WASH complex subunit 7 (WASHC4) (NM_001293640) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KIAA1033; MRT43; SWIP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001293640, the custom clone sequence may differ by one or more nucleotides ATGGCGGTGGAGACTCTGTCCCCGGACTGGGAGTTTGACCGCGTTGACGACGGCTCGCAGAAAATTCATG CCGAAGTCCAACTTAAGAATTATGGGAAATTTCTTGAGGAGTATACCTCTCAACTGAGAAGAATTGAGGA CGCTCTGGATGACTCAATTGGAGATGTTTGGGATTTCAATCTTGATCCTATAGCATTAAAGCTTTTGCCT TATGAACAGTCCTCTCTTTTGGAACTCATAAAGACTGAAAACAAGGTCTTAAACAAAGTCATCACTGTTT ATGCTGCACTTTGTTGTGAAATCAAGAAATTAAAATATGAGGCTGAAACTAAATTTTACAATGGTCTCTT GTTTTATGGAGAAGGAGCTACAGATGCCAGCATGGTGGAAGGTGATTGCCAAATTCAAATGGGGAGATTT ATTTCATTCTTACAGGAACTGTCTTGCTTTGTTACGAGGTGCTATGAAGTGGTGATGAACGTAGTCCACC AGTTGGCTGCCCTCTATATCAGTAACAAGATTGCACCCAAAATTATAGAGACAACTGGAGTTCATTTTCA GACTATGTATGAGCACTTGGGAGAACTGCTAACAGTTTTGCTCACCCTGGATGAAATTATTGATAATCAT ATCACACTGAAAGACCACTGGACTATGTACAAAAGGTTACTGAAATCTGTCCATCACAATCCTTCAAAAT TTGGAATTCAGGAAGAAAAATTAAAGCCATTTGAAAAGTTCTTGCTGAAGCTAGAAGGGCAATTACTGGA TGGAATGATATTCCAGGCCTGTATAGAACAACAATTTGATTCTCTCAATGGAGGAGTATCTGTGTCAAAA AATAGTACTTTTGCTGAGGAATTTGCACATAGTATTCGGTCAATTTTTGCAAATGTAGAAGCCAAACTTG GAGAACCTTCTGAAATTGACCAGAGAGACAAGTATGTTGGAATTTGTGGACTCTTTGTATTGCACTTTCA GATTTTTCGAACTATTGATAAAAAGTTTTATAAGTCTTTATTGGACATTTGTAAGAAGGTACCAGCCATC ACTCTAACTGCTAATATTATTTGGTTTCCTGATAATTTTCTGATCCAGAAAATACCAGCAGCTGCCAAAC TGCTAGACAGAAAAAGTCTTCAAGCCATTAAAATACACAGGGATACTTTTCTACAACAGAAAGCTCAATC ACTTACCAAAGATGTACAGTCTTACTACGTCTTTGTGAGCTCATGGATGATGAAAATGGAATCTATTTTG TCTAAAGAGCAGAGAATGGATAAATTTGCTGAAGATCTCACCAATAGATGTAATGTTTTTATACAGGTAG GCTTCTTGTATGCATATAGTATTAGTACCATTATTAAAACCACAATGAATCTCTACATGTCCATGCAAAA GCCAATGACCAAAACCTCAGTTAAGGCATTGTGCAGGCTTGTTGAACTTCTCAAGGCAATAGAGCATATG TTCTACAGGAGAAGCATGGTTGTGGCTGATTCAGTTTCACATATAACACAGCACCTTCAACATCAGGCTC TTCATTCTATTTCTGTGGCCAAGAAAAGAGTGATTTCTGACAAAAAATACAGCGAACAGCGTCTTGATGT GCTCTCTGCTCTAGTTTTGGCTGAAAACACTCTAAATGGACCAAGCACAAAGCAACGGCGACTTATTGTT TCTTTGGCACTAAGTGTTGGCACACAAATGAAAACATTTAAAGATGAAGAACTCTTTCCACTTCAAGTAG TCATGAAAAAACTGGATCTTATTAGTGAACTTAGAGAACGAGTCCAAACACAATGTGACTGTTGTTTTTT ATACTGGCATCGAGCTGTCTTCCCAATTTATTTAGATGATGTATATGAAAATGCTGTTGATGCAGCCAGA