Hsp75 (TRAP1) (NM_001272049) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HSP 75; HSP75; HSP90L; TRAP-1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333193 representing NM_001272049. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGCGCGAGCTGCGGGCGCTGCTGCTGTGGGGCCGCCGCCTGCGGCCTTTGCTGCGGGCGCCGGCG CTGGCGGCCGTGCCGGGAGGTTCCACTTCCAAACATGAGTTCCAGGCCGAGACAAAGAAGCTTTTGGAC ATTGTTGCCCGGTCCCTGTACTCAGAAAAAGAGGTGTTTATACGGGAGCTGATCTCCAATGCCAGCGAT GCCTTGGAAAAACTGCGTCACAAACTGGTGTCTGACGGCCAAGCACTGCCAGAAATGGAGATTCACTTG CAGACCAATGCCGAGAAAGGCACCATCACCATCCAGGATACTGGTATCGGGATGACACAGGAAGAGCTG GTGTCCAACCTGGGGACGATTGCCAGATCGGGGTCAAAGGCCTTCCTGGATGCTCTGCAGAACCAGGCT GAGGCCAGCAGCAAGATCATCGGCCAGTTTGGAGTGGGTTTCTACTCAGCTTTCATGGTGGCTGACAGA GTGGAGGTCTATTCCCGCTCGGCAGCCCCGGGGAGCCTGGGTTACCAGTGGCTTTCAGATGGTTCTGGA GTGTTTGAAATCGCCGAAGCTTCGGGAGTTAGAACCGGGACAAAAATCATCATCCACCTGAAATCCGAC TGCAAGGAGTTTTCCAGCGAGGCCCGGGTGCGAGATGTGGTAACGAAGTACAGCAACTTCGTCAGCTTC CCCTTGTACTTGAATGGAAGGCGGATGAACACCTTGCAGGCCATCTGGATGATGGACCCCAAGGATGTC CGTGAGTGGCAACATGAGGAGTTCTACCGCTACGTCGCGCAGGCTCACGACAAGCCCCGCTACACCCTG CACTATAAGACGGACGCACCGCTCAACATCCGCAGCATCTTCTACGTGCCCGACATGAAACCGTCCATG TTTGATGTGAGCCGGGAGCTGGGCTCCAGCGTTGCACTGTACAGCCGCAAAGTCCTCATCCAGACCAAG GCCACGGACATCCTGCCCAAGTGGCTGCGCTTCATCCGAGGTGTGGTGGACAGTGAGGACATTCCCCTG AACCTCAGCCGGGAGCTGCTGCAGGAGAGCGCACTCATCAGGAAACTCCGGGACGTTTTACAGCAGAGG CTGATCAAATTCTTCATTGACCAGAGTAAAAAAGATGCTGAGAAGTATGCAAAGTTTTTTGAAGATTAC GGCCTGTTCATGCGGGAGGGCATTGTGACCGCCACCGAGCAGGAGGTCAAGGAGGACATAGCAAAGCTG CTGCGCTACGAGTCCTCGGCGCTGCCCTCCGGGCAGCTAACCAGCCTCTCAGAATACGCCAGCCGCATG CGGGCCGGCACCCGCAACATCTACTACCTGTGCGCCCCCAACCGTCACCTGGCAGAGCACTCACCCTAC TATGAGGCCATGAAGAAGAAAGACACAGAGGTTCTCTTCTGCTTTGAGCAGTTTGATGAGCTCACCCTG CTGCACCTTCGTGAGTTTGACAAGAAGAAGCTGATCTCTGTGGAGACGGACATAGTCGTGGATCACTAC AAGGAGGAGAAGTTTGAGGACAGGTCCCCAGCCGCCGAGTGCCTATCAGAGAAGGAGACGGAGGAGCTC ATGGCCTGGATGAGAAATGTGCTGGGGTCGCGTGTCACCAACGTGAAGGTGACCCTCCGACTGGACACC CACCCTGCCATGGTCACCGTGCTGGAGATGGGGGCTGCCCGCCACTTCCTGCGCATGCAGCAGCTGGCC AAGACCCAGGAGGAGCGCGCACAGCTCCTGCAGCCCACGCTGGAGATCAACCCCAGGCACGCGCTCATC AAGAAGCTGAATCAGCTGCGCGCAAGCGAGCCTGGCCTGGCTCAGCTGCTGGTGGATCAGATATACGAG AACGCCATGATTGCTGCTGGACTTGTTGACGACCCTAGGGCCATGGTGGGCCGCTTGAATGAGCTGCTT GTCAAGGCCCTGGAGCGACACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001272049
Insert Size	1956 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001272049.1
RefSeq Size	2153 bp
RefSeq ORF	1956 bp
Locus ID	10131
UniProt ID	Q12931
Protein Families	Druggable Genome
MW	74.3 kDa
Gene Summary	This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter than isoform 1.

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