TSPEAR (NM_001272037) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C21orf29; DFNB98; ECTD14; TSP-EAR
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333190 representing NM_001272037. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGCTTCCCAGCATCCAGGATTTTCTCCCAGTGTGACCTCTTCCCTGAAGAATTTTCCATCGTCGTA ACTTTGAGAGTTCCCAATCTTCCACCCAAGAGGAACGAGTACCTGCTGACGGTGGTGGCAGAGGAGAGC GACCTGCTGCTGCTCGGCCTGCGGTTGTCACCTGCCCAGCTGCACTTCCTGTTCCTTCGCGAGGACACG GCCGGCGCCTGGCAGACCCGAGTGTCCTTCCGCAGCCCGGCCCTGGTGGATGGCCGCTGGCACACACTG GTCCTGGCTGTGTCCGCAGGCGTCTTCTCCCTCACCACGGACTGCGGCCTCCCGGTGGACATAATGGCC GATGTGCCCTTCCCAGCCACCCTGTCAGTGAAAGGAGCTCGATTCTTCGTCGGCAGCCGGAGGAGAGCC AAAGGCCTGTTCATGGGACTGGTGAGGCAACTGGTCCTGCTGCCGGGCTCAGACGCCACCCCAAGGCTG TGTCCCAGCAGGAACGCCCCGCTGGCGGTGCTGTCCATCCCACGGGTCCTGCAGGCTCTCACGGGGAAG CCAGAAGATAACGAGGTGCTAAAATATCCCTATGAAACCAACATTCGAGTGACGCTGGGACCCCAGCCA CCGTGTACCGAGGTGGAAGACGCCCAGTTCTGGTTTGATGCCAGCCGGAAGGGCCTGTATCTGTGTGTT GGCAACGAGTGGGTCTCCGTGTTAGCAGCCAAAGAAAGACTGGACTACGTGGAGGAGCATCAGAACTTG TCCACCAACTCAGAGACCCTGGGCATTGAGGTGTTCCGCATCCCTCAGGTGGGGCTCTTTGTGGCCACA GCCAATCGCAAAGCCACATCCGCCGTCTACAAGTGGACCGAAGAGAAGTTCGTCTCATATCAGAACATC CCCACGCACCAAGCACAGGCCTGGAGGCATTTCACCATCGGGAAAAAGATCTTCCTGGCAGTGGCTAAT TTTGAACCAGATGAGAAGGGTCAGGAGTTCTCTGTCATTTACAAATGGAGCCACAGAAAGCTGAAGTTT ACCCCATATCAGAGCATTGCCACACACAGCGCCCGAGACTGGGAGGCCTTCGAGGTGGATGGGGAGCAC TTCCTGGCGGTGGCCAACCACCGGGAAGGCGACAACCACAACATCGACAGTGTCATCTACAAGTGGAAC CCGGCAACCCGGCTCTTCGAGGCCAACCAGACCATCGCCACCTCCGGCGCCTACGACTGGGAGTTCTTC AGTGTGGGGCCCTACTCGTTCCTGGTGGTGGCCAACACCTTCAACGGCACCTCCACCAAGGTGCACTCG CACCTCTACATCCGACTCCTGGGCTCCTTCCAGCTCTTCCAGTCCTTCCCGACGTTCGGTGCTGCAGAC TGGGAGGTCTTCCAGATCGGGGAGAGGATCTTCCTCGCTGTGGCAAACAGTCACAGCTACGATGTGGAG ATGCAAGTCCAGAATGATTCCTATGTCATCAACTCCGTCATCTACGAGCTGAACGTGACCGCGCAGGCC TTTGTCAAGTTCCAGGACATTCTCACCTGCAGTGCTCTGGACTGGGAGTTTTTCTCGGTGGGAGAAGAT TATTTCCTGGTGGTGGCCAACTCCTTCGATGGGCGTACCTTCTCGGTGAACAGTATTATTTACAGGTGG CAGGGCTACGAGGGCTTCGTGGCGGTGCACAGCCTCCCCACCGTCGGCTGCAGGGACTGGGAGGCCTTC AGCACCACGGCTGGTGCCTACCTCATCTACTCCAGCGCCAAGGAGCCCCTCTCCAGGGTCCTGCGGCTG AGGACACGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001272037
Insert Size	1806 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001272037.1
RefSeq Size	4042 bp
RefSeq ORF	1806 bp
Locus ID	54084
UniProt ID	Q8WU66
MW	67.9 kDa
Gene Summary	This gene encodes a protein that contains a N-terminal thrombospondin-type laminin G domain and several tandem arranged epilepsy-associated repeats (EARs). A mutation in this gene is the cause of autosomal recessive deafness-98. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (2) contains an additional exon in the 5' region that causes translation initiation from a downstream AUG compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1.

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