TTACATTACATGTTCAGTGCTTTGCGCGACTGTGTACCTGCTATGATGCATGCAAGGCATTTAGAGTCCT ATGAGATACTTCTGGATTGCTATGACAAGGAAATTATGGAAATTTTAAATGAGCATTTGCTGGACAAATT ATGCAAAGAAATAGAGAAAGATCTGCGACTTTCTGTGCATACTCATTTAAAGCTGGATGACCGAAACCCT TTCAAAGTTGGCATGAAAGACCTGGCTCTTTTTTTCTCTCTGAATCCAATTCGGTTTTTCAATCGTTTCA TTGACATTCGGGCTTACGTAACTCACTACCTAGACAAGACTTTCTACAATCTAACAACTGTAGCCCTTCA TGACTGGGCCACTTATAGTGAGATGAGAAACTTAGCTACTCAGCGTTATGGACTGGTTATGACAGAGGCA CATCTTCCCAGTCAGACTTTGGAACAGGGCCTTGATGTTTTAGAAATTATGAGAAACATTCATATATTTG TGTCCCGATACCTCTATAATCTCAACAATCAGATTTTTATTGAACGAACAAGCAATAACAAGCATTTGAA TACTATTAATATTCGGCATATTGCTAATTCAATTCGAACACATGGCACGGGAATTATGAATACAACTGTT AATTTCACCTACCAGTTTTTGAAAAAGAAGTTCTATATATTTAGCCAATTTATGTATGATGAACACATCA AATCCAGATTGATTAAAGATATTCGATTTTTCAGGGAAATTAAGGACCAAAATGATCATAAGTATCCTTT TGATAGAGCAGAAAAATTCAATCGAGGCATCAGAAAACTTGGAGTAACACCTGAGGGACAGAGCTACCTT GATCAATTCAGGCAACTCATCAGCCAGATTGGTAATGCTATGGGCTATGTACGAATGATAAGATCTGGTG GTCTTCATTGTAGCAGCAATGCCATTAGATTTGTTCCTGATCTTGAAGATATTGTAAATTTTGAAGAACT AGTAAAAGAAGAAGGTCTTGCAGAAGAAACATTAAAAGCAGCAAGGCATTTGGATTCAGTCCTCAGTGAT CACACACGAAATTCTGCCGAAGGCACAGAATATTTCAAAATGCTTGTAGACGTTTTTGCTCCAGAATTTC GAAGGCCAAAGAATATACATCTCCGAAATTTCTATATAATTGTTCCCCCTCTGACCCTCAACTTTGTAGA GCATTCCATTAGTTGCAAGGAAAAATTAAATAAAAAAAATAAAATTGGAGCTGCCTTTACTGATGATGGC TTTGCCATGGGTGTGGCTTACATTCTAAAGCTTTTGGATCAGTATCGGGAGTTTGATTCACTTCACTGGT TCCAGTCTGTTAGAGAGAAATACCTGAAGGAGATAAGAGCAGTTGCTAAGCAACAGAATGTACAGTCAGC CAGTCAAGATGAAAAACTCTTACAAACCATGAATCTCACTCAGAAGCGACTGGATGTCTATCTACAGGAA TTTGAATTGCTGTATTTCTCACTGAGCAGTGCAAGAATTTTCTTCAGAGCAGACAAGACTGCGGCTGAAG AAAACCAAGAAAAGAAAGAGAAGGAAGAAGAAACTAAAACAAGCAATGGAGACCTGTCTGACAGCACTGT GTCTGCTGATCCTGTTGTGAAATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001293640
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293640.1, NP_001280569.1
RefSeq Size	5833 bp
RefSeq ORF	3525 bp
Locus ID	23325
UniProt ID	Q2M389
Gene Summary	This gene encodes a component of the WASH complex, which functions in the intracellular transport of endosomes. Mutations in this gene have been detected in individuals with autosomal recessive cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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