PKD1L2 (NM_001278423) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PC1L2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335235 representing NM_001278423. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGGAGGACTCTCCAGTGGCTATGTTCAGCTGGTATTTGGACAACACCCCAACAGAGCAGGCTGAG CCCCTCCTGGATGCCTGCAGACTCAGAGGATTTTGGCCAAGGTCCTTAACCCTCCTCCAGAGCAACACC TCCACGTTGCTGTTGAACAGCTCGTTTCTGCAGTCCCGGGGAGAGGTCATCCGAATCAGAGCCACAGCA CTGACCAGGCATGCCTATGGGGAGGACACCTATGTGATCAGCACTGTGCCTCCCCGTGAGGTGCCTGCC TGCACTATTGCCCCAGAGGAGGGCACCGTTCTGACGAGCTTTGCCATCTTCTGCAACGCCTCCACAGCC CTGGGACCCCTGGAGTTCTGCTTCTGTCTGGAATCAGGTTCCTGCCTACACTGTGGCCCTGAACCTGCC CTCCCATCAGTGTATCTGCCACTTGGAGAGGAGAACAATGACTTTGTGCTGACAGTAGTTATTTCTGCC ACCAATCGTGCAGGGGACACGCAGCAGACCCAGGCCATGGCTAAGGTGGCACTCGGAGACACATGTGTT GAGGATGTAGCATTCCAGGCTGCCGTGTCAGAGAAAATCCCCACAGCTCTGCAAGGCGAGGGTGGCCCC GAGCAGCTCCTCCAGCTGGCCAAGGCTGTGTCCTCCATGCTGAACCAAGAGCATGAAAGCCAGGGCTCA GGACAGTCACTGAGCATAGACGTCAGACAGAAGGTCAGAGAGCATGTGCTGGGATCACTGTCTGCAGTC ACCACCGGCTTGGAGGACGTGCAGAGGGTGCAGGAGCTGGCCGAGGTGCTGAGAGAGGTGACCTGCCGG AGTAAGGAACTCACACCCTCGGCCCAGGGGTCCTGCATGGGCGATTCATGGGAAGGTGCCCCTCCTGCT GCCCATGTATCTCACGCTAGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278423
Insert Size	921 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278423.1
RefSeq Size	1533 bp
RefSeq ORF	921 bp
Locus ID	114780
UniProt ID	Q7Z442
Protein Families	Druggable Genome
MW	32.8 kDa
Gene Summary	This gene encodes a member of the polycystin protein family. The encoded protein contains 11 transmembrane domains, a latrophilin/CL-1-like GPCR proteolytic site (GPS) domain, and a polycystin-1, lipoxygenase, alpha-toxin (PLAT) domain. This protein may function as a component of cation channel pores. This gene appears to be a polymorphic pseudogene in humans, and the reference genome encodes a non-functional allele. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2019] Transcript Variant: This variant (5) lacks several 5' and 3' exons but includes alternate 5' and 3' exons, and it thus differs in both UTRs, uses a downstream in-frame start codon and has an alternate 3' coding region, compared to variant 1. The encoded isoform (e) is shorter and has a distinct C-terminus, compared to isoform a.

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