NHEDC2 (SLC9B2) (NM_001300756) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NHA2; NHE10; NHEDC2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335999 representing NM_001300756. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGGATGAAGATAAAAGAATTACATATGAAGATTCAGAACCATCCACAGGAATGAATTACACGCCC TCCATGCATCAAGAAGCACAGGAGGAGACAGTTATGAAGCTCAAAGGTATAGATGCAAATGAACCAACA GAAGGAAGTATTCTTTTGAAAAGCAGTGAAAAAAAGCTACAAGAAACACCAACTGAAGCAAATCACGTA CAAAGACTGAGACAAATGCTGGCTTGCCCTCCACATGGTTTACTGGACAGGGTCATAACAAATGGCATG CTGCTTGCAGGGTTTCTCATCAGAAATATCCCAGTCATCAACGATAATGTGCAGATCAAGCACAAGTGG TCTTCCTCTTTGAGAAGCATAGCCCTGTCTATCATTCTGGTTCGTGCTGGCCTTGGTCTGGATTCAAAG GCCCTGAAGAAGTTAAAGGGCGTTTGTGTAAGACTGTCCATGGGTCCCTGTATTGTGGAGGCGTGCACA TCTGCTCTTCTTGCCCATTACCTGCTGGGTTTACCATGGCAATGGGGATTTATACTGGGTTTTGTTTTA GGTGCTGTATCTCCAGCTGTTGTGGTGCCTTCAATGCTCCTTTTGCAGGGAGGAGGCTATGGTGTTGAG AAGGGTGTCCCAACCTTGCTCATGGCAGCTGGCAGCTTCGATGACATTCTGGCCATCACTGGCTTCAAC ACATGCTTGGGCATAGCCTTTTCCACAGGCTCTACTGTCTTTAATGTCCTCAGAGGAGTTTTGGAGGTG GTAATTGGTGTGGCAACTGGATCTGTTCTTGGATTTTTCATTCAGTACTTTCCAAGCCGTGACCAGGAC AAACTTGTGTGTAAGAGAACATTCCTTGTGTTGGGGTTGTCTGTGCTAGCTGTGTTCAGCAGTGTGCAT TTTGGTTTCCCTGGATCAGGAGGACTGTGCACGTTGGTCATGGCTTTCCTTGCAGGCATGGGATGGACC AGCGAAAAGGCAGAGGTTGAAAAGATAATTGCAGTTGCCTGGGACATTTTTCAGCCCCTTCTTTTTGGA CTAATTGGAGCAGAGGTATCTATTGCATCTCTCAGACCAGAAACTGTAGGCTGCAATAGGATCTGTGGC TTTGGACACAGCAAGGTCACATGGAGAGAAACAATTAGAAGACTATGGAATGGATGTGTTGACAGTGGC ATTTTTGTCCATCCTCATCACAGCCCCAATTGGAAGTCTGCTTATTGGTTTACTGGGCCCCAGGCTTCT GCAGAAAGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300756
Insert Size	1254 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300756.1
RefSeq Size	2833 bp
RefSeq ORF	1254 bp
Locus ID	133308
UniProt ID	Q86UD5
Protein Families	Druggable Genome, Transmembrane
MW	45.1 kDa
Gene Summary	Sodium hydrogen antiporters, such as NHEDC2, convert the proton motive force established by the respiratory chain or the F1F0 mitochondrial ATPase into sodium gradients that drive other energy-requiring processes, transduce environmental signals into cell responses, or function in drug efflux (Xiang et al., 2007 [PubMed 18000046]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (3) lacks two alternate exons in the central and 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1.

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