SECISBP2 (NM_001282689) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SBP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282689, the custom clone sequence may differ by one or more nucleotides ATGGTTAGAGTCCTCAGAAGCATGTGTCTTCCCCAGCTCTGCAGCCACATACTATCCGTTTGTTCAGGAA CCACCAGTGACAGAAATGTTTACTCAGTGCCTGGCTCCCAGTATCTTTATAACCAACCCAGTTGTTACCG AGGTTTTCAAACAGTGAAGCATCGAAATGAGAACACATGCCCTCTCCCACAAGAAATGAAAGCTCTGTTT AAGAAGAAAACCTATGATGAGAAAAAAACGTATGATCAGCAAAAGTTTGACAGTGAAAGGGCTGATGGAA CTATATCATCTGAGATAAAATCAGCTAGAGGTTCACATCATTTGTCCATTTACGCTGAGAATAGTTTGAA ATCAGATGGTTACCATAAGCGAACAGACAGGAAATCCAGAATCATTGCAAAAAATGTATCTACCTCCAAA CCTGAGTTTGAATTTACCACACTGGACTTTCCTGAACTGCAAGGTGCAGAGAACAATATGTCAGAGATAC AGAAGCAACCCAAGTGGGGACCTGTCCACTCTGTCTCTACCGACATTTCTCTTCTAAGAGAAGTAGTAAA ACCAGCTGCAGTGTTATCAAAGGGTGAAATAGTGGTGAAAAATAACCCAAATGAATCTGTAACTGCTAAT GCCGCTACCAATTCTCCTTCATGTACAAGAGAGTTATCTTGGACACCAATGGGTTATGTTGTTCGACAGA CATTATCTACAGAACTGTCAGCAGCCCCTAAAAATGTTACTTCTATGATAAACTTAAAGACCATTGCTTC ATCAGCAGATCCTAAAAATGTTAGTATACCATCTTCTGAAGCTTTATCTTCGGATCCTTCCTACAACAAA GAAAAACACATTATTCATCCTACCCAAAAGTCTAAAGCATCACAAGGTAGTGACCTTGAACAAAATGAAG CCTCAAGAAAGAATAAGAAAAAGAAAGAAAAATCTACATCAAAATATGAAGTCCTGACAGTTCAAGAGCC TCCAAGGATTGAAGATGCCGAGGAATTTCCCAACCTGGCAGTTGCATCTGAAAGAAGAGACAGAATAGAG ACACCGAAATTTCAATCTAAGCAGCAGCCACAGGATAATTTTAAAAATAATGTAAAGAAGAGCCAGCTTC CAGTGCAGTTGGACTTGGGGGGCATGCTGACAGCCCTGGAGAAGAAGCAGCACTCTCAGCATGCAAAGCA GTCCTCCAAACCAGTGGTAGTCTCAGTTGGAGCAGTGCCAGTCCTTTCCAAAGAATGTGCATCAGGGGAG AGAGGCCGCCGCATGAGTCAAATGAAGACCCCGCACAATCCCTTGGACTCCAGCGCCCCACTGATGAAGA AAGGGAAGCAGAGGGAGATCCCCAAGGCCAAGAAGCCAACCTCACTGAAGAAGATTATTTTGAAAGAACG GCAAGAGAGAAAGCAGCGTCTCCAAGAAAATGCTGTGAGTCCAGCTTTTACCAGTGATGACACACAAGAT GGAGAGAGTGGTGGTGATGACCAGTTTCCCGAGCAGGCAGAGCTGTCAGGGCCAGAGGGGATGGACGAAC TGATCTCCACTCCTTCGGTTGAGGACAAGTCTGAAGAGCCACCAGGCACAGAGCTCCAGAGGGACACAGA GGCCTCCCACCTTGCTCCCAATCACACCACCTTCCCTAAGATCCACAGCCGCAGATTCAGGGATTACTGC AGCCAGATGCTTAGTAAAGAAGTGGATGCTTGTGTTACCGACCTACTCAAAGAACTGGTCCGTTTCCAAG ACCGTATGTACCAGAAAGATCCAGTCAAGGCCAAGACTAAACGTCGACTTGTGTTGGGGTTGAGGGAGGT TCTCAAACACCTGAAGCTCAAAAAACTGAAATGTGTCATTATTTCTCCCAACTGTGAGAAGATACAGTCA AAAGGTGGGCTGGATGACACTTTGCACACAATTATTGATTATGCCTGTGAGCAGAACATTCCCTTTGTGT TTGCTCTCAACCGCAAAGCTCTGGGGCGCAGTTTGAATAAGGCAGTTCCTGTCAGTGTGGTGGGGATCTT CAGCTATGATGGGGCCCAGGATCAGTTCCACAAGATGGTTGAGCTGACAGTGGCGGCCCGACAGGCGTAC AAGACCATGCTGGAGAATGTGCAGCAGGAGCTGGTGGGAGAGCCCAGGCCTCAGGCACCTCCCAGCCTAC CCACACAGGGCCCCAGCTGCCCTGCAGAAGATGGCCCCCCAGCCCTGAAAGAAAAAGAAGAGCCACACTA CATTGAAATCTGGAAAAAACATCTGGAAGCATACAGTGGATGTACCCTGGAGCTAGAAGAATCCTTGGAG GCTTCAACCTCTCAAATGATGAATTTGAATTTATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001282689
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282689.1, NP_001269618.1
RefSeq Size	3414 bp
RefSeq ORF	2346 bp
Locus ID	79048
UniProt ID	Q96T21
Gene Summary	The protein encoded by this gene is one of the essential components of the machinery involved in co-translational insertion of selenocysteine (Sec) into selenoproteins. Sec is encoded by the UGA codon, which normally signals translation termination. The recoding of UGA as Sec codon requires a Sec insertion sequence (SECIS) element; present in the 3' untranslated regions of eukaryotic selenoprotein mRNAs. This protein specifically binds to the SECIS element, which is stimulated by a Sec-specific translation elongation factor. Mutations in this gene have been associated with reduction in enzymatic activity of type II iodothyronine deiodinase (a selenoprotein) and abnormal thyroid hormone metabolism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (3) uses an alternate in-frame acceptor splice site in the 5' coding region, which results in translation initiation from an alternate start codon compared to variant 1. The encoded isoform (3) is shorter, with a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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