AGAP8 (AGAP4) (NM_001291379) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AGAP-4; AGAP-8; AGAP8; CTGLF1; CTGLF5; MRIP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336862 representing NM_001291379. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTTTGGAGTTTAACCCTTCTGCCAATCCAGAGGCAAGCACAATATTCCAGAGGAACTCTCAAACAG ATGGTATCTACTGTGCGTTTCAGTCAACAATACAGCTTGTGTTCGACAATATTCCTTGATGACAGCACA GCCATCCAGCATTATCTTACAATGACAATAATATCTGTGACCTTGGAGATACCTCATCATATCACACAA AGAGATGCAGATAGATCTTTGAGCATACCTGATGAACAGTTACACTCATTTGCGGTTTCCACCGTGCAC ATTATGAAGAAAAGAAATGGAGGTGGGAGTTTAAATAACTATTCCTCCTCCATTCCATCGACTCCCAGC ACCAGCCAGGAGGACCCTCAGTTCAGTGTTCCTCCCACTGCCAACACACCCACCCCCGTTTGCAAGCGG TCCATGCGCTGGTCCAACCTGTTTACATCTGAGAAAGGGAGTGACCCAGACAAAGAGAGGAAAGCCCCG GAGAATCATGCTGACACCATCGGGAGCGGCAGAGCCATCCCCATTAAACAGGGCATGCTCTTAAAGCGA AGTGGGAAATGGCTGAAGACATGGAAAAAGAAATACGTCACCCTGTGTTCCAATGGCGTGCTCACCTAT TATTCAAGCTTAGGTGATTATATGAAGAATATTCATAAAAAAGAGATTGACCTTCAGACATCTACCATC AAAGTCCCAGGAAAGTGGCCATCCCTAGCCACATCGGCCTGCACACCCATCTCCACCTCTAAAAGCAAT GGCCTATCCAAGGACATGGACACCGGGCTGGGTGACTCCATATGCTTCAGCCCCAGTATCTCCAGCACC ACCAGCCCCAAGCTCAACCCGCCCCCCTCTCCTCATGCCAATAAAAAGAAACACCTAAAGAAGAAAAGC ACCAACAACTTTATGATTGTGTCTGCCACTGGCCAAACGTGGCACTTTGAAGCCACGACGTATGAGGAG CGGGATGCCTGGGTCCAAGCCATCCAGAGCCAGATCCTGGCCAGCCTGCAGTCATGCGAGAGCAGTAAA AGCAAGTCCCAGCTGACCAGCCAGAGCAAGGCCATGGCCCTGCAGTCGATCCAAAACATGCGTGGGAAC GCCCACTGTGTGGACTGTGAGACCCAGAATCCTAAGTGGGCCAGTTTGAACTTGGGAGTCCTCATGTGT ATTGAATGCTCAGGAATCCACCGCAGTCTTGGCACCCGCCTTTCCCGTGTGCGATCTCTGGAGCTGGAT GACTGGCCAGTTGAGCTCAGGAAGGTTATGTCATCTATTGGCAATGACCTAGCCAACAGCATCTGGGAA GGGAGCAGCCAGGGGCAGACAAAACCCTCAGAAAAGTCCACGAGGGAAGAGAAGGAACGGTGGATCCGT TCCAAATATGAGGAGAAGCTCTTTCTGGCCCCACTACCCTGCACTGAGCTGTCCCTGGGCCAGCAGCTG CTGCGGGCCACCGCTGATGAGGACCTGCAGACAGCCATCCTGCTGCTGGCACATGGCTCCCGTGAGGAG GTGAACGAGACCTGTGGGGAGGGAGACGGCTGCACGGCGCTCCATCTGGCCTGCCGCAAGGGGAATGTG GTCCTGGCGCAGCTCCTGATCTGGTACGGGGTGGACGTCATGGCCCGAGATGCCCACGGGAACACAGCG CTGACCTACGCCCGGCAGGCCTCCAGCCAGGAGTGCATCAACGTGCTTCTGCAGTACGGCTGCCCCGAC AAGTGTGTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291379
Insert Size	1737 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291379.1
RefSeq Size	2712 bp
RefSeq ORF	1737 bp
Locus ID	119016
MW	64 kDa
Gene Summary	Putative GTPase-activating protein.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, which results in a frameshift, compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.