KIAA0090 (EMC1) (NM_001271428) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CAVIPMR; KIAA0090
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333054 representing NM_001271428. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGGCTGAGTGGGCTTCTCGTTTCTGGCTTTGGGCTACGCTGCTGATTCCTGCGGCCGCGGTCTAC GAAGACCAAGTGGGCAAGTTTGATTGGAGACAGCAATATGTTGGGAAGGTCAAGTTTGCCTCCTTGGAA TTTTCCCCTGGATCCAAGAAGTTGGTTGTAGCCACAGAGAAGAATGTGATTGCAGCATTAAATTCCCGA ACTGGGGAGATCTTGTGGCGCCATGTTGACAAGGGCACGGCAGAAGGGGCTGTGGATGCCATGCTGCTG CACGGACAGGATGTGATCACTGTGTCCAATGGAGGCCGAATCATGCGTTCCTGGGAGACTAACATCGGG GGCCTGAACTGGGAGATAACCCTGGACAGTGGCAGTTTCCAGGCACTTGGGCTGGTTGGCCTGCAGGAG TCTGTAAGGTACATCGCAGTCCTGAAGAAGACTACACTTGCCCTCCATCACCTCTCCAGTGGGCACCTC AAGTGGGTGGAACATCTCCCAGAAAGTGACAGCATCCACTACCAGATGGTGTATTCTTACGGCTCTGGG GTGGTGTGGGCCCTCGGAGTTGTTCCCTTCAGCCATGTGAACATTGTCAAGTTTAATGTGGAAGATGGA GAGATTGTTCAGCAGGTTAGGGTTTCAACTCCGTGGCTGCAGCACCTGTCTGGAGCCTGTGGTGTGGTG GATGAGGCTGTCCTGGTGTGTCCTGACCCGAGCTCACGTTCCCTCCAAACTTTGGCTCTGGAGACGGAA TGGGAGTTGAGACAGATCCCACTGCAGTCTCTCGACTTAGAATTTGGAAGTGGATTCCAACCCCGGGTC CTGCCTACCCAGCCCAACCCAGTGGACGCTTCCCGGGCCCAGTTCTTCCTGCACTTGTCCCCAAGCCAC TATGCTCTGCTGCAGTACCATTATGGAACGCTGAGTTTGCTTAAAAACTTCCCACAGACTGCCCTAGTG AGCTTTGCCACCACTGGGGAGAAGACGGTGGCTGCAGTCATGGCCTGTCGGAATGAAGTGCAGAAAAGT AGCAGTTCTGAAGATGGGTCAATGGGGAGCTTTTCGGAGAAGTCTAGTTCAAAGGACTCTCTGGCTTGC TTCAATCAGACCTACACCATTAACCTATACCTCGTGGAGACAGGTCGGCGGCTGCTGGACACCACGATA ACATTTAGCCTGGAACAGAGCGGCACTCGGCCTGAGCGGCTGTATATCCAGGTGTTCTTGAAGAAGGAT GACTCAGTGGGCTACCGGGCTTTGGTGCAGACAGAGGATCATCTGCTACTTTTCCTGCAGCAGTTGGGG AAGGTGGTGCTGTGGAGCCGTGAGGAGTCCCTGGCAGAAGTGGTGTGCCTAGAGATGGTGGACCTCCCC CTGACTGGGGCACAGGCCGAGCTGGAAGGAGAATTTGGCAAAAAGGCAGATGGCTTGCTGGGGATGTTC CTGAAACGCCTCTCGTCTCAGCTTATCCTGCTGCAAGCATGGACTTCCCACCTCTGGAAAATGTTTTAT GATGCTCGGAAGCCCCGGAGTCAGATTAAGAATGAGATCAACATTGACACCCTGGCCAGAGATGAATTC AACCTCCAGAAGATGATGGTGATGGTAACAGCCTCAGGCAAGCTTTTTGGCATTGAGAGCAGCTCTGGC ACCATCCTGTGGAAACAGTATCTACCCAATGTCAAGCCAGACTCCTCCTTTAAACTGATGGTCCAGAGA ACTACTGCTCATTTCCCCCATCCCCCACAGTGCACCCTGCTGGTGAAGGACAAGGAGTCGGGAATGAGT TCTCTGTATGTCTTCAATCCCATTTTTGGGAAGTGGAGTCAGGTAGCTCCCCCAGTGCTGAAGCGCCCC ATCTTGCAGTCCTTGCTTCTCCCAGTCATGGATCAAGACTACGCCAAGGTGTTGCTGTTGATAGATGAT GAATACAAGGTCACAGCTTTTCCAGCCACTCGGAATGTCTTGCGACAGCTACATGAGCTTGCCCCTTCC ATCTTCTTCTATTTGGTGGATGCAGAGCAGGGACGGCTGTGTGGATATCGGCTTCGAAAGGATCTCACC ACTGAGCTGAGTTGGGAGCTGACCATTCCCCCAGAAGTACAGCGGATCGTCAAGGTGAAGGGGAAACGC AGCAGTGAGCACGTTCATTCCCAGGGCCGTGTGATGGGGGACCGCAGTGTGCTCTACAAGAGCCTGAAC CCCAACCTGCTGGCCGTGGTGACAGAGAGCACAGACGCGCACCATGAGCGCACCTTTATTGGCATCTTC CTCATTGATGGCGTCACTGGGCGTATCATTCACTCCTCTGTGCAGAAGAAAGCCAAAGGCCCTGTCCAT ATCGTGCATTCAGAGAACTGGGTGGTGTACCAGTACTGGAACACCAAGGCTCGGCGCAACGAGTTTACC GTACTGGAGCTCTATGAGGGCACTGAGCAATACAACGCCACCGCCTTCAGCTCCCTGGACCGCCCCCAG CTGCCCCAGGTCCTCCAGCAGTCCTATATCTTCCCGTCCTCCATCAGTGCCATGGAGGCCACCATCACC GAACGGGGCATCACCAGCCGACACCTGCTGATTGGACTACCTTCTGGAGCAATTCTTTCCCTTCCTAAG GCTTTGCTGGATCCCCGCCGCCCCGAGATCCCAACAGAACAAAGCAGAGAGGAGAACTTAATCCCGTAT TCTCCAGATGTACAGATACACGCAGAGCGATTCATCAACTATAACCAGACAGTTTCTCGAATGCGAGGT ATCTACACAGCTCCCTCGGGTCTGGAGTCCACTTGTTTGGTTGTGGCCTATGGTTTGGACATTTACCAA ACTCGAGTCTACCCATCCAAGCAGTTTGACGTTCTGAAGGATGACTATGACTACGTGTTAATCAGCAGC GTCCTCTTTGGCCTGGTTTTTGCCACCATGATCACTAAGAGACTGGCACAGGTGAAGCTCCTGAATCGG GCCTGGCGATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271428
Insert Size	2979 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271428.1
RefSeq Size	6687 bp
RefSeq ORF	2979 bp
Locus ID	23065
UniProt ID	Q8N766
MW	111.7 kDa
Gene Summary	This gene encodes a single-pass type I transmembrane protein, which is a subunit of the endoplasmic reticulum membrane protein complex (EMC). Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2012] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central region, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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