ZNF131 (NM_001297548) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	pHZ-10; ZBTB35
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337024 representing NM_001297548. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGCTGAAGAGACGATGGAATGCCTTCAGGAGTTCCCTGAACATCATAAAATGATCCTCGACCGA TTGAATGAACAGCGAGAGCAGGACCGGTTTACTGACATCACCCTAATTGTCGACGGACACCATTTTAAG GCTCACAAGGCTGTTTTGGCTGCTTGTAGTAAGTTCTTCTACAAATTCTTTCAGGAGTTTACCCAAGAA CCATTGGTGGAGATAGAAGGTGTTAGTAAAATGGCCTTTCGCCATTTAATTGAGTTCACATATACAGCA AAATTAATGATACAAGGAGAAGAAGAAGCCAATGATGTATGGAAAGCAGCAGAGTTTCTACAAATGCTA GAAGCTATCAAAGCCCTTGAAGTCAGGAACAAAGAAAACTCAGCTCCCTTAGAGGAAAATACCACAGGA AAAAATGAGGCCAAAAAAAGGAAGATTGCAGAAACTTCAAATGTTATCACTGAGTCATTGCCATCTGCA GAATCAGAACCTGTTGAAATTGAGGTAGAGATTGCCGAAGGCACCATTGAAGTGGAAGATGAAGGCATC GAAACATTAGAGGAAGTGGCTTCTGCCAAGCAGTCCGTAAAGTACATACAGAGCACAGGTTCCTCTGAT GATTCTGCTCTAGCACTGTTGGCAGATATTACCAGCAAGTACCGTCAAGGTGACAGAAAAGGGCAGATT AAAGAAGATGGCTGTCCATCTGACCCCACGAGCAAACAGGTAGAAGGTATTGAAATTGTGGAACTTCAG CTGTCACATGTGAAGGACTTGTTCCATTGTGAGAAATGTAACCGTTCATTTAAATTGTTTTACCATTTT AAGGAGCACATGAAATCACACTCCACTGAGAGTTTCAAGTGTGAAATATGCAATAAACGATATCTTCGA GAGAGCGCATGGAAACAGCACCTAAATTGTTACCACCTTGAAGAAGGTGGAGTCAGTAAGAAGCAAAGA ACTGGGAAAAAAATTCATGTATGTCAGTACTGTGAGAAACAGTTTGACCATTTTGGACATTTTAAAGAA CATCTTCGAAAACATACAGGTGAAAAACCTTTTGAATGTCCAAATTGTCATGAACGATTTGCTAGAAAT AGCACTCTGAAATGTCACCTCACTGCATGCCAAACTGGAGTAGGGGCAAAAAAAGGAAGGAAGAAGCTC TACGAATGCCAGGTCTGCAACAGTGTGTTTAACAGCTGGGACCAGTTCAAAGATCACTTGGTAATACAC ACTGGAGATAAACCCAACCATTGTACTTTATGTGATTTGTGGTTTATGCAAGGAAATGAATTAAGGAGG CATCTCAGTGATGCTCACAATATTTCAGAGCGTCTAGTAACGGAAGAAGTTCTTTCAGTAGAAACACGT GTGCAAACTGAACCTGTAACATCAATGACTATTATAGAACAAGTTGGGAAGGTGCATGTGCTACCATTG CTTCAGGTTCAGGTGGATTCAGCACAAGTGACTGTGGAACAAGTCCATCCAGATCTGCTCCAGGACAGC CAGGTGCACGATTCACACATGAGTGAGCTTCCAGAGCAGGTCCAAGTGAGTTATCTAGAAGTGGGCCGA ATTCAGACTGAAGAAGGTACTGAAGTACATGTAGAGGAGCTGCATGTTGAACGGGTCAATCAAATGCCA GTGGAAGTACAAACTGAACTTCTAGAAGCAGATTTGGACCACGTGACCCCAGAAATCATGAACCAAGAG GAGAGAGAGTCTAGCCAAGCAGATGCTGCTGAGGCTGCCAGGGAAGATCACGAAGATGCTGAGGATTTA GAGACCAAGCCAACAGTGGATTCTGAAGCAGAAAAGGCAGAGAATGAGGACAGAACAGCTCTGCCAGTT TTAGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297548
Insert Size	1872 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297548.1
RefSeq Size	3109 bp
RefSeq ORF	1872 bp
Locus ID	7690
UniProt ID	P52739
Protein Families	Transcription Factors
MW	71.4 kDa
Gene Summary	Plays a role during development and organogenesis as well as in the function of the adult central nervous system (By similarity). May be involved in transcriptional regulation as a repressor of ESR1/ER-alpha signaling.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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