IMPG1 (NM_001282368) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GP147; IPM150; SPACR; VMD4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282368, the custom clone sequence may differ by one or more nucleotides ATGTATTTGGAAACTAGAAGAGCTATTTTTGTTTTTTGGATTTTTCTCCAAGTTCAAGGAACCAAAGTGT GTCAGGAAGCAGTATGGGAAGCATATCGGATCTTTCTGGATCGCATCCCTGACACAGGGGAATATCAGGA CTGGGTCAGCATCTGCCAGCAGGAGACCTTCTGCCTCTTTGACATTGGAAAAAACTTCAGCAATTCCCAG GAGCACCTGGATCTTCTCCAGCAGAGAATAAAACAGAGAAGTTTCCCTGACAGAAAAGATGAAATATCTG CAGAGAAGACATTGGGAGAGCCTGGTGAAACCATTGTCATTTCAACAGATGTTGCCAACGTCTCACTTGG GCCTTTCCCTCTCACTCCTGATGACACCCTCCTCAATGAAATTCTCGATAATACACTCAACGACACCAAG ATGCCTACAACAGAAAGAGAAACAGAATTCGCTGTGTTGGAGGAGCAGAGGGTGGAGCTCAGCGTCTCTC TGGTAAACCAGAAGTTCAAGGCAGAGCTCGCTGACTCCCAGTCCCCATATTACCAGGAGCTAGCAGGAAA GTCCCAACTTCAGATGCAAAAGATATTTAAGAAACTTCCAGGATTCAAAAAAATCCATGTGTTAGGATTT AGACCAAAGAAAGAAAAAGATGGCTCAAGCTCCACAGAGATGCAACTTACGGCCATCTTTAAGAGACACA GTGCAGAAGCAAAAAGCCCTGCAAGTGACCTCCTGTCTTTTGATTCCAACAAAATTGAAAGTGAGGAAGT CTATCATGGAACCATGGAGGAGGACAAGCAACCAGAAATCTATCTCACAGCTACAGACCTCAAAAGGCTG ATCAGCAAAGCACTAGAGGAAGAACAATCTTTGGATGTGGGGACAATTCAGTTCACTGATGAAATTGCTG GATCACTGCCAGCCTTTGGTCCTGACACCCAATCAGAGCTGCCCACATCTTTTGCTGTTATAACAGAGGA TGCTACTTTGAGTCCAGAACTTCCTCCTGTTGAACCCCAGCTTGAGACAGTGGACGGAGCAGAGCATGGT CTACCTGACACTTCTTGGTCTCCACCTGCTATGGCCTCTACCTCCCTGTCAGAAGCTCCACCTTTCTTTA TGGCATCAAGCATCTTCTCTCTGACTGATCAAGGCACCACAGATACAATGGCCACTGACCAGACAATGCT AGTACCAGGGCTCACCATCCCCACCAGTGATTATTCTGCAATCAGCCAACTGGCTCTGGGAATTTCACAT CCACCTGCATCTTCAGATGACAGCCGATCAAGTGCAGGTGGCGAAGATATGGTCAGACACCTAGATGAAA TGGATCTGTCTGACACTCCTGCCCCATCTGAGGTACCAGAGCTCAGCGAATATGTTTCTGTCCCAGATCA TTTCTTGGAGGATACCACTCCTGTCTCAGCTTTACAGTATATCACCACTAGTTCTATGACCATTGCCCCC AAGGGCCGAGAGCTGGTAGTGTTCTTCAGTCTGCGTGTTGCTAACATGGCCTTCTCCAACGACCTGTTCA ACAAGAGCTCTCTGGAGTACCGAGCTCTGGAGCAACAATTCACACAGCTGCTGGTTCCATATCTACGATC CAATCTTACAGGATTTAAGCAACTTGAAATACTTAACTTCAGAAACGGGAGTGTGATTGTGAATAGCAAA ATGAAGTTTGCTAAGTCAGTGCCGTATAACCTCACCAAGGCTGTGCACGGGGTCTTGGAGGATTTTCGTT CTGCTGCAGCCCAACAACTCCATCTGGAAATAGACAGCTACTCTCTCAACATTGAACCAGCTGATCAAGC AGATCCCTGCAAGTTCCTGGCCTGCGGCGAATTTGCCCAATGTGTAAAGAACGAACGGACTGAGGAAGCG GAGTGTCGCTGCAAACCAGGATATGACAGCCAGGGGAGCCTGGACGGTCTGGAACCAGGCCTCTGTGGCC CTGGCACAAAGGAATGCGAGGTCCTCCAGGGAAAGGGAGCTCCATGCAGGTTGCCAGATCACTCTGAAAA TCAAGCATACAAAACTAGTGTTAAAAAGTTCCAAAATCAACAAAATAACAAGGTAATCAGTAAAAGAAAT TCTGAATTACTGACCGTAGAATATGAAGAATTTAACCATCAAGATTGGGAAGGAAATTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282368
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282368.1, NP_001269297.1
RefSeq Size	3342 bp
RefSeq ORF	2160 bp
Locus ID	3617
UniProt ID	Q17R60
Protein Families	Druggable Genome, Secreted Protein
Gene Summary	This gene encodes a protein that is a major component of the retinal interphotoreceptor matrix. The encoded protein is a proteoglycan that is thought to play a role in maintaining viability of photoreceptor cells and in adhesion of the neural retina to the retinal pigment epithelium. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (2) lacks one in-frame alternate exon compared to variant 1. The encoded isoform (2) is shorter compared to isoform 1.

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