGAT GGGGAAGAAGGGACTGAGGAAGACACAGAGGAAAAATGTACTATCTGTTTGTCTATTTTAGAGGAAGGT GAAGATGTGAGACGTCTTCCATGTATGCACCTTTTCCACCAAGTGTGTGTTGACCAATGGTTGATTACC AATAAGAAGTGCCCCATATGCAGAGTGGACATTGAGGCCCAGCTGCCAAGTGAAAGTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001270529
Insert Size	2958 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270529.1
RefSeq Size	5899 bp
RefSeq ORF	2958 bp
Locus ID	54778
UniProt ID	Q6ZNA4
Protein Families	Druggable Genome
MW	107.8 kDa
Gene Summary	The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (3) lacks an in-frame segment in the 3' coding region, compared to variant 1, which results in a shorter protein (isoform 3), compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.