SCFD1 (NM_001283032) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C14orf163; RA410; SLY1; SLY1P; STXBP1L2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336874 representing NM_001283032. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGCCTCATATTAAAAACAGCACAGGAGAACCAGTATGGAAGGCTTTTACACTCTGATCGAGATCCT ATTCCAGATGTTCCTGCAGTATACTTTGTAATGCCAACTGAAGAAAATATTGACAGAATGTGCCAGGAT CTTCGAAATCAACTATATGAATCATATTATTTAAATTTTATTTCTGCTATTTCAAGAAGTAAACTGGAA GATATTGCAAATGCAGCGTTAGCAGCTAGTGCAGTAACACAAGTAGCCAAGGTTTTTGACCAATATCTC AATTTTATTACTTTGGAAGATGATATGTTTGTATTATGTAATCAAAATAAGGAGCTTGTTTCATATCGT GCCATTAACAGGCCAGATATCACAGACACGGAAATGGAAACTGTTATGGACACTATAGTTGACAGCCTC TTCTGCTTTTTTGTTACTCTGGGTGCTGTTCCTATAATCAGATGTTCAAGAGGAACAGCAGCAGAAATG GTAGCAGTGAAACTAGACAAGAAACTTCGAGAAAATCTAAGAGATGCAAGAAACAGTCTTTTTACAGGT GATACACTTGGAGCTGGCCAATTCAGCTTCCAGAGGCCCTTATTAGTCCTTGTTGACAGAAACATAGAT TTGGCAACTCCTTTACATCATACTTGGACATATCAAGCATTGGTGCACGATGTACTGGATTTCCATTTA AACAGGGTTAATTTGGAAGAATCTTCAGGAGTGGAAAACTCTCCAGCTGGTGCTAGACCAAAGAGAAAA AACAAGAAGTCTTATGATTTAACTCCGGTTGATAAATTTTGGCAAAAACATAAAGGAAGTCCATTCCCA GAAGTTGCAGAATCAGTTCAGCAAGAACTAGAATCTTACAGAGCACAGGAAGATGAGGTCAAACGACTT AAAAGCATTATGGGACTAGAAGGGGAAGATGAAGGAGCCATAAGTATGCTTTCTGACAATACCGCTAAG CTAACATCAGCTGTTAGTTCTTTGCCAGAACTCCTTGAGAAAAAAAGACTTATTGATCTCCATACAAAT GTTGCCACTGCTGTTTTAGAACATATAAAGGCAAGAAAATTGGATGTATATTTTGAATATGAAGAAAAA ATAATGAGCAAAACTACTCTGGATAAATCTCTTCTAGATATAATATCAGACCCTGATGCAGGAACTCCA GAAGATAAAATGAGGTTGTTTCTTATCTATTATATAAGCACACAGCAAGCACCTTCTGAGGCTGATTTG GAGCAATATAAAAAAGCTTTAACTGATGCAGGATGCAACCTTAATCCTTTACAATATATCAAACAGTGG AAGGCTTTTACCAAGATGGCCTCAGCTCCGGCCAGCTATGGCAGCACTACCACTAAACCAATGGGTCTT TTATCACGAGTCATGAATACAGGATCACAGTTTGTGATGGAAGGAGTGAAGAACCTGGTTTTGAAACAG CAAAATCTACCTGTTACTCGTATTTTGGACAATCTTATGGAGATGAAGTCAAACCCCGAAACTGATGAC TATAGATATTTTGATCCCAAAATGCTGCGGGGCAATGACAGCTCAGTTCCCAGAAATAAAAATCCATTC CAAGAGGCCATTGTTTTTGTGGTGGGAGGAGGCAACTACATTGAATATCAGAATCTTGTTGACTACATA AAGGGGAAACAAGGCAAACACATTTTATATGGCTGCAGTGAGCTTTTTAATGCTACACAGTTCATAAAA CAGTTGTCACAACTTGGACAAAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001283032
Insert Size	1752 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001283032.1
RefSeq Size	2144 bp
RefSeq ORF	1752 bp
Locus ID	23256
UniProt ID	Q8WVM8
MW	66 kDa
Gene Summary	Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) lacks an exon in the 5' region and initiates translation at an alternate downstream start codon, compared to variant 1. The encoded isoform (e) is shorter and has a distinct N-terminus, compared to isoform a.

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