NEMF (NM_001301732) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	IDDSAPN; NY-CO-1; SDCCAG1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001301732, the custom clone sequence may differ by one or more nucleotides ATGAAGAGCCGCTTTAGCACCATTGACCTCCGCGCCGTACTCGCGGAGCTGAATGCTAGCTTGCTAGGAA TGAGAGTAAACAATGTTTATGATGTGGATAATAAGACATACCTTATTCGTCTTCAAAAACCGGACTTTAA AGCTACACTTTTACTTGAATCTGGCATACGAATTCATACAACAGAATTTGAGTGGCCTAAGAATATGATG CCGTCTAGTTTTGCCATGAAGTGCCGAAAACATTTGAAGAGTCGGAGATTAGTCAGTGCAAAACAGCTTG GTGTGGATAGAATTGTAGATTTTCAATTTGGAAGTGATGAAGCTGCTTACCATTTAATCATTGAGCTCTA TGATAGGGGGAACATTGTTCTTACAGATTATGAGTACGTAATTTTAAATATTCTAAGGTTTCGAACTGAT GAGGCAGATGATGTTAAATTTGCTGTTCGTGAACGCTATCCACTTGATCATGCTAGAGCTGCTGAACCTT TGCTTACTTTGGAAAGGTTGACTGAAATAGTAGCCAGCGCACCTAAGGGTGAACTACTGAAGAGGGTGCT TAACCCATTACTTCCCTATGGACCAGCTCTCATTGAACACTGTCTTTTAGAAAATGGATTCTCGGGTAAT GTCAAAGTGGATGAAAAACTTGAAACTAAAGATATTGAAAAAGTACTTGTTTCTCTGCAGAAAGCAGAAG ACTATATGAAAACAACATCCAACTTCAGTGGGAAGGGATATATCATTCAGAAAAGAGAAATAAAACCATG CTTGGAAGCAGATAAACCAGTTGAAGACATACTGACGTATGAGGAATTTCATCCTTTCTTGTTTTCTCAA CATTCACAATGTCCATATATAGAATTTGAATCATTTGACAAGGCGGTGGATGAATTTTATTCCAAGATAG AAGGCCAGAAAATTGACTTAAAAGCTTTACAACAGGAAAAGCAAGCATTGAAGAAATTAGATAATGTTCG AAAGGATCACGAAAACAGATTGGAAGCTCTTCAGCAGGCTCAGGAAATAGACAAACTGAAAGGAGAGCTC ATAGAAATGAACCTACAAATAGTTGACAGAGCCATTCAGGTAGTTCGAAGTGCTTTAGCTAACCAGATAG ATTGGACAGAAATTGGGTTAATTGTGAAAGAAGCCCAGGCTCAAGGAGACCCTGTTGCAAGTGCAATCAA AGAATTAAAACTACAAACAAACCATGTTACAATGCTGCTAAGAAATCCATACTTGTTATCAGAGGAGGAA GATGATGATGTTGATGGTGACGTCAATGTTGAGAAAAATGAAACTGAACCACCAAAAGGAAAAAAGAAAA AACAAAAGAATAAACAGCTGCAGAAGCCTCAGAAAAATAAGCCCTTACTTGTAGATGTTGATCTCAGCTT GTCAGCATATGCCAATGCCAAAAAGTATTATGATCACAAGAGATATGCTGCTAAGAAAACACAAAAGACT GTTGAAGCTGCTGAGAAGGCATTCAAGTCAGCAGAAAAGAAAACAAAGCAAACATTAAAAGAAGTTCAGA CTGTTACCTCTATTCAAAAAGCAAGAAAAGTATATTGGTTTGAGAAATTTCTGTGGTTCATTAGCTCAGA GAACTATCTAATTATAGGTGGACGAGATCAGCAACAGAATGAAATAATTGTGAAAAGATACTTGACACCA GGAGAACCCATCCCCCCACGGACCTTGACTGAAGCTGGCACAATGGCACTTTGCTACAGTGCTGCTTGGG ATGCACGAGTTATCACTAGTGCTTGGTGGGTGTACCATCATCAGGTATCTAAAACAGCACCAACTGGAGA ATATTTGACAACAGGAAGCTTCATGATAAGAGGAAAAAAGAATTTTCTTCCTCCCTCATATCTAATGATG GGGTTTAGCTTCCTTTTTAAGGTAGATGAGTCTTGTGTTTGGAGACATCAGGGTGAACGAAAAGTCAGAG TACAGGATGAAGACATGGAGACACTGGCAAGTTGTACAAGTGAACTCATATCAGAAGAAATGGAACAATT AGATGGAGGTGACACGAGCAGTGATGAGGATAAAGAAGAACATGAAACTCCTGTGGAAGTAGAACTCATG ACTCAGGTTGACCAAGAGGATATCACTCTTCAGAGTGGCAGAGATGAACTAAATGAGGAGCTCATTCAGG AAGAAAGCTCTGAAGACGAAGGAGAATATGAAGAGGTTAGAAAAGATCAGGATTCTGTTGGTGAAATGAA GGATGAAGGGGAAGAGACATTAAATTATCCTGATACTACCATTGACTTGTCTCACCTTCAACCCCAAAGG TCCATCCAGAAATTGGCTTCAAAAGAGGAATCTTCTAATTCTAGTGACAGTAAATCACAGAGCCGGAGAC ATTTGTCAGCCAAGGAAAGAAGGGAAATGAAAAAGAAAAAACTTCCAAGTGACTCAGGAGATTTAGAAGC GTTAGAGGGAAAGGATAAAGAAAAAGAAAGTACTGTACACATTGAAACTCATCAGAACACAAGCAAAAAT GTTGCGGCTGTGCAGCCAATGAAACGAGGACAAAAGAGTAAAATGAAAAAAATGAAAGAAAAATACAAAG ACCAGGATGAAGAAGACCGTGAACTTATCATGAAGTTGCTGGGGTCTGCAGGTTCAAACAAAGAAGAAAA AGGGAAGAAGGGGAAGAAAGGAAAAACAAAGGACGAACCTGTGAAGAAACAGCCCCAGAAACCTAGAGGT GGACAGAGGGTCTCTGACAACATTAAGAAAGAAACTCCGTTCCTTGAGGTTATAACTCATGAGTTACAAG ACTTTGCTGTAGATGATCCACATGATGACAAGGAAGAGCAAGATCTGGATCAACAGGGAAATGAGGAAAA CCTATTTGATTCTTTGACAGGCCAGCCACATCCTGAAGATGTACTACTGTTTGCCATTCCAATATGTGCC CCTTACACCACCATGACAAACTACAAATATAAAGTGAAACTTACTCCTGGAGTGCAGAAAAAGGGAAAAG CTGCAAAAACAGCCTTGAATAGTTTCATGCATTCCAAAGAAGCAACAGCAAGAGAAAAAGACTTATTCCG CAGCGTAAAGGACACAGATTTATCAAGAAACATTCCTGGCAAAGTGAAAGTGTCTGCACCCAATCTTCTG AACGTAAAAAGGAAATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001301732
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301732.2, NP_001288661.1
RefSeq Size	4740 bp
RefSeq ORF	3168 bp
Locus ID	9147
UniProt ID	O60524
Gene Summary	This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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