MEGF8 (NM_001271938) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C19orf49; CRPT2; EGFL4; SBP1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333157 representing NM_001271938. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCCTGGGCAAGGTTCTGGCCATGGCACTGGTTTTGGCCTTGGCCGTGCTGGGGTCGCTGTCCCCT GGGGCCCGGGCGGGGGACTGCAAGGGGCAGCGGCAGGTGCTGCGGGAGGCGCCAGGCTTCGTGACGGAT GGTGCGGGCAACTACAGCGTCAATGGCAACTGCGAGTGGCTCATCGAGGCCCCAAGCCCCCAGCACCGG ATCCTGCTGGACTTCCTTTTCCTGGACACAGAGTGCACGTATGACTACCTGTTCGTGTATGACGGTGAC TCCCCGCGAGGGCCGCTGCTTGCCAGTCTAAGTGGGAGCACCCGACCTCCGCCCATCGAAGCTTCCTCA GGCAAGATGCTGCTGCACCTCTTCAGTGATGCCAACTACAACCTGCTGGGCTTTAACGCCTCATTCCGC TTCTCCCTGTGCCCGGGTGGCTGCCAGAGCCACGGGCAGTGCCAGCCACCGGGTGTGTGTGCCTGCGAG CCGGGCTGGGGGGGTCCTGACTGTGGCCTGCAGGAGTGCTCAGCCTACTGTGGCAGCCACGGCACCTGC GCCTCGCCCCTGGGACCATGCCGCTGTGAGCCTGGCTTCTTGGGACGTGCCTGTGACCTGCACCTGTGG GAGAACCAGGGGGCTGGGTGGTGGCACAACGTGAGTGCCAGGGACCCTGCCTTCTCTGCCCGTATTGGG GCAGCTGGCGCCTTCCTGTCCCCACCAGGGCTGCTGGCAGTTTTCGGAGGCCAGGACCTCAACAATGCC CTGGGTGACCTCGTCCTATACAACTTCTCCGCCAACACCTGGGAGTCTTGGGACCTGAGTCCTGCCCCG GCTGCCCGTCACTCCCATGTGGCCGTGGCCTGGGCCGGCTCCCTGGTACTGATGGGTGGTGAGCTGGCT GACGGCTCGCTCACCAACGACGTGTGGGCCTTCAGTCCACTGGGCAGGGGCCACTGGGAGCTCCTGGCA CCACCTGCCTCCAGCTCCTCGGGGCCCCCAGGCCTGGCAGGTCACGCGGCTGCCCTGGTGGATGATGTC TGGCTATATGTGTCTGGAGGCCGCACCCCGCACGACCTCTTCTCCTCTGGCCTCTTCCGTTTCCGCCTT GACAGCACCAGCGGGGGCTATTGGGAGCAGGTGATTCCGGCAGGCGGACGGCCCCCTGCTGCCACTGGC CACTCCATGGTGTTCCATGCCCCCTCCCGTGCCCTGCTGGTCCATGGTGGACACCGGCCCTCCACTGCC CGGTTCTCTGTGCGAGTGAACTCCACTGAGCTTTTCCACGTGGATCGGCATGTGTGGACGACGCTGAAG GGGCGGGATGGGCTTCAGGGCCCAAGGGAGCGAGCCTTCCACACAGCCAGTGTTCTGGGCAATTACATG GTGGTCTATGGGGGCAATGTGCACACCCATTACCAGGAGGAAAAGTGCTACGAAGATGGCATCTTCTTC TACCACCTTGGCTGCCATCAATGGGTGTCAGGAGCTGAGCTTGCCCCGCCAGGAACCCCTGAGGGCCGA GCAGCGCCTCCCAGTGGTCGGTACTCACATGTAGCTGCGGTGCTTGGTGGCAGCGTCCTGTTGGTGGCT GGGGGGTACAGCGGCCGGCCCCGTGGGGACTTGATGGCGTACAAGGTGCCCCCCTTTGTGTTCCAGGCA