KDM6A (NM_001291416) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA386N14.2; KABUK2; UTX
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001291416, the custom clone sequence may differ by one or more nucleotides ATGAAATCCTGCGGAGTGTCGCTCGCTACCGCCGCCGCTGCCGCCGCCGCTTTCGGTGATGAGGAAAAGA AAATGGCGGCGGGAAAAGCGAGCGGCGAGAGCGAGGAGGCGTCCCCCAGCCTGACAGCCGAGGAGAGGGA GGCGCTCGGCGGACTGGACAGCCGCCTCTTTGGGTTCGTGAGATTTCATGAAGATGGCGCCAGGACGAAG GCCCTACTGGGCAAGGCTGTTCGCTGCTATGAATCTCTAATCTTAAAAGCTGAAGGAAAAGTGGAGTCTG ATTTCTTTTGTCAATTAGGTCACTTCAACCTCTTATTGGAAGATTATCCAAAAGCATTATCTGCATACCA GAGGTACTACAGTTTACAGTCTGACTACTGGAAGAATGCTGCCTTTTTATATGGTCTTGGTTTGGTCTAC TTCCATTATAATGCATTTCAGTGGGCAATTAAAGCATTTCAGGAGGTGCTTTATGTTGATCCCAGCTTTT GTCGAGCCAAGGAAATTCATTTACGACTTGGGCTTATGTTCAAAGTGAACACAGACTATGAGTCTAGTTT AAAGCATTTTCAGTTAGCTTTGGTTGACTGTAATCCCTGCACTTTGTCCAATGCTGAAATTCAATTTCAC ATTGCCCACTTATATGAAACCCAGAGGAAATATCATTCTGCAAAAGAAGCTTATGAACAACTTTTGCAGA CAGAGAATCTTTCTGCACAAGTAAAAGCAACTGTCTTACAACAGTTAGGTTGGATGCATCACACTGTAGA TCTCCTGGGAGATAAAGCCACCAAGGAAAGCTATGCTATTCAGTATCTCCAAAAGTCCTTGGAAGCAGAT CCTAATTCTGGCCAGTCCTGGTATTTCCTCGGAAGGTGCTATTCAAGTATTGGGAAAGTTCAGGATGCCT TTATATCTTACAGGCAGTCTATTGATAAATCAGAAGCAAGTGCAGATACATGGTGTTCAATAGGTGTGCT ATATCAGCAGCAAAATCAGCCCATGGATGCTTTACAGGCCTATATTTGTGCTGTACAATTGGACCATGGC CATGCTGCAGCCTGGATGGACCTAGGCACTCTCTATGAATCCTGCAACCAGCCTCAGGATGCCATTAAAT GCTACTTAAATGCAACTAGAAGCAAAAGTTGTAGTAATACCTCTGCACTTGCAGCACGAATTAAGTATTT ACAGGCATGTAAACCTCATCATCCAAATACTGAACCTGTATTAGGCCTCAGTCAAACACCAATTTCACAG CAATCCTTGCCACTACACATGATTCCTTCTAGCCAAGTAGATGACCTGTCCAGTCCTGCCAAGAGGAAAA GAACATCTAGTCCAACAAAGAATACTTCTGACAATTGGAGTGGTGGACATGCTGTGTCACATCCTCCAGT ACAGCAACAAGCTCATTCATGGTGTTTGACACCACAGAAATTACAGCATTTGGAACAGCTCCGCGCAAAT AGAAATAATTTAAATCCAGCACAGAAACTGATGCTGGAACAGCTGGAAAGTCAGTTTGTCTTAATGCAAC AACACCAAATGAGACCAACAGGAGTTGCACAGGTACGATCTACTGGAATTCCTAATGGGCCAACAGCTGA CTCATCACTGCCTACAAACTCAGTCTCTGGCCAGCAGCCACAGCTTGCTCTGACCAGAGTGCCTAGCGTC TCTCAGCCTGGAGTCCGTCCTGCCTGCCCTGGGCAGCCTTTGGCCAATGGACCCTTTTCTGCAGGCCATG TTCCCTGTAGCACATCAAGAACGCTGGGAAGTACAGACACTATTTTGATAGGCAATAATCATATAACAGG AAGTGGAAGTAATGGAAACGTGCCTTACCTGCAGCGAAACGCACTCACTCTACCTCATAACCGCACAAAC CTGACCAGCAGCGCAGAGGAGCCGTGGAAAAACCAACTATCTAACTCCACTCAGGGGCTTCACAAAGGTC AGAGTTCACATTCGGCAGGTCCTAATGGTGAACGACCTCTCTCTTCCACTGGGCCTTCCCAGCATCTCCA GGCAGCTGGCTCTGGTATTCAGAATCAGAACGGACATCCCACCCTGCCTAGCAATTCAGTAACACAGGGG GCTGCTCTCAATCACCTCTCCTCTCACACTGCTACCTCAGGTGGACAACAAGGCATTACCTTAACCAAAG AGAGCAAGCCTTCAGGAAACATATTGACGGTGCCTGAAACAAGCAGGCACACTGGAGAGACACCTAACAG CACTGCCAGTGTCGAGGGACTTCCTAATCATGTCCATCAGATGACGGCAGATGCTGTTTGCAGTCCTAGC CATGGAGATTCTAAGTCACCAGGTTTACTAAGTTCAGACAATCCTCAGCTCTCTGCCTTGTTGATGGGAA AAGCCAATAACAATGTGGGTACTGGAACCTGTGACAAAGTCAATAACATCCACCCAGCTGTTCATACAAA GACTGATAACTCTGTTGCCTCTTCACCATCTTCAGCCATTTCAACAGCAACACCTTCTCCAAAATCCACT GAGCAGACAACCACAAACAGTGTTACCAGCCTTAACAGCCCTCACAGTGGGCTACACACAATTAATGGAG AAGGGATGGAAGAATCTCAGAGCCCCATGAAAACAGATCTGCTTCTGGTTAACCACAAACCTAGTCCACA GATCATACCATCAATGTCTGTGTCCATATACCCCAGCTCAGCAGAAGTTCTGAAGGCATGCAGGAATCTA GGTAAAAATGGCTTATCTAACAGTAGCATTTTGTTGGATAAATGTCCACCTCCAAGACCACCATCTTCAC CATACCCTCCCTTGCCAAAGGACAAGTTGAATCCACCTACACCTAGTATTTACTTGGAAAATAAACGTGA TGCTTTCTTTCCTCCATTACATCAATTTTGTACAAATCCGAACAACCCTGTTACAGTAATACGTGGCCTT GCTGGAGCTCTTAAGTTAGACCTGGGACTTTTCTCTACTAAAACTTTGGTGGAAGCTAACAATGAACATA TGGTAGAAGTGAGGACACAGTTGTTGCAGCCAGCAGATGAAAACTGGGATCCCACTGGAACAAAGAAAAT CTGGCATTGTGAAAGTAATAGATCTCATACTACAATTGCTAAATATGCACAGTACCAGGCCTCCTCATTC CAGGAATCATTGAGAGAAGAAAATGAAAAAAGAAGTCATCATAAAGACCACTCAGATAGTGAATCTACAT CGTCAGATAATTCTGGGAGGAGGAGGAAAGGACCCTTTAAAACCATAAAGTTTGGGACCAATATTGACCT ATCTGATGACAAAAAGTGGAAGTTGCAGCTACATGAGCTGACTAAACTTCCTGCTTTTGTGCGTGTCGTA TCAGCAGGAAATCTTCTAAGCCATGTTGGTCATACCATATTGGGCATGAACACAGTTCAACTATACATGA AAGTTCCAGGGAGCAGAACACCAGGTCATCAGGAAAATAACAACTTCTGTTCAGTTAACATAAATATTGG CCCAGGTGACTGTGAATGGTTTGTTGTTCCTGAAGGTTACTGGGGTGTTCTGAATGACTTCTGTGAAAAA AATAATTTGAATTTCCTAATGGGTTCTTGGTGGCCCAATCTTGAAGATCTTTATGAAGCAAATGTTCCAG TGTATAGGTTTATTCAGCGACCTGGAGATTTGGTCTGGATAAATGCAGGCACTGTTCATTGGGTTCAGGC TATTGGCTGGTGCAACAACATTGCTTGGAATGTTGGTCCACTTACAGCCTGCCAGTATAAATTGGCAGTG GAACGGTACGAATGGAACAAATTGCAAAGTGTGAAGTCAATAGTACCCATGGTTCATCTTTCCTGGAATA TGGCACGAAATATCAAGGTCTCAGATCCAAAGCTTTTTGAAATGATTAAGTATTGTCTTCTAAGAACTCT GAAGCAATGTCAGACATTGAGGGAAGCTCTCATTGCTGCAGGAAAAGAGATTATATGGCATGGGCGGACA AAAGAAGAACCAGCTCATTACTGTAGCATTTGTGAAGTGGAGGTTTTTGATCTGCTTTTTGTCACTAATG AGAGTAATTCACGAAAGACCTACATAGTACATTGCCAAGATTGTGCACGAAAAACAAGCGGAAACTTGGA AAACTTTGTGGTGCTAGAACAGTACAAAATGGAGGACCTGATGCAAGTCTATGACCAATTTACATTAGCT CCTCCATTACCATCCGCCTCATCTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001291416
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291416.1, NP_001278345.1
RefSeq Size	5806 bp
RefSeq ORF	4227 bp
Locus ID	7403
Gene Summary	This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.

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