LILRA1 (NM_001278319) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CD85I; LIR-6; LIR6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336136 representing NM_001278319. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCCCCATCGTCACAGTCCTGATCTGTCTCAGGCTGAGTCTGGGCCCCCGGACCCACGTGCAGGCA GGGACCCTCCCCAAGCCCACACTCTGGGCTGAGCCAGGCTCTGTGATCACCCAGGGGAGTCCCGTGACC CTCTGGTGTCAGGGGATCCTGGAGACCCAGGAGTACCGTCTGTATAGAGAAAAGAAAACAGCACCCTGG ATTACACGGATCCCACAGGAGATTGTGAAGAAGGGCCAGTTCCCCATCCCATCCATCACCTGGGAACAC ACAGGGCGGTATCGCTGTTTCTACGGTAGCCACACTGCAGGCTGGTCAGAGCCCAGTGACCCCCTGGAG CTGGTGGTGACAGGAGCCTACATCAAACCCACCCTCTCAGCTCTACCCAGCCCTGTGGTGACCTCAGGA GGGAACGTGACCCTCCATTGTGTCTCACAGGTGGCATTTGGCAGCTTCATTCTGTGTAAGGAAGGAGAA GATGAACACCCACAATGCCTGAACTCACAGCCCCGTACCCATGGGTGGTCCCGGGCCATCTTCTCTGTG GGCCCCGTGAGCCCGAGTCGCAGGTGGTCGTACAGGTGCTATGCTTATGACTCGAACTCTCCCCATGTG TGGTCTCTACCCAGTGATCTCCTGGAGCTCCTGGTCCTAGGTGTTTCTAAGAAGCCATCACTCTCAGTG CAGCCAGGTCCTATAGTGGCCCCTGGGGAGAGCCTGACCCTCCAGTGTGTTTCTGATGTCAGCTACGAC AGATTTGTTCTGTATAAGGAGGGAGAACGTGACTTCCTCCAGCTCCCTGGCCCACAGCCCCAGGCTGGG CTCTCCCAGGCCAACTTCACCCTGGGCCCTGTGAGCCGCTCCTACGGGGGCCAGTACAGATGCTCCGGT GCATACAACCTCTCCTCCGAGTGGTCGGCCCCCAGCGACCCCCTGGACATCCTGATCGCAGGACAGTTC CGTGGCAGACCCTTCATCTCGGTGCATCCGGGCCCCACGGTGGCCTCAGGAGAGAACGTGACCCTGCTG TGTCAGTCATGGGGGCCGTTCCACACTTTCCTTCTGACCAAGGCGGGAGCAGCTGATGCCCCCCTCCGT CTCAGATCAATACACGAATATCCTAAGTACCAGGCTGAATTCCCTATGAGTCCTGTGACCTCAGCCCAC TCGGGGACCTACAGGTGCTACGGCTCACTCAGCTCCAACCCCTACCTGCTGTCTCACCCCAGTGACTCC CTGGAGCTCATGGTCTCAGGAGCAGCTGAGACCCTCAGCCCACCACAAAACAAGTCCGATTCCAAGGCT GGTGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278319
Insert Size	1320 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278319.1
RefSeq Size	1821 bp
RefSeq ORF	1320 bp
Locus ID	11024
UniProt ID	O75019
MW	47.9 kDa
Gene Summary	This gene encodes an activating member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein is predominantly expressed in B cells, interacts with major histocompatibility complex class I ligands, and contributes to the regulation of immune responses. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013] Transcript Variant: This variant (3) has a shorter 5' UTR, lacks several exons and its 3'-terminal exon extends past a splice site that is used in variant 1. The encoded isoform (3) has a shorter and distinct C-terminus, compared to isoform 1. Isoform 3 lacks the transmembrane domain found in isoform 1 and is suspected to be soluble (PMID: 19658091).

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