CCTGCCCCTGACTACCACTTGGACTACTGCTCCATGTACACAGACCACAGCGTCTGCTCCCGGGACCCG GAATGCAGTTGGTGCCAAGGAGCCTGCCAAGCTGCACCCCCTCCTGGGACCCCCTTGGGGGCTTGTCCA GCCGCCAGCTGCCTGGGCCTGGGCCGCCTCCTGGGTGACTGCCAGGCCTGCCTGGCCTTCAGCAGCCCC ACAGCCCCTCCACGGGGACCTGGCACCCTGGGCTGGTGCGTGCACAATGAGAGCTGCCTCCCTAGGCCT GAGCAGGCCCGCTGCCGAGGGGAGCAGATCTCAGGCACTGTGGGCTGGTGGGGGCCTGCGCCTGTCTTC GTCACGTCCCTGGAGGCCTGCGTCACCCAGAGCTTCCTGCCTGGCCTGCACTTGCTCACCTTTCAGCAG CCGCCCAATACCTCCCAGCCTGACAAGGTCTCAATTGTCCGCAGCACGACCATCACCCTAACACCCAGC GCAGAGACAGATGTGTCCCTGGTCTACCGTGGCTTCATCTACCCAATGCTGCCTGGAGGGCCAGGTGGA CCAGGGGCTGAGGACGTGGCCGTGTGGACGCGGGCCCAGCGCCTACACGTCCTGGCCCGGATGGCCCGT GGCCCTGACACGGAGAACATGGAGGAGGTGGGGCGCTGGGTGGCTCATCAGGAGAAGGAGACGCGGCGG CTGCAGCGCCCTGGGTCTGCTCGCCTCTTCCCTCTGCCTGGGCGGGACCACAAGTATGCAGTAGAGATC CAGGGCCAGCTCAATGGCTCGGCAGGCCCTGGGCACAGCGAGCTAACTCTGCTGTGGGATCGGACTGGT GTGCCAGGAGGCAGCGAGATCTCCTTCTTCTTCCTGGAGCCCTACCGCTCGTCGTCCTGCACCTCCTAT TCTTCCTGCCTGGGCTGCTTGGCAGACCAGGGCTGTGGCTGGTGCCTGACCAGTGCCACCTGCCACCTG CGCCAGGGCGGAGCCCATTGCGGGGATGACGGGGCTGGTGGGTCCCTGCTGGTGCTGGTGCCTACCCTC TGCCCACTCTGCGAGGAGCATCGGGACTGCCACGCCTGCACCCAGGACCCCTTCTGTGAGTGGCATCAG AGCACCAGCCGCAAAGGGGACGCGGCATGCAGCCGGCGGGGCCGGGGTCGGGGTGCCCTGAAGAGTCCA GAGGAGTGTCCCCCGCTCTGCAGCCAGCGACTGACCTGTGAGGACTGCCTGGCCAACTCTAGCCAGTGC GCCTGGTGCCAGTCCACCCACACCTGCTTCCTGTTTGCTGCCTACTTGGCCCGGTACCCACACGGGGGC TGTCGAGGCTGGGACGACAGTGTACACTCGGAGCCACGGTGCCGGAGCTGCGATGGCTTCCTGACCTGC CATGAGTGTCTGCAGAGCCACGAGTGTGGCTGGTGTGGCAATGAGGACAACCCCACACTGGGACGGTGC CTACAGGGGGACTTCTCAGGGCCCCTCGGTGGGGGTAACTGCTCCCTGTGGGTGGGGGAGGGCCTGGGG CTTCCCGTGGCCCTCCCTGCCCGCTGGGCATACGCCCGCTGTCCTGACGTGGATGAGTGTCGCCTGGGC CTGGCCCGGTGCCACCCGCGGGCGACCTGCCTGAACACGCCCCTCAGCTACGAGTGTCACTGCCAGCGG GGCTACCAGGGTGATGGCATCTCACACTGCAACCGCACGTGCTTGGAGGACTGTGGCCATGGTGTGTGC AGTGGCCCCCCGGACTTTACCTGCGTGTGTGACCTAGGCTGGACATCAGACCTGCCCCCTCCCACACCC GCCCCGGGTCCGCCAGCCCCCCGCTGCTCCCGGGACTGTGGCTGCAGCTTCCACAGCCACTGCCGCAAG