MAPK4 (NM_001292039) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ERK-4; ERK4; p63-MAPK; p63MAPK; PRKM4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335728 representing NM_001292039. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGGGCCGCCGGCTGCATCCTGGCTGAGATGCTTACGGGGAGAATGCTCTTTGCTGGGGCCCATGAG CTGGAGCAGATGCAACTCATCCTGGAGACCATCCCTGTAATCCGGGAGGAAGACAAGGACGAGCTGCTC AGGGTGATGCCTTCCTTTGTCAGCAGCACCTGGGAGGTGAAGAGGCCTCTGCGCAAGCTGCTCCCTGAA GTGAACAGTGAAGCCATCGACTTTCTGGAGAAGATCCTGACCTTTAACCCCATGGATCGCCTAACAGCT GAGATGGGGCTGCAACACCCCTACATGAGCCCATACTCGTGCCCTGAGGACGAGCCCACCTCACAACAC CCCTTCCGCATTGAGGATGAGATCGACGACATCGTGCTGATGGCCGCTAACCAGAGCCAGCTGTCCAAC TGGGACACGTGCAGTTCCAGGTACCCTGTGAGCCTGTCGTCGGACCTGGAGTGGCGGCCTGACCGGTGC CAGGACGCCAGCGAGGTACAGCGCGACCCGCGCGCGGGTTCGGCGCCACTGGCTGAGGACGTGCAGGTG GACCCGCGCAAGGACTCGCACAGCAGCTCCGAGCGCTTCCTAGAGCAGTCGCACTCGTCCATGGAGCGC GCCTTCGAGGCCGACTACGGGCGCTCCTGCGACTACAAGGTGGGGTCGCCGTCCTACCTGGACAAGCTG CTGTGGCGCGACAACAAGCCGCACCACTACTCGGAGCCCAAGCTCATCCTGGACCTGTCGCACTGGAAG CAGGCGGCCGGCGCGCCCCCCACGGCCACGGGGCTGGCGGACACGGGGGCGCGCGAGGACGAGCCGGCC AGCCTCTTCCTGGAGATCGCGCAGTGGGTCAAGAGCACGCAGGGCGGCCCAGAGCACGCCAGCCCGCCC GCCGACGACCCCGAGCGCCGCTTGTCTGCCTCGCCCCCCGGCCGCCCGGCCCCGGTGGACGGCGGCGCC AGCCCCCAGTTCGACCTGGACGTGTTCATCTCCCGCGCCCTGAAGCTCTGCACCAAGCCCGAGGACCTG CCGGACAATAAACTGGGCGACCTCAATGGTGCGTGCATCCCCGAGCACCCTGGCGACCTCGTGCAGACC GAGGCCTTCTCCAAAGAAAGGTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001292039
Insert Size	1131 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001292039.1
RefSeq Size	3335 bp
RefSeq ORF	1131 bp
Locus ID	5596
UniProt ID	P31152
Protein Families	Druggable Genome, Protein Kinase
MW	42.1 kDa
Gene Summary	Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus and phosphorylate nuclear targets. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] Transcript Variant: This variant (2) lacks part of the 5' coding region and uses a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.

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