ARHGAP11A (NM_001286480) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GAP (1-12)
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286480, the custom clone sequence may differ by one or more nucleotides ATGGACAGCAGCAATCTTGCAGTAATATTTGCACCGAATCTTCTTCAGACAAGTGAAGGACATGAAAAGA TGTCTTCTAACACAGAAAAGAAGCTACGATTACAGGCTGCAGTAGTACAGACTCTTATCGATTATGCATC AGATATTGGGCGTGTACCAGATTTTATCCTGGAAAAGATACCAGCCATGTTGGGTATTGATGGTCTCTGT GCTACTCCATCACTGGAAGGCTTTGAAGAAGGTGAATATGAAACTCCTGGTGAATATAAGAGAAAGAGAA GACAAAGTGTAGGAGATTTTGTTAGTGGAGCACTAAATAAATTTAAACCTAACAGAACACCTTCTATTAC ACCTCAAGAAGAAAGAATTGCCCAGCTATCTGAATCACCAGTGATTCTTACACCAAATGCTAAGCGTACA TTGCCAGTAGATTCTTCTCATGGTTTCTCAAGTAAGAAAAGGAAGTCCATCAAGCACAATTTTAACTTTG AGCTGTTGCCAAGTAATCTCTTCAATAGCAGTTCTACACCGGTATCAGTTCACATCGATACAAGCTCAGA AGGGTCATCTCAGAGTTCACTCTCTCCTGTACTCATTGGTGGAAACCATTTGATCACTGCAGGTGTGCCA AGGCGAAGTAAAAGAATTGCAGGCAAAAAAGTTTGCAGAGTGGAATCAGGAAAAGCAGGCTGCTTTTCTC CTAAAATCAGCCATAAAGAAAAGGTTCGAAGATCTCTGCGTTTGAAATTCAATCTAGGGAAAAATGGCAG AGAAGTAAATGGATGTTCTGGTGTCAATAGATATGAAAGTGTTGGTTGGCGACTTGCAAATCAACAAAGT TTAAAAAATCGAATTGAATCTGTAAAAACAGGTTTGCTTTTTAGCCCAGATGTTGATGAAAAGTTACCAA AGAAAGGTTCAGAAAAGATCAGTAAGTCTGAGGAAACCTTACTAACTCCAGAGCGACTAGTTGGAACAAA TTACCGGATGTCTTGGACAGGACCTAATAATTCAAGTTTTCAAGAAGTAGATGCAAATGAAGCTTCTTCA ATGGTGGAAAATCTTGAGGTAGAAAACTCTTTGGAGCCTGATATTATGGTAGAAAAGTCACCTGCTACTT CATGTGAACTCACCCCTTCCAATTTAAACAATAAGCATAATAGCAACATAACAAGTAGCCCTCTTAGCGG GGATGAAAATAACATGACCAAAGAGACTTTGGTGAAAGTTCAAAAAGCGTTTTCTGAATCTGGAAGTAAT CTTCACGCATTGATGAATCAGAGGCAGTCATCAGTAACTAATGTGGGGAAAGTAAAATTAACTGAACCAT CTTATTTAGAAGATAGCCCAGAGGAAAATCTATTTGAAACTAATGATTTGACTATAGTAGAATCAAAGGA GAAATATGAACACCACACTGGTAAAGGTGAAAAATGTTTTTCAGAGAGGGACTTTTCACCCCTTCAAACT CAAACATTTAATAGAGAAACAACTATAAAATGTTATTCAACTCAGATGAAGATGGAACATGAAAAAGACA TTCATTCAAATATGCCAAAAGATTATTTAAGCAAGCAAGAATTCTCCAGTGATGAAGAAATAAAGAAACA GCAGTCCCCAAAGGATAAACTAAATAATAAATTAAAAGAGAATGAGAATATGATGGAAGGTAACTTACCG AAGTGTGCAGCACATAGCAAGGACGAGGCTAGATCCTCTTTCTCACAGCAGAGTACATGTGTTGTAACAA ACTTGTCAAAACCTAGGCCTATGAGAATTGCTAAACAGCAGTCATTGGAAACATGTGAGAAAACAGTTTC TGAAAGTTCACAAATGACAGAACATAGAAAGGTTTCTGATCACATACAGTGGTTTAACAAGCTTTCTTTA AATGAACCAAATAGAATAAAAGTCAAGTCACCTCTTAAGTTTCAGCGTACTCCTGTTCGTCAGTCCGTCA GAAGAATTAATTCTTTGTTGGAGTATAGCAGACAACCTACAGGGCATAAGTTGGCGAGTCTTGGTGATAC AGCTTCTCCTTTGGTCAAATCAGTGAGCTGTGACGGTGCTCTTTCCTCTTGTATAGAAAGTGCATCAAAA GATTCCTCTGTTTCATGTATCAAATCAGGTCCTAAAGAACAGAAGTCCATGTCATGTGAAGAGTCAAATA TTGGTGCAATTTCAAAGTCAAGCATGGAGTTACCCTCGAAATCTTTCTTAAAGATGAGGAAGCACCCAGA TTCAGTGAATGCTTCTCTTAGGTCTACTACAGTTTATAAACAGAAGATCTTATCTGATGGCCAAGTTAAG GTTCCCTTGGATGATCTGACTAATCATGATATAGTAAAACCAGTTGTAAATAACAACATGGGCATTTCTT CTGGGATAAATAACAGGGTCCTTAGGAGACCATCAGAAAGAGGAAGGGCCTGGTACAAAGGTTCTCCAAA ACATCCTATCGGAAAAACTCAATTACTACCAACAAGTAAACCTGTAGATTTGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286480
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286480.1, NP_001273409.1
RefSeq Size	5279 bp
RefSeq ORF	2505 bp
Locus ID	9824
UniProt ID	Q6P4F7
Gene Summary	This gene encodes a member of the Rho GTPase activating protein family. In response to DNA damage, the encoded protein interacts with the p53 tumor suppressor protein and stimulates its tetramerization, which results in cell-cycle arrest and apoptosis. A chromosomal deletion that includes this gene is one cause of Prader-Willi syndrome, and an intronic variant of this gene may be associated with sleep duration in children. This gene is highly expressed in colon cancers and in a human basal-like breast cancer cell line. This gene also produces a ARHGAP11A-SCG5 readthrough transcript and ARHGAP11A-SCG5 protein. [provided by RefSeq, Feb 2019] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus than isoform 1. Variants 3 and 4 encode the same isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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