FMO1 (NM_001282694) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336300 representing NM_001282694. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCAAGCGAGTTGCCATTGTGGGAGCTGGGGTCAGCGGCCTGGCCTCCATCAAGTGCTGTCTGGAA GAAGGACTGGAGCCCACCTGCTTTGAGAGGAGCGATGACCTTGGGGGGCTGTGGAGATTCACCACCAAA GTCTGCAGTGTAACAAAATGCTCAGATTCTGCTGTCTCTGGCCAATGGGAGGTGGTCACTATGCATGAA GAGAAGCAAGAGTCAGCCATCTTTGATGCTGTCATGGTCTGCACTGGCTTTCTTACTAATCCTTATTTG CCACTGGATTCCTTTCCAGGTATTAATGCCTTTAAAGGCCAGTACTTTCATAGCCGGCAATATAAGCAT CCAGATATATTTAAGGACAAGAGAGTCCTTGTGATTGGAATGGGAAATTCTGGCACAGACATTGCTGTG GAGGCCAGCCACCTGGCGGAAAAGGTGTTCCTCAGCACCACCGGAGGGGGATGGGTGATCAGCCGAATC TTTGACTCGGGCTACCCATGGGACATGGTGTTCATGACACGCTTTCAGAACATGTTGAGAAATTCCCTC CCAACCCCAATTGTGACTTGGTTGATGGAGCGAAAGATAAACAACTGGCTCAATCATGCAAATTACGGC TTAATACCAGAAGACAGGACTCAGCTGAAAGAGTTTGTGCTAAATGATGAGCTCCCAGGACGCATCATC ACTGGGAAAGTGTTCATCAGGCCAAGCATAAAAGAGGTAAAGGAAAACTCTGTCATATTTAACAATACT TCAAAGGAAGAGCCTATTGACATCATTGTCTTTGCCACTGGATACACATTTGCTTTCCCCTTCCTTGAT GAGTCTGTAGTGAAAGTTGAAGATGGCCAGGCCTCACTGTACAAGTATATCTTCCCTGCACATCTGCAA AAGCCAACCCTGGCCATTATTGGCCTCATCAAACCCTTGGGCTCCATGATACCTACAGGAGAAACACAA GCTCGGTGGGCTGTTCGAGTCCTGAAAGGTGTAAATAAGTTACCACCACCAAGTGTCATGATAGAGGAA ATTAATGCAAGGAAAGAAAACAAGCCCAGTTGGTTTGGCTTGTGCTACTGCAAGGCTTTACAATCAGAT TATATCACATACATAGATGAACTCCTGACCTATATCAATGCAAAACCCAACCTGTTCTCTATGCTCCTA ACGGATCCACATCTGGCTCTGACCGTCTTCTTTGGCCCATGCTCACCATACCAGTTCCGCTTGACTGGC CCAGGAAAATGGGAAGGAGCCAGAAATGCCATCATGACCCAGTGGGACCGAACATTCAAGGTCATCAAA GCTCGAGTTGTACAAGAGTCTCCATCTCCCTTTGAAAGTTTTCTTAAAGTCTTTAGCTTTCTGGCTTTG CTTGTGGCTATTTTTCTGATTTTCCTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282694
Insert Size	1410 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282694.1
RefSeq Size	2003 bp
RefSeq ORF	1410 bp
Locus ID	2326
UniProt ID	Q01740
Protein Families	Druggable Genome
Protein Pathways	Drug metabolism - cytochrome P450
MW	52.8 kDa
Gene Summary	Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (c) is shorter at the N-terminus and lacks an alternate internal segment compared to isoform a.

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