DOCK10 (NM_001290263) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DRIP2; Nbla10300; ZIZ3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290263, the custom clone sequence may differ by one or more nucleotides ATGAGTTTTCGAGGGAAGGTTTTTAAACGGGAGCCCAGTGAATTTTGGAAGAAGAGACGAACTGTGAGGA GAGTAAACCAAGAAGAAATCCACAGATTTAGTTCTCAAGAAAAGCCTAGGCTTCTCGAGCCTTTGGATTA TGAGACTGTCATTGAAGAACTTGAAAAGACCTACCGGAATGATCCTCTTCAAGATCTCTTGTTCTTCCCC AGTGATGACTTTTCAGCAGCCACAGTTTCCTGGGATATCCGCACGTTGTACTCAACAGTACCTGAAGATG CAGAGCACAAGGCAGAAAATTTACTGGTTAAGGAGGCTTGTAAATTTTATAGTTCCCAGTGGCATGTGGT AAACTACAAATATGAACAATATTCTGGAGACATTCGACAGCTACCCCGAGCAGAATACAAACCAGAGAAG CTTCCTTCACATTCCTTTGAGATTGACCATGAAGATGCTGATAAGGATGAAGATACCACTTCCCACTCGT CTTCCAAGGGGGGTGGAGGAGCGGGAGGAACTGGTGTTTTCAAGTCCGGCTGGCTCTACAAGGGGAATTT TAACAGCACCGTGAACAACACCGTTACTGTTCGGTCATTCAAAAAGCGCTACTTCCAGCTGACTCAGTTA CCAGATAACTCCTACATTATGAACTTTTACAAAGATGAGAAAATATCCAAAGAACCCAAAGGATGCATCT TTTTGGATTCCTGTACAGGAGTGGTGCAGAATAACAGACTAAGAAAATATGCCTTTGAATTGAAAATGAA TGATCTGACCTATTTTGTGCTGGCAGCTGAAACAGAGTCAGATATGGATGAATGGATCCACACCCTCAAC CGCATTCTGCAAATCAGTCCTGAGGGGCCCCTCCAAGGGAGGAGGAGCACAGAGCTCACTGATCTGGGTC TGGATTCGCTGGATAATTCTGTAACTTGTGAATGCACGCCAGAGGAAACAGATTCTTCAGAGAACAACCT ACACGCAGACTTTGCAAAGTACCTCACAGAAACAGAAGATACTGTAAAAACAACTCGAAACATGGAGAGG CTAAATCTGTTCTCTCTAGATCCAGACATAGATACCTTGAAACTTCAAAAAAAAGATCTCTTGGAACCTG AGTCTGTGATCAAACCATTTGAAGAAAAAGCTGCCAAGAGAATCATGATCATCTGTAAAGCCCTCAACTC AAATCTTCAGGGATGTGTTACGGAGAATGAAAATGATCCGATAACGAATATTGAGCCTTTTTTTGTGAGT GTGGCACTTTATGACCTCAGAGACAGCAGGAAGATTTCTGCTGATTTTCATGTGGATCTAAACCATGCTG CTGTCAGACAGATGCTCTTGGGGGCTTCTGTGGCTTTGGAAAATGGCAACATCGACACCATCACTCCAAG ACAATCAGAAGAACCTCACATCAAGGGACTTCCAGAGGAATGGCTAAAATTTCCAAAGCAGGCTGTATTT TCTGTAAGCAATCCACATTCTGAAATTGTTTTGGTGGCCAAAATCGAAAAAGTCTTGATGGGAAACATTG CAAGTGGTGCCGAACCTTATATTAAGAACCCAGACTCCAACAAGTATGCACAAAAGATACTAAAATCCAA CAGACAATTCTGCAGCAAATTGGGAAAATACCGTATGCCTTTTGCTTGGGCAGTAAGATCAGTATTTAAG GACAACCAGGGAAATGTGGACAGAGACTCAAGATTTTCACCATTGTTTAGACAAGAAAGTAGCAAGATTT CAACTGAGGACCTAGTTAAACTAGTATCAGATTATAGAAGGGCCGACAGAATAAGCAAAATGCAGACCAT TCCTGGAAGCCTGGATATTGCTGTTGACAACGTTCCCTTGGAGCATCCAAATTGTGTAACATCGTCCTTT ATCCCTGTCAAGCCTTTCAACATGATGGCTCAAACAGAACCCACAGTGGAGGTGGAAGAATTTGTTTACG ATTCAACAAAGTATTGTCGGCCTTACAGAGTATATAAAAATCAAATTTATATTTACCCCAAACACCTCAA GTATGATAGCCAGAAATGCTTCAACAAGGCACGGAATATAACTGTGTGCATTGAATTCAAAAATTCAGAT GAAGAAAGTGCCAAGCCCCTGAAGTGTATTTATGGAAAACCTGGAGGGCCCCTCTTCACCTCAGCCGCCT ACACAGCAGTTCTGCACCACTCTCAGAATCCGGATTTCTCAGATGAGGTGAAAATTGAGCTACCAACACA ACTCCATGAGAAACACCATATTTTGTTTTCTTTTTATCACGTCACCTGTGACATCAATGCAAAAGCTAAT GCCAAAAAGAAGGAGGCTCTGGAAACGTCAGTTGGATATGCTTGGCTTCCTCTGATGAAACACGATCAGA TAGCTTCTCAAGAGTACAACATCCCAATAGCAACAAGTCTGCCTCCTAATTATTTAAGCTTTCAAGATTC TGCAAGTGGAAAGCATGGTGGGAGTGACATTAAATGGGTTGATGGTGGCAAACCACTTTTCAAAGTGTCG ACATTTGTTGTATCAACAGTAAATACTCAGGATCCACATGTGAATGCATTTTTCCAAGAGTGCCAAAAAA GAGAGAAAGATATGTCTCAGTCACCTACCTCAAATTTCATCCGCTCTTGTAAGAACTTATTGAATGTGGA AAAGATTCATGCAATCATGAGTTTTCTGCCTATAATTTTGAATCAGCTCTTCAAAGTTCTGGTACAGAAT GAGGAAGATGAAATAACTACAACTGTCACCAGGGTTCTGACCGACATTGTGGCCAAGTGCCATGAGGAGC AGCTGGATCATTCTGTCCAGTCATATATTAAGTTCGTGTTCAAGACCAGGGCATGCAAGGAGAGGACTGT ACATGAGGAACTGGCTAAAAATGTGACTGGTCTTTTGAAATCAAATGACTCAACAACAGTAAAGCATGTC CTAAAGCATTCCTGGTTCTTCTTTGCAATTATCCTAAAATCGATGGCACAGCACTTGATTGACACAAATA AAATCCAGCTTCCCCGGCCTCAGAGATTTCCTGAATCTTACCAAAATGAATTGGACAATCTTGTCATGGT CCTATCCGACCATGTGATTTGGAAATACAAGGATGCACTTGAAGAAACAAGAAGGGCAAACCACAGCGTT GCCAGATTTCTCAAGCGCTGCTTTACATTTATGGACCGAGGGTATGTGTTTAAGATGGTCAACAATTACA TCAGCATGTTCTCCTCCGGTGACCTTAAGACCTTGTGCCAGTATAAