TTC7A (NM_001288953) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GIDID; MINAT; TTC7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001288953, the custom clone sequence may differ by one or more nucleotides ATGGTGCCCAGCTCGTCTCGAACTCCTGGGTTCAAGCAATCCTCCCACCACCTCCGCCTCCCAAAGTGCT GGGATTACAGGGATGACTTTGGGAAATTGCTGCTGGCTGAGGCCCTCCTGGAGCAGTGTTTGAAGGAGAA CCATGCCAAAATAAAAGACTCCATGCCTTTGCTGGAGAAGAATGAGCCGAAGATGAGCGAAGCCAAAAAT TATCTAAGCAGTATCCTTAACCATGGGAGGCTCTCGCCACAGTACATGTGTGAGGCCATGCTGATCCTGG GCAAACTGCATTACGTGGAGGGCTCATACCGAGATGCCATCAGCATGTACGCACGGGCCGGGATTGATGA CATGTCCATGGAGAACAAGCCCCTGTATCAGATGCGGCTGCTGTCGGAGGCTTTTGTCATCAAAGGCCTC TCTCTGGAACGCCTACCCAACTCCATCGCCTCCCGCTTCCGCCTGACAGAGAGGGAGGAGGAAGTGATCA CCTGTTTTGAGAGGGCCTCCTGGATCGCTCAGGTGTTCCTGCAGGAATTGGAGAAGACCACAAATAACAG CACGTCGAGGCATCTGAAAGGCTGTCACCCGCTTGACTATGAGCTCACCTACTTCCTGGAAGCTGCCCTC CAGAGCGCCTATGTGAAAAACCTGAAGAAGGGGAACATCGTGAAGGGCATGAGAGAGCTCCGGGAGGTGC TGCGGACTGTGGAGACCAAAGCAACTCAGAACTTCAAAGTGATGGCGGCCAAGCACCTGGCGGGGGTCCT GCTGCACTCCCTGAGTGAGGAGTGCTACTGGAGCCCCCTGTCCCACCCTCTGCCTGAGTTCATGGGCAAG GAGGAGAGTTCTTTCGCCACTCAGGCCCTGCGGAAACCTCACCTCTATGAAGGAGACAACCTCTACTGCC CCAAGGACAACATCGAGGAAGCCCTCCTGCTCCTCCTCATCAGCGAATCCATGGCAACTCGAGATGTGGT GCTGAGCCGGGTGCCGGAGCAGGAGGAGGACCGGACAGTGAGCTTGCAGAATGCCGCAGCCATCTATGAC CTCCTGAGCATCACGTTGGGCAGAAGGGGACAGTACGTCATGCTCTCGGAGTGCCTGGAGCGAGCCATGA AGTTTGCGTTTGGAGAATTTCACCTTTGGTACCAGGTGGCCCTCTCCATGGTGGCTTGTGGGAAGTCAGC CTACGCTGTGTCCCTGCTGCGGGAGTGTGTGAAGTTGCGGCCCTCGGACCCCACCGTGCCCCTGATGGCC GCGAAGGTCTGCATCGGGTCCCTTCGCTGGCTAGAGGAAGCAGAGCACTTTGCCATGATGGTGATCAGCC TCGGAGAGGAAGCCGGGGAGTTCCTCCCCAAGGGCTACCTGGCTCTGGGTCTCACCTATAGCCTGCAGGC CACCGACGCCACCCTGAAGTCCAAGCAAGATGAATTGCACCGGAAGGCACTGCAGACGCTGGAGAGGGCT CAGCAGCTGGCGCCCAGTGACCCCCAGGTCATCCTCTATGTCTCGCTGCAGCTGGCCCTCGTCCGACAGA TCTCCAGTGCCATGGAGCAGCTGCAGGAGGCCCTGAAGGTACGCAAGGATGATGCCCACGCCCTCCACCT GCTGGCACTGCTCTTCTCTGCCCAGAAGCACCACCAGCATGCCCTGGATGTTGTCAACATGGCCATCACC GAGCACCCTGAGAACTTCAACCTGATGTTCACCAAGGTGAAGCTGGAGCAGGTGCTGAAAGGCCCAGAGG AAGCCCTCGTGACCTGCAGACAAGTGCTGAGGCTGTGGCAGACCCTGTACAGCTTCTCCCAGCTGGGAGG CCTAGAAAAGGATGGCAGCTTCGGTGAGGGCCTCACCATGAAGAAGCAGAGTGGCATGCACCTGACTTTG CCTGATGCCCATGATGCAGACTCTGGCTCCCGGCGGGCTTCGTCCATCGCCGCCTCCCGGCTGGAGGAGG CCATGTCAGAGCTGACTATGCCCTCTTCGGTCCTGAAGCAGGGCCCCATGCAGCTGTGGACCACGCTGGA ACAGATCTGGCTGCAGGCTGCTGAGCTGTTCATGGAGCAGCAGCACCTCAAGGAAGCAGGTTTCTGCATC CAGGAGGCGGCGGGCCTCTTCCCCACTTCTCACTCAGTACTCTATATGCGGGGCCGGCTGGCTGAGGTGA AGGGCAACCTGGAGGAGGCCAAGCAGCTGTACAAGGAGGCGCTCACGGTGAACCCAGATGGCGTGCGCAT CATGCATAGCCTGGGTCTGATGCTGAGTCGGCTGGGCCACAAGAGCTTGGCCCAGAAGGTGCTTCGTGAT GCCGTGGAGAGGCAGAGTACGTGCCACGAGGCGTGGCAGGGCCTGGGCGAGGTGCTGCAGGCCCAGGGCC AGAACGAGGCTGCCGTTGACTGCTTCCTCACCGCCCTTGAGCTGGAGGCCAGCAGCCCTGTACTGCCCTT CTCCATCATCCCCAGAGAGCTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001288953
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288953.1, NP_001275882.1
RefSeq Size	5166 bp
RefSeq ORF	2475 bp
Locus ID	57217
UniProt ID	Q9ULT0
Gene Summary	This gene encodes a protein containing tetratricopeptide repeats. Mutations in this gene disrupt intestinal development and can cause early onset inflammatory bowel disease and intestinal atresia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (3) contains an alternate 5' exon structure and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 3, which is shorter and has a distinct N-terminus, compared to isoform 1.

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