PHF12 (NM_001290131) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290131, the custom clone sequence may differ by one or more nucleotides ATGTGGGAGAAAATGGAGACCAAGACGATCGTGTACGACTTGGACACATCAGGGGGGCTGATGGAGCAAA TCCAAGCTCTGCTGGCTCCCCCCAAGACGGACGAGGCAGAAAAGCGCAGTCGGAAGCCTGAGAAGGAGCC CCGGAGAAGCGGCAGGGCCACCAACCACGACAGCTGCGATAGCTGCAAGGAAGGTGGAGATCTCCTGTGC TGCGACCACTGCCCGGCTGCCTTCCACCTCCAGTGCTGTAACCCTCCACTGAGTGAAGAAATGTTGCCTC CTGGAGAGTGGATGTGTCACCGGTGCACTGTTCGCCGAAAGAAACGAGAGCAGAAAAAGGAGCTGGGTCA TGTCAATGGACTGGTGGACAAATCTGGCAAACGGACTACATCCCCCAGCAGTGACACTGACTTGTTGGAC AGATCGGCCAGCAAAACTGAACTAAAGGCCATTGCCCATGCCCGGATCCTGGAAAGGAGAGCCAGCAGGC CTGGCACACCCACATCCAGCGCCAGCACAGAGACTCCCACCTCTGAGCAGAATGATGTCGACGAAGACAT CATTGACGTGGATGAGGAACCAGTAGCAGCGGAGCCAGACTATGTGCAGCCCCAGCTGAGGCGGCCCTTT GAGCTGCTGATTGCTGCCGCCATGGAGCGGAACCCCACCCAATTTCAGTTGCCCAATGAACTGACTTGTA CCACTGCACTACCAGGTTCTAGCAAGAGGAGAAGAAAGGAGGAAACCACAGGGAAAAATGTTAAGAAGAC ACAGCATGAATTAGATCACAATGGTCTCGTTCCCTTACCCGTCAAAGTCTGCTTCACGTGTAACAGGAGT TGCCGTGTGGCTCCTCTCATCCAGTGTGACTATTGCCCTCTCCTGTTTCACATGGATTGCCTCGAGCCGC CGCTCACTGCCATGCCCCTGGGCAGATGGATGTGTCCGAATCACATCGAACATGTGGTGCTGAACCAGAA GAATATGACACTGAGCAATCGGTGCCAGGTGTTTGATCGTTTCCAGGACACCGTTTCGCAGCATGTCGTC AAAGTGGACTTCCTGAACCGAATCCACAAGAAGCACCCCCCTAACCGGCGTGTGCTCCAGTCGGTCAAAA GAAGAAGCTTGAAGGTTCCTGATGCTATAAAATCTCAGTACCAGTTTCCACCCCCTCTCATTGCACCCGC GGCCATTCGGGACGGGGAGCTGATCTGCAATGGGATCCCTGAGGAATCACAGATGCACCTTTTGAACTCT GAGCACTTAGCCACCCAAGCAGAGCAGCAAGAGTGGCTCTGTAGTGTTGTTGCGCTCCAGTGCAGCATAT TGAAACATTTATCTGCTAAGCAGATGCCTTCGCATTGGGACTCTGAACAGACAGAGAAGGCTGATATTAA GCCTGTTATTGTGACTGACAGCTCAGTCACCACCTCCCTGCAAACAGCTGACAAGACACCTACACCTTCC CACTACCCCTTGTCCTGCCCCTCAGGGATTAGCACCCAGAATTCCCTGAGCTGCTCTCCACCCCACCAGT CCCCAGCCCTAGAGGACATCGGCTGCAGTTCTTGTGCGGAAAAATCCAAGAAAACCCCTTGTGGGACTGC CAATGGGCCAGTGAACACAGAGGTGAAAGCTAATGGCCCACACCTCTACAGCAGCCCTACTGATTCCACG GACCCCCGGCGACTTCCAGGCGCTAACACCCCACTACCAGGCCTCTCACACCGGCAAGGCTGGCCCCGGC CCCTCACGCCACCAGCGGCTGGGGGCCTTCAGAACCACACCGTCGGCATCATTGTGAAGACAGAGAATGC CACTGGCCCCAGCTCTTGCCCCCAGAGGAGTTTGGTTCCTGTCCCAAGCCTGCCCCCTTCCATTCCCAGC TCTTGTGCCAGCATCGAGAACACCAGCACTTTGCAAAGAAAGACTGTCCAATCACAGATAGGACCTCCGT TGACAGATTCAAGGCCACTGGGCTCACCCCCAAACGCCACCCGGGTGCTCACTCCCCCGCAAGCAGCAGG AGATGGTATCTTGGCCACAACAGCCAACCAACGATTCAGCTCACCAGCGCCATCGTCAGATGGCAAGGTC AGCCCCGGCACGTTATCCATAGGAAGCGCTTTAACCGTACCCTCTTTCCCAGCCAACTCTACTGCCATGG TGGACCTCACCAACTCACTTCGAGCATTTATGGATGTCAATGGAGAAATCGAGATAAATATGCTGGACGA GAAGCTGATCAAGTTTCTGGCCTTGCAGAGAATACATCAGCTTTTCCCCTCCCGGGTCCAACCTTCACCG GGCAGTGTCGGGACACATCAGCTGGCTTCTGGAGGGCACCACATAGAAGGTGCGCATGTACACACCTGTC ACCACATCATCAGATCTCCCCTCTACGTGTGCGAATGGTCACCTTGCCTCCATGGAGAATCTGAGCTTCC AGTATCTCAAACCTCCTTGTGTGCTTTTCATGGGCTTAATAAAGCACCATGGTTTTTTTCTGAAGAGAGC AGGAACAAGATGGCAACCACAGCTTTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001290131
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290131.1, NP_001277060.1
RefSeq Size	3912 bp
RefSeq ORF	2550 bp
Locus ID	57649
UniProt ID	Q96QT6
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	Acts as a transcriptional repressor. Involved in recruitment of functional SIN3A complexes to DNA. Represses transcription at least in part through the activity of an associated histone deacetylase (HDAC). May also repress transcription in a SIN3A-independent manner through recruitment of functional TLE5 complexes to DNA.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.