PKNOX1 (NM_001286258) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	pkonx1c; PREP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335310 representing NM_001286258. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTAAAAGCAATCCAGGTTTTGCGCATTCATCTTCTTGAGCTGGAAAAGGTTAACGAACTCTGCAAA GATTTCTGCAGTCGATACATTGCTTGTCTGAAAACAAAAATGAACAGTGAAACTCTGTTGAGTGGAGAG CCTGGAAGCCCGTACTCACCAGTGCAGTCCCAGCAGATTCAAAGTGCCATCACAGGCACCATCAGCCCT CAGGGAATTGTGGTGCCGGCGTCCGCGCTGCAGCAGGGAAACGTAGCCATGGCGACGGTGGCAGGTGGC ACAGTGTATCAGCCTGTCACGGTCGTCACTCCCCAAGGCCAAGTGGTCACACAGACATTGTCGCCTGGG ACAATTAGGATCCAGAACTCCCAGCTTCAGTTACAGTTAAACCAAGATCTCAGCATCTTGCATCAAGAT GATGGTTCATCTAAGAACAAGAGGGGCGTCCTGCCAAAGCATGCCACGAACGTGATGCGGTCCTGGCTC TTCCAGCACATCGGGCATCCCTACCCAACAGAGGATGAGAAAAAACAGATTGCTGCTCAGACAAATTTG ACACTACTCCAAGTCAACAACTGGTTCATCAATGCCAGAAGACGAATTCTTCAGCCAATGTTGGATTCA AGTTGTTCAGAGACCCCCAAAACAAAGAAAAAAACTGCTCAGAACCGGCCAGTTCAGAGGTTTTGGCCT GATTCTATTGCATCAGGAGTCGCACAGCCACCGCCGAGCGAGCTCACCATGTCGGAAGGAGCTGTTGTC ACCATCACCACGCCCGTGAACATGAACGTGGACAGCCTTCAGTCTCTGTCCTCGGACGGGGCCACCCTG GCGGTGCAGCAGGTCATGATGGCAGGGCAGAGCGAGGACGAGTCTGTGGACAGCACAGAGGAGGATGCG GGTGCCCTGGCCCCTGCCCACATCAGCGGGCTGGTCTTGGAGAACAGTGACTCCCTGCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286258
Insert Size	960 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286258.1
RefSeq Size	5180 bp
RefSeq ORF	960 bp
Locus ID	5316
Protein Families	Transcription Factors
MW	34.5 kDa
Gene Summary	Activates transcription in the presence of PBX1A and HOXA1.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an exon in the 5' region and initiates translation from an in-frame downstream start codon compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1.

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