CGGGGCCCTGGCTTCTGCGACGAGTGCCAGGACTGGACATGGGGGGAGCACTGCGAACGATGCCGGCCC GGCAGCTTCGGCAACGCCACAGGCTCTAGGGGCTGCCGGCCCTGCCAGTGCAACGGGCACGGGGACCCA CGCCGTGGCCACTGCGACAACCTCAGTGGGCTCTGCTTCTGCCAGGACCACACCGAGGGTGCCCACTGC CAGCTCTGCTCCCCAGGCTATTATGGGGATCCCCGGGCCGGTGGTTCCTGCTTTCGGGAGTGTGGGGGT CGCGCCCTCCTCACCAACGTGTCCTCAGTGGCACTGGGCTCACGCCGGGTCGGGGGGCTGCTGCCTCCA GGTGGCGGGGCTGCAAGAGCCGGGCCTGGCCTGTCCTACTGTGTGTGGGTTGTCTCGGCCACTGAGGAG CTACAGCCCTGTGCTCCCGGGACCCTCTGTCCCCCACTCACCCTCACCTTCTCCCCCGACAGCAGCACC CCCTGCACGCTGAGCTACGTCCTGGCGTTTGATGGATTCCCACGCTTCCTGGACACTGGTGTTGTCCAG TCGGACCGCAGCCTCATAGCTGCCTTCTGCGGCCAGCGACGGGACAGGCCCCTCACTGTTCAGGCCCTG TCTGGGCTGCTCGTGCTGCACTGGGAGGCCAATGGCTCCTCATCCTGGGGCTTCAATGCTTCGGTGGGC TCTGCCCGCTGTGGGTCAGGGGGCCCCGGGAGCTGTCCCGTCCCCCAGGAATGCGTGCCCCAGGACGGT GCTGCAGGTGCGGGGCTCTGCCGATGTCCTCAGGGCTGGGCTGGCCCACACTGCCGCATGGCTCTGTGT CCTGAGAACTGCAATGCCCACACTGGGGCAGGAACTTGTAACCAGAGCCTGGGTGTGTGCATCTGTGCC GAGGGCTTCGGGGGCCCCGACTGCGCCACCAAGCTGGATGGCGGGCAGCTGGTCTGGGAGACCCTCATG GACAGCCGCCTCTCAGCCGACACTGCCAGCCGCTTCCTGCACCGCCTGGGCCACACCATGGTGGATGGA CCCGATGCCACCTTGTGGATGTTTGGGGGCCTGGGCCTGCCCCAGGGGCTGCTGGGAAACCTGTACAGG TACTCAGTGAGTGAGCGGCGGTGGACACAGATGCTGGCGGGAGCCGAGGACGGGGGCCCAGGCCCATCG CCCCGCTCCTTCCATGCAGCCGCATATGTGCCCGCTGGCCGTGGTGCCATGTATCTGCTGGGGGGACTT ACCGCTGGAGGCGTCACCCGTGATTTCTGGGTCCTCAACCTCACCACCCTGCAATGGCGGCAGGAGAAG GCCCCCCAGACCGTGGAGCTGCCAGCCGTTGCTGGTCACACCCTTACTGCCCGCCGAGGCCTGTCTCTG CTCCTGGTGGGCGGTTACTCCCCGGAAAATGGCTTCAACCAGCAGCTGCTGGAGTACCAGCTGGCAACC GGCACCTGGGTGTCAGGAGCCCAGAGTGGGACACCCCCCACAGGTCTCTATGGTCACTCTGCTGTCTAC CACGAGGCCACCGACTCCCTCTACGTGTTTGGGGGGTTCCGATTCCATGTGGAGCTGGCGGCCCCATCC CCCGAGCTCTACTCCCTGCACTGTCCTGACCGCACCTGGAGTCTGCTGGCCCCTTCTCAGGGGGCAAAG CGAGATCGTATGAGGAATGTGCGTGGCTCATCTCGGGGTCTGGGCCAAGTTCCTGGGGAGCAGCCTGGG TCATGGGGGTTCCGGGAAGTCAGGAAGAAGATGGCTCTGTGGGCTGCTCTTGCTGGTACAGGAGGTTTC CTGGAGGAAATCTCACCTCACCTGAAGGAGCCCCGCCCCCGGCTTTTCCACGCCTCAGCCCTGTTAGGG GACACCATGGTGGTTCTTGGGGGGCGCTCGGACCCTGACGAGTTCAGCAGCGACGTTCTGCTCTACCAG GTCAACTGCAATGCCTGGCTTCTGCCCGACCTCACCCGCTCGGCCTCTGTGGGGCCCCCAATGGAGGAG TCTGTGGCCCATGCTGTGGCAGCAGTCGGGAGCCGCCTGTATATCTCTGGGGGTTTCGGGGGAGTGGCC CTGGGCCGCCTGCTGGCACTGACCCTGCCCCCTGACCCCTGCCGCCTGCTGTCCTCACCTGAAGCTTGT AACCAGTCTGGGGCCTGCACCTGGTGCCATGGGGCCTGCTTGTCCGGGGATCAGGCCCACAGGCTGGGC TGCGGGGGCTCCCCCTGCTCCCCAATGCCTCGCTCCCCGGAGGAATGTCGACGTCTCCGGACCTGCAGT GAGTGCCTGGCCCGCCATCCTCGGACCCTGCAACCTGGAGATGGAGAGGCGTCCACCCCCCGCTGTAAG TGGTGTACCAACTGCCCCGAAGGTGCTTGCATTGGACGCAATGGGTCCTGCACCTCTGAGAATGACTGT CGGATCAACCAGCGAGAGGTCTTCTGGGCAGGGAACTGCTCCGAGGCTGCGTGCGGGGCTGCTGACTGC GAGCAGTGCACGCGGGAGGGCAAGTGCATGTGGACGCGGCAGTTCAAGAGGACAGGGGAGACCCGCCGC ATCCTCTCCGTGCAGCCCACCTATGACTGGACGTGCTTCAGCCACTCTCTGCTGAATGTGTCCCCCATG CCGGTGGAATCATCACCCCCACTGCCCTGCCCCACCCCTTGTCACCTCCTACCCAACTGTACCTCCTGC CTGGACTCTAAGGGAGCAGATGGGGGCTGGCAGCACTGTGTTTGGAGCAGCAGCCTGCAGCAGTGTCTG AGCCCTTCCTACCTGCCCCTGCGATGTATGGCCGGAGGCTGTGGGCGGCTGCTCCGGGGACCTGAGAGC TGCTCCCTGGGCTGTGCTCAGGCAACTCAGTGCGCCTTGTGCCTGCGGCGCCCCCATTGCGGCTGGTGT GCCTGGGGGGGCCAGGATGGGGGTGGCCGCTGCATGGAGGGTGGACTCAGCGGCCCCCGTGATGGGCTG ACATGTGGGCGTCCGGGGGCCTCCTGGGCCTTCCTGTCCTGCCCCCCTGAGGACGAGTGTGCAAACGGG CACCACGACTGCAACGAGACGCAGAATTGCCACGACCAGCCCCACGGCTATGAGTGCAGCTGCAAGACC GGCTATACCATGGACAACATGACAGGGCTGTGCCGCCCTGTGTGCGCCCAGGGCTGCGTGAACGGCTCA TGTGTGGAGCCCGACCACTGCCGCTGCCACTTTGGCTTTGTGGGCCGCAACTGCTCCACGGAATGCCGC TGCAACCGCCACAGTGAATGCGCTGGTGTTGGGGCGCGTGACCACTGCTTGCTCTGCCGCAACCACACC AAGGGCAGCCACTGTGAGCAGTGCCTCCCGCTGTTTGTGGGTTCAGCTGTCGGAGGCGGGACCTGCCGG CCCTGCCACGCCTTTTGTCGTGGAAATAGCCACATCTGCATCTCCAGGAAGGAGTTACAAATGTCCAAG GGAGAGCCAAAGAAGTACTCACTGGACCCAGAGGAGATTGAAAACTGGGTGACAGAGGGTCCTAGTGAA GACGAGGCCGTGTGCGTGAACTGCCAGAATAACAGCTATGGGGAGAAATGCGAGAGCTGCCTGCAGGGC TACTTCCTCCTGGACGGGAAGTGCACCAAATGCCAGTGTAATGGCCACGCGGACACATGTAACGAGCAG GATGGGACGGGCTGTCCATGTCAGAATAACACAGAGACGGGCACATGCCAGGGCAGCTCCCCCAGTGAC CGTCGAGACTGCTACAAGTACCAGTGCGCCAAGTGCCGGGAATCATTTCACGGGAGTCCGCTGGGCGGC CAGCAGTGCTACCGCCTCATCTCGGTGGAGCAGGAGTGCTGCCTGGACCCCACGTCCCAGACCAACTGC TTCCATGAGCCCAAACGCCGGGCGCTAGGCCCCGGCCGCACTGTCCTCTTTGGCGTGCAGCCCAAATTC ACCAACGTGGACATCCGCCTGACGCTGGACGTGACCTTCGGGGCCGTGGACCTCTATGTCTCCACCTCC TATGACACCTTCGTGGTCCGTGTGGCCCCTGACACTGGCGTCCATACTGTACACATCCAGCCACCCCCA GCCCCACCACCTCCACCACCCCCTGCAGATGGTGGGCCCCGGGGGGCTGGGGATCCAGGAGGAGCAGGG GCCAGCAGTGGGCCGGGCGCCCCAGCAGAGCCACGGGTACGGGAGGTATGGCCGCGGGGCCTGATTACC TACGTGACGGTGACGGAGCCGTCGGCAGTGCTGGTGGTCCGCGGCGTGCGGGACCGGCTGGTCATCACC TACCCACACGAGCACCATGCCCTCAAGTCGAGCCGCTTCTACCTGCTGCTGCTGGGCGTGGGAGACCCA AGTGGGCCCGGCGCCAACGGCTCAGCCGACTCGCAGGGCCTGCTCTTCTTCCGGCAGGACCAGGCCCAC ATTGACCTGTTTGTCTTCTTCTCCGTCTTCTTCTCCTGCTTCTTCCTCTTCCTCTCACTCTGTGTGCTC CTCTGGAAGGCCAAGCAGGCTCTGGACCAGCGGCAGGAGCAGCGCCGGCACTTGCAGGAGATGACCAAG ATGGCCAGCCGCCCCTTCGCCAAGGTCACCGTCTGCTTCCCACCTGACCCTACTGCCCCGGCCTCCGCC TGGAAGCCGGCTGGGCTCCCACCTCCCGCCTTCCGCCGCTCTGAGCCCTTCCTGGCACCCCTGCTGCTG ACAGGGGCCGGTGGGCCCTGGGGACCCATGGGAGGGGGCTGCTGCCCACCAGCCATCCCCGCCACCACT GCTGGGCTGCGAGCTGGGCCCATCACTCTCGAGCCCACAGAAGATGGCATGGCTGGCGTGGCCACACTG CTGCTCCAGCTGCCTGGCGGGCCCCATGCACCCAACGGCGCCTGCCTGGGGTCAGCCCTCGTCACACTG CGGCACAGGCTGCACGAGTACTGTGGGGGTGGTGGGGGTGCTGGGGGCAGTGGGCATGGGACTGGTGCG GGCCGGAAGGGACTGTTGAGCCAGGACAACCTCACCAGCATGTCCCTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001271938
Insert Size	8538 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271938.1
RefSeq Size	11167 bp
RefSeq ORF	8538 bp
Locus ID	1954
UniProt ID	Q7Z7M0
Protein Families	Druggable Genome, Transmembrane
MW	303.1 kDa
Gene Summary	The protein encoded by this gene is a single-pass type I membrane protein of unknown function that contains several EGF-like domains, Kelch repeats, and PSI domains. Defects in this gene are a cause of Carpenter syndrome 2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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