FILIP1 (NM_001300866) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FILIP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300866, the custom clone sequence may differ by one or more nucleotides ATGAGATCTCGAAACCAAGGTGGTGAAAGTGCATCTGATGGGCATATCTCCTGTCCCAAGCCCTCCATCA TCGGCAATGCTGGTGAAAAAAGTCTCTCAGAAGATGCAAAAAAGAAGAAGAAATCAAATAGGAAGGAGGA TGATGTCATGGCCTCAGGAACTGTCAAACGACACCTAAAAACATCTGGAGAATGTGAACGAAAAACTAAG AAATCCCTGGAGTTATCCAAAGAAGACCTCATCCAACTACTCAGTATAATGGAAGGGGAGTTGCAGGCCA GAGAAGATGTGATCCACATGCTGAAGACAGAGAAAACCAAGCCTGAGGTTCTGGAGGCTCATTACGGGTC TGCGGAGCCAGAGAAAGTGCTGCGGGTCCTGCACCGAGATGCCATTCTTGCCCAGGAGAAATCCATAGGA GAAGATGTCTATGAGAAACCGATTTCAGAGCTGGACAGACTTGAGGAAAAACAGAAAGAAACCTACCGGC GCATGCTAGAGCAGCTGTTGCTGGCCGAGAAGTGTCATAGGCGCACCGTATACGAGTTAGAGAACGAGAA GCATAAACACACTGACTACATGAACAAGAGCGACGACTTCACCAACCTGCTGGAGCAGGAGCGGGAGAGG TTAAAAAAGCTCCTTGAACAAGAAAAGGCTTATCAAGCCCGCAAAGAAAAGGAAAATGCTAAACGACTCA ATAAACTAAGAGATGAGCTTGTTAAACTCAAATCCTTTGCACTCATGCTGGTGGATGAAAGACAAATGCA CATTGAACAACTTGGCCTGCAAAGCCAGAAAGTACAGGATCTTACTCAGAAGCTGAGGGAAGAAGAAGAG AAGCTCAAAGCCATTACTTCCAAATCCAAAGAAGACAGACAGAAATTGCTCAAGTTAGAAGTGGACTTTG AACACAAGGCTTCGAGGTTTTCTCAAGAGCATGAAGAGATGAACGCTAAACTGGCTAATCAAGAGTCTCA CAATAGGCAACTTAGACTCAAGCTGGTTGGCTTAACCCAAAGAATCGAGGAGCTAGAAGAGACCAACAAA AATCTGCAGAAGGCAGAGGAAGAACTTCAAGAATTAAGAGATAAAATTGCCAAAGGAGAATGTGGAAACT CTAGCCTCATGGCAGAAGTGGAAAATCTTCGAAAGCGTGTGCTTGAAATGGAAGGTAAAGATGAGGAGAT CACTAAAACTGAATCCCAGTGTAGGGAATTGAGGAAGAAGCTGCAAGAGGAAGAACACCATAGTAAGGAG CTCAGACTTGAAGTTGAGAAGCTACAGAAGAGAATGTCTGAACTAGAGAAATTGGAAGAAGCATTTAGCA AGAGTAAATCTGAGTGCACCCAGCTACATTTAAATCTGGAGAAAGAAAAGAACTTAACCAAAGACCTGCT AAATGAATTGGAGGTGGTCAAGAGTCGAGTTAAAGAATTGGAATGTTCTGAAAGTAGATTGGAAAAGGCT GAATTAAGCCTAAAAGATGATCTTACCAAGTTGAAGTCATTTACCGTGATGCTGGTTGATGAAAGGAAAA ATATGATGGAAAAAATAAAACAAGAAGAGAGAAAAGTGGATGGACTCAATAAAAATTTTAAGGTGGAACA AGGAAAAGTTATGGATGTAACTGAAAAACTAATTGAAGAAAGTAAGAAACTTTTAAAACTAAAATCTGAA ATGGAGGAAAAAGTATACAACTTGACAAGAGAAAGAGATGAGTTGATAGGCAAATTGAAAAGTGAAGAAG AAAAATCCTCTGAATTAAGCTGCAGTGTTGACTTACTAAAGAAGAGACTTGATGGTATAGAGGAAGTGGA AAGAGAAATAACAAGAGGAAGGTCACGAAAAGGGTCTGAGCTCACCTGCCCGGAAGATAATAAGATTAAG GAACTAACACTTGAAATTGAGAGACTGAAGAAACGTCTCCAACAATTGGAAGTGGTCGAAGGGGATTTGA TGAAGACAGAAGATGAGTATGATCAGCTGGAACAGAAATTTAGAACTGAGCAGGATAAGGCTAACTTCCT CTCTCAACAACTAGAGGAGATCAAGCACCAAATTGCCAAGAATAAAGCAATAGAGAAGGGTGAGGTTGTG