VPS35L (NM_001300743) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C16orf62; EC97
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300743, the custom clone sequence may differ by one or more nucleotides ATGGCGCCGGCGGGAGTTGTAACCCGGGCTGTCCGGAGCGGGGAGCTGCCCCTCACAAGCATGGCGTCAG CTGAAAATGAAGCCTGTGCTGTGCGGAGCGTCGCCTGCCCCTCACAAGCATGGCGTCTGCAGAAGGTGCT GTGCGGCCGCTGCGGGGCGGCCAGCTGCCCTTCACAAACATGGCGGCCGAGGGGTGCGGGGAGTGGCGGG GTAAGGATGGGAAGCCGAGCAGACGGCCCCAGAACAAGCGGTCATGTGACTGGGAAGATGGCCGTCTTTC CTTGGCACTCCAGGAATAGGAACTACAAAGCTGAATTTGCATCATGCCGACTGGAGGCTGTACCATTGGA GTTTGGGGACTATCACCCTCTGAAACCCATAACTGTCACAGAGTCAAAGACAAAGAAAGTGAACCGGAAA GGAAGCACTTCTTCCACGTCCTCCTCCTCCTCCAGCTCCGTGGTGGACCCGCTGAGCAGCGTCCTCGATG GGACTGACCCCCTCTCCATGTTTGCAGCCACTGCTGACCCCGCAGCCTTGGCAGCTGCCATGGACAGCTC CAGAAGGAAACGTGATAGAGATGATAACTCCGTTGTAGGATCGGATTTTGAGCCTTGGACCAACAAACGG GGAGAAATCCTTGCCCGGTACACCACTACCGAAAAGCTGTCTATTAATCTGTTTATGGGATCTGAAAAAG GCAAAGCTGGGACTGCCACATTGGCAATGTCAGAGAAGGTGCGGACCCGGCTGGAGGAGCTGGATGACTT TGAGGAGGGTTCCCAAAAGGAGCTGTTGAACTTGACTCAGCAGGATTACGTGAACCGCATAGAGGAGCTC AACCAATCGCTGAAGGATGCCTGGGCCTCAGACCAGAAAGTGAAGGCTCTAAAAATAGTCATCCAGTGTT CAAAGCTTCTTTCAGACACCAGTGTTATTCAGTTCTACCCAAGCAAATTTGTCCTTATCACCGACATACT TGATACATTTGGAAAGCTCGTGTACGAGCGCATCTTTTCCATGTGTGTGGATAGCCGCAGCGTCTTACCA GATCACTTTTCTCCAGAGAATGCAAATGACACGGCCAAGGAAACATGCCTAAATTGGTTTTTCAAGATTG CCTCCATCAGGGAACTCATTCCAAGATTTTACGTGGAGGCATCCATCCTGAAATGTAACAAATTCCTCTC CAAAACGGGAATTTCAGAGTGCCTGCCCCGGTTGACATGCATGATCAGAGGGATCGGAGACCCACTAGTG TCGGTGTATGCCCGTGCCTACCTGTGCCGGGCTCTGCTGACCGAGATGATGGAAAGGTGTAAGAAACTAG GAAACAATGCCTTGCTGTTGAATTCTGTGATGTCTGCCTTCCGGGCTGAGTTCATCGCCACAAGGTCTAT GGATTTCATTGGCATGATTAAAGAGTGTGATGAATCTGGTTTCCCCAAGCATCTTCTTTTTCGATCACTG GGATTAAACTTGGCCTTGGCTGATCCTCCTGAGAGTGACCGACTTCAGATTCTCAACGAAGCTTGGAAAG TCATCACTAAGCTGAAGAACCCACAGGACTACATTAATTGTGCCGAAGTGTGGGTGGAATACACCTGCAA GCATTTCACGAAACGAGAGGTGAATACCGTTTTGGCAGATGTCATCAAGCACATGACTCCAGATCGTGCA TTTGAAGATTCCTACCCCCAGCTTCAGTTAATAATTAAGAAAGTTATTGCCCACTTCCATGACTTCTCAG TTCTTTTCTCAGTGGAAAAATTTCTGCCGTTTCTGGACATGTTCCAAAAAGAGAGTGTGCGGGTGGAGGT TTGCAAATGCATCATGGACGCCTTTATCAAGCATCAACAAGAGCCCACCAAGGACCCGGTCATCTTGAAT GCCCTTTTGCATGTTTGCAAGACCATGCATGACTCTGTGAATGCACTCACTCTTGAGGATGAGAAAAGAA TGCTGTCATATTTGATTAATGGATTTATAAAAATGGTTTCCTTTGGCCGTGATTTTGAACAACAGCTGAG TTTTTATGTTGAGTCCAGGTCGATGTTTTGCAATCTGGAGCCTGTTCTTGTGCAGTTGATTCATGCCTGT GTTGCCTACTGCTTCATCACCATCCCCTCCCTGGCGGGCATCTTCACACGTCTCAATCTCTACCTGCATT CTGGTCAGGTGGCCTTGGCCAACCAGTGCCTCTCCCAAGCTGATGCTTTTTTCAAAGCCGCTATAAGCCT TGTTCCGGAAGTTCCAAAGATGATTAATATTGATGGGAAGATGCGGCCATCGGAATCGTTCCTTCTGGAA TTCCTCTGCAATTTCTTTTCTACTTTATTAATAGTTCCGGATCATCCTGAACATGGGGTCCTGTTTCTTG TTCGAGAGCTTCTCAACGTGATCCAGGACTACACCTGGGAGGACAACAGCGATGAGAAAATCCGCATCTA CACCTGCGTCCTGCATCTCCTCTCCGCCATGAGCCAGGAGACGTACCTTTACCACATAGACAAAGTGGAC TCCAACGACAGCCTCTACGGGGGAGACTCCAAGTTCCTGGCAGAAAACAACAAGCTGTGTGAGACGGTGA TGGCTCAGATCCTAGAGCATCTGAAAACCCTGGCCAAGGACGAGGCCCTGAAGCGCCAGAGCTCGTTGGG CCTTTCCTTCTTTAACAGCATCTTGGCCCATGGGGACCTACGCAACAACAAGCTCAACCAGCTCTCCGTC AACCTGTGGCACCTGGCACAGAGGCACGGCTGTGCAGACACCAGGACCATGGTGAAAACGCTAGAATACA TCAAGAAGCAAAGCAAACAACCAGACATGACTCATCTGACGGAGCTGGCCCTCAGACTCCCTCTGCAAAC AAGGACCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001300743
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300743.1, NP_001287672.1
RefSeq Size	3615 bp
RefSeq ORF	2880 bp
Locus ID	57020
Gene Summary	Acts as component of the retriever complex. The retriever complex is a heterotrimeric complex related to retromer cargo-selective complex (CSC) and essential for retromer-independent retrieval and recycling of numerous cargos such as integrin alpha-5/beta-1 (ITGA5:ITGB1) (PubMed:28892079). The recruitment of the retriever complex to the endosomal membrane involves CCC and WASH complexes (PubMed:28892079). In the endosomes, drives the retrieval and recycling of NxxY-motif-containing cargo proteins by coupling to SNX17, a cargo essential for the homeostatic maintenance of numerous cell surface proteins associated with processes that include cell migration, cell adhesion, nutrient supply and cell signaling (PubMed:28892079). Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association with the CCC complex and cooperation with the WASH complex on early endosomes. Seems not to be required for CCC complex stability (PubMed:25355947).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks alternate in-frame exons in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1.

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