NIPA (ZC3HC1) (NM_001282190) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HSPC216; NIPA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336376 representing NM_001282190. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGGGGATTGCCCCGGAAGAGGGAGGCGTGGACGCAACCACACCCGCTTGAGGCTCTCTATGAATCT CTGAGAGTCTTAGAGAAGGACACGTCTGCCACATCCCAGTCAGTTAATGGATCACCCCAAGCGGAACAA CCTTCATTGGAATCTACAAGCAAAGAAGCCTTCTTTAGCAGAGTGGAAACATTTTCTTCTTTGAAATGG GCAGGTAAGCCCTTTGAGCTGTCTCCACTCGTCTGTGCAAAATATGGCTGGGTCACAGTGGAATGTGAT ATGCTCAAGTGCTCTAGCTGTCAAGCTTTTCTCTGTGCCAGTTTACAACCAGCTTTTGACTTTGACAGA TATAAGCAACGATGTGCTGAGCTGAAGAAAGCCTTGTGTACTGCCCATGAGAAGTTCTGTTTCTGGCCA GACAGCCCATCCCCAGACCGATTTGGGATGTTGCCCCTGGATGAGCCTGCTATTCTTGTTAGTGAATTC CTAGATCGTTTTCAAAGCCTTTGTCACTTGGACCTCCAGCTTCCTTCCCTAAGGCCGGAGGACTTGAAA ACTATGTGCTTGACAGAAGACAAGATCAGTCTTCTCCTACACTTGCTTGAAGATGAACTTGATCACCGA ACTGATGAGAGAAAAACTACAATCAAATTAGGCTCAGACATCCAAGTCCACGTCACTGCCTGTATTCTC TCTGTGTGTGGCTGGGCGTGTAGTTCCTCTTTGGAATCCATGCAGCTCTCCCTGATAACATGTTCGCAA TGTATGAGGAAGGTGGGGCTCTGGGGCTTCCAGCAGATTGAATCGTCCATGACTGACCTGGATGCATCC TTTGGCCTGACCAGCTCCCCAATCCCAGGCCTTGAGGGGCGACCAGAGCGCTTACCTCTGGTGCCTGAA TCTCCTCGGAGGATGATGACCCGGAGCCAGGATGCCACTTTCTCCCCAGGCTCAGAGCAGGCTGAAAAG AGCCCTGGTCCCATTGTCTCTCGAACTCGGAGCTGGGACTCTTCCAGTCCTGTTGACCGTCCTGAGCCA GAGGCTGCTAGCCCCACCACCAGAACTCGCCCAGTGACCCGAAGCATGGGAACAGGAGACACCCCTGGC CTGGAGGTACCATCTAGCCCTCTGCGGAAAGCCAAGCGAGCTCGCCTCTGCTCCTCCAGCAGTTCGGAC ACATCTTCCCGAAGCTTCTTTGATCCCACCTCTCAGCATAGAGACTGGTGCCCTTGGGTGAATATCACA CTTGGCAAAGAAAGCAGGGAGAATGGTGGAACTGAACCAGATGCCAGCGCCCCAGCAGAGCCAGGCTGG AAAGCAGTGCTGACCATCCTCTTGGCGCACAAACAGTCTAGCCAGCCAGCTGAAACGGACTCCATGAGT CTCTCTGAGAAATCAAGGAAAGTATTCCGAATATTTCGGCAGTGGGAATCTCTGTGCTCATGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282190
Insert Size	1446 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282190.1
RefSeq Size	2049 bp
RefSeq ORF	1446 bp
Locus ID	51530
UniProt ID	Q86WB0
Protein Families	Druggable Genome
MW	53.6 kDa
Gene Summary	This gene encodes an F-box-containing protein that is a component of an SCF-type E3 ubiquitin ligase complex that regulates the onset of cell division. The G2/M transition in the cell cycle requires the interaction of the proteins cyclin B1 and cyclin-dependent kinase 1. The activated ubiquitin ligase complex targets the protein cyclin B1 for degradation, preventing this transition to mitosis. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (2) differs in the 5' UTR, contains an alternate exon in the 5' coding region, and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.

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