AGCCAGGAAGCTGAACTGAGACACAGATTTCGGTTGGAAGAAGCTAAAAGTCGAGACTTAAAAGCCGAAG TACAAGCTCTTAAAGAGAAGATTCACGAATTAATGAACAAAGAAGATCAGCTTTCTCAGCTCCAGGTAGA TTATTCTGTACTTCAACAAAGATTTATGGAAGAAGAAAATAAGAACAAAAACATGGGGCAGGAGGTTCTC AATCTGACCAAAGAGTTGGAGCTTTCCAAGCGCTACAGCAGAGCTCTTAGGCCCAGTGTGAATGGAAGAA GAATGGTGGATGTTCCTGTGACGTCAACTGGAGTCCAAACTGATGCAGTCAGCGGTGAAGCAGCAGAGGA AGAAACGCCAGCTGTATTCATACGGAAATCCTTCCAGGAAGAAAATCATATTATGAGTAATCTTCGGCAG GTGGGATTGAAGAAACCCGTGGAAAGATCTTCTGTTCTAGACAGGTATCCTCCAGCAGCAAATGAGCTCA CTATGAGAAAGTCTTGGATTCCATGGATGAGAAAGAGGGAAAACGGCCCCTCCATCACTCAGGAGAAAGG GCCCCGAACAAATTCCAGTCCAGGGCACCCAGGAGAGGTAGTCCTTTCACCAAAGCAGGGCCAGCCCCTG CATATTCGAGTGACACCAGACCACGAGAACAGCACTGCGACTTTGGAGATAACAAGCCCGACATCTGAAG AATTTTTTTCTAGTACCACTGTCATTCCTACCTTAGGGAATCAGAAACCAAGAATAACCATTATTCCATC ACCAAACGTTATGCCTCAAAAACAAAAAAGTGGAGATACTACTCTTGGCCCAGAACGAGCCATGTCCCCA GTCACAATTACTACATTTTCCAGAGAGAAGACTCCAGAAAGTGGAAGAGGCGCATTTGCAGACAGGCCCA CATCCCCTATTCAGATAATGACGGTGTCTACATCAGCAGCACCAGCTGAGATTGCAGTTTCTCCCGAATC CCAGGAAATGCCCATGGGACGGACAATCCTCAAAGTCACCCCAGAAAAACAGACTGTTCCAACTCCAGTA CGGAAATACAACTCCAATGCCAATATCATAACCACAGAGGACAATAAAATTCACATTCACTTAGGGTCTC AGTTTAAACGGTCCCCTGGGACTTCAGGTGAAGGAGTCAGTCCAGTTATTACTGTCCGACCAGTAAACGT GACAGCCGAAAAGGAGGTTTCCACCGGCACTGTCCTTCGCTCTCCCAGGAATCACCTCTCCTCACGGCCT GGTGCAAGCAAAGTGACGAGCACTATCACCATAACACCGGTCACAACGTCATCTGCTCGAGGAACCCAGT CAGTGTCAGGACAAGACGGGTCATCCCAGCGGCCTACACCCACCCGCATTCCTATGTCAAAAGAAAGCAT CATCATTCACCAGTTACGAATGAACTCCAGATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001300866
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300866.1, NP_001287795.1
RefSeq Size	3999 bp
RefSeq ORF	3534 bp
Locus ID	27145
UniProt ID	Q7Z7B0
Gene Summary	This gene encodes a filamin A binding protein. The encoded protein promotes the degradation of filamin A and may regulate cortical neuron migration and dendritic spine morphology. Mice lacking a functional copy of this gene exhibit reduced dendritic spine length and altered excitatory signaling. [provided by RefSeq, Oct 2016] Transcript Variant: This variant (4) has multiple differences compared to variant 1. The encoded protein (isoform 3) has a shorter N-terminus and a shorter, distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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