ZNF778 (NM_001201407) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001201407, the custom clone sequence may differ by one or more nucleotides ATGGCAGCCCCTGACCTGGCCCACGGAGGTCATGTTTCTAGGGACTCAGTCTGCCTTCATGAAGAACAGA CACAGGCAGCAGGGATGGTGGCTGGCTGGCTGATAAATTGTTACCAGGACGCGGTGACCTTTGACGACGT GGCTGTGGACTTCACCCAGGAGGAATGGACTTTACTGGACCCATCTCAGAGAGACCTCTACAGAGATGTG ATGCTGGAAAACTACGAGAACCTGGCCTCAGTAGGACATCACCTGTTCCAACCCAGTGTGATCTATTGGC TGGAGCAGGAAGAGGAGTTGAGGGCAGGGCGGAGAGCAGTTCTCCAAGAATGGCGACTTAAAACCAAAGG GCCAGCACTTCGGCAGGATAGATCTTGGTTCAGAGCATCAAATGAGACACAGACGGCAAGAAGCCACAAT GGAGGGCAGCTCTGTGACCGCACGCAGTGTGGAGAAGCTTTCAGTGAACACTCAGGCCTCAGCACACACG TGAGAACTCAAAATACAGGAGACAGTTGTGTGTCTAATCATTATGAAAGGGACTTTTTTATTCCATGCCA GAAAACCTTGTTCAAAATTGGAGAGCAGTTTTCCGTGTTGGGTCAGTGTGGAAAAGCCTTCAGCTCTACT CCAAATGTTGTTTCCCAGCAAGCATGCACTCGGGACAGATCTCTTGACTACAGCAGCTGTGGGGAAGTGT TCCTTAATCAGTCATACCTTCAGGCACGTGCGGGAAGTCACAACGGAGAAGAAACATGGAAATGGAAGCC GTGTGGGAAAGCTCTAACTCACTCCATGGGCTGCGCCACACCTGTTGAAATGCATGCCGTCAGGAATCCC CACGTATGTAGGGAATGTGGGAAGGCCTTTAGGTACACTGCCTACCTTACTGGTCGCGTGCAAGTCCACC CTGGGGAAAAGCCCTGTGAATTGGAAGAATGTGGAAAAGCCTCCCCTGTTTCTTCCAGCCTAACTCAACA TGTAAGAATTCATGCTGCAGAGAAACCCTGTGAATGTAAAGAATGCGGAAAAGCCTTCACTGGACTCTCA GGTCTTTCTAAACACGTCCAAACAGACCCTGGACAGAAGCCCTATGAATGTAAGGACTGTGGGAAAGCCT GCGGTGGGTTTTATCTACTGAATGAGCATGGAAAAACTCACACGAGGGAGAAGCCCTTTGCATGTGTGGT TTGCGGAAAATATTTTAGAAATTCCTCATGCCTTAATAATCATGTTCGAATTCACACTGGAATAAAACCC TATACATGCAGCTACTGTGGGAAGGCCTTCACTGTGCGCTGTGGCCTTACTAGACACGTACGAACACACA CGGGCGAGAAGCCATACACGTGTAAGGACTGCGGGAAAGCCTTCTGTACATCCTCGGGCCTTACTGAGCA TGTAAGGACTCACACTGGAGAGAAACCATATGAATGTAAAGATTGTGGGAAATCCTTCACTGTTTCTTCA AGCCTGACTGAGCACGCGAGAATCCATACCGGAGAGAAACCCTACGAATGTAAGCAGTGTGGCAAAGCCT TCACAGGGCGCTCAGGCCTCACTAAACACATGCGGACACACACCGGGGAGAAGCCCTATGAATGTAAGGA CTGTGGGAAAGCCTACAATAGGGTTTATCTACTGAATGAGCATGTGAAAACTCACACAGAGGAGAAGCCC TTTATATGTACGGTATGCAGGAAATCCTTCAGAAATTCCTCGTGCCTGAATAAGCACATTCAGATTCACA CTGGAATAAAACCTTATGAATGTAAGGACTGTGGGAAAACATTCACTGTTTCTTCGAGCCTAACCGAGCA CATACGAACTCACACTGGAGAGAAACCTTATGAATGTAAAGTATGCGGAAAGGCCTTCACCACATCCTCA CACCTTATCGTGCACATAAGAACCCACACCGGTGAGAAACCCTACATATGTAAGGAGTGTGGGAAAGCCT TTGCTTCCTCCTCACACCTTATCGAACACAGAAGGACTCACACAGGAGAGAAACCTTACATATGTAACGA GTGTGGGAAAGCCTTCCGTGCCTCCTCTCACCTGCATAAACATGGAAGAATTCACACTGGGCAGAAACCC TATAAATGTAAGGAATGTGGGAAAGCATACAATAGGTTTTATCTACTAAAAGAACATTTAAAAACTTACA CTGAAGAGCAGGTTTTTGTATGTAAGGACTGTGGAAAATCTTTTAAGAATTCCTCATGCCTTAACCATCA CACTCAAATTCACACTGATGAGAAACCTTTCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001201407
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001201407.1, NP_001188336.1
RefSeq Size	3608 bp
RefSeq ORF	2274 bp
Locus ID	197320
Protein Families	Transcription Factors
Gene Summary	The protein encoded by this gene is a member of the krueppel C2H2-type zinc-finger protein family, and it contains one KRAB domain and eighteen C2H2-type zinc fingers. This gene is a candidate gene for autism and variable cognitive impairment in the 16q24.3 microdeletion syndrome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2011] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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