CEECAM1 (CERCAM) (NM_001286760) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CEECAM1; GLT25D3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336576 representing NM_001286760. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGCAGGAGTGGCTGGCGGCTGTGGGCGATGACTATGCTGCTGTGGTCTGGAGGCCTGAGGGCGAG CCCAGGTTCTACCCAGATGAAGAGGGTCCCAAGCACTGGACCAAAGAAAGGCACCAGTTTCTGATGGAG CTGAAGCAGGAAGCCCTCACCTTTGCCAGGAACTGGGGGGCCGACTATATCCTGTTTGCAGACACAGAC AACATTCTGACCAACAATCAGACTCTGCGGCTTCTCATGGGGCAGGGGCTTCCAGTGGTGGCCCCAATG CTGGACTCCCAGACCTACTACTCCAACTTCTGGTGTGGGATCACCCCCCAGGGCTACTACCGCCGCACA GCCGAGTACTTCCCCACCAAGAACCGCCAGCGCCGGGGCTGCTTCCGTGTCCCCATGGTCCACTCCACC TTCCTTGCATCCCTGCGGGCTGAAGGGGCAGACCAGCTTGCTTTCTACCCGCCACATCCCAACTACACT TGGCCTTTCGACGACATCATCGTCTTCGCCTATGCCTGCCAGGCTGCTGGGGTCTCCGTCCACGTGTGC AATGAGCACCGTTATGGGTACATGAATGTGCCGGTGAAATCCCACCAGGGGCTGGAAGACGAGAGGGTC AACTTCATCCACCTGATCTTAGAAGCACTAGTGGACGGCCCCCGCATGCAGGCCTCAGCTCATGTGACT CGGCCCTCTAAGAGGCCCAGCAAGATAGGGTTTGACGAGGTCTTTGTCATCAGCCTGGCTCGCAGGCCT GACCGTCGGGAACGCATGCTCGCCTCGCTCTGGGAGATGGAGATCTCTGGGAGGGTGGTGGACGCTGTG GATGGCTGGATGCTCAACAGCAGTGCCATCAGGAACCTCGGCGTAGACCTGCTCCCGGGCTACCAGGAC CCTTACTCGGGCCGCACTCTGACCAAGGGCGAGGTGGGCTGCTTCCTCAGCCATTACTCCATCTGGGAA GAGGTGGTTGCCAGGGGCCTGGCCCGGGTCCTGGTGTTTGAGGATGACGTGCGCTTTGAGAGCAACTTC AGGGGGCGGCTGGAGCGGCTGATGGAGGATGTGGAGGCAGAGAAACTGTCTTGGGACCTGATCTACCTC GGACGGAAGCAGGTGAACCCTGAGAAGGAGACGGCCGTGGAGGGGCTGCCGGGCCTGGTGGTGGCTGGG TACTCCTACTGGACGCTGGCCTATGCCCTGCGTCTGGCGGGTGCCCGCAAGCTGCTGGCCTCACAGCCT CTGCGCCGCATGCTGCCCGTGGACGAGTTCCTGCCCATCATGTTCGACCAGCACCCCAACGAGCAGTAC AAGGCACACTTCTGGCCACGGGACCTGGTGGCCTTCTCCGCCCAGCCCCTGCTCGCTGCCCCTACCCAC TATGCCGGGGACGCCGAGTGGCTCAGTGACACGGAGACATCCTCTCCATGGGATGATGACAGCGGCCGC CTCATCAGCTGGAGCGGCTCCCAAAAGACCCTGCGCAGCCCCCGCCTGGACCTGACTGGCAGCAGCGGG CACAGCCTCCAACCCCAGCCCCGAGATGAGCTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286760
Insert Size	1554 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286760.1
RefSeq Size	2238 bp
RefSeq ORF	1554 bp
Locus ID	51148
UniProt ID	Q5T4B2
MW	59.1 kDa
Gene Summary	Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Does not express any beta-galactosyltransferase activity in vitro.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation from a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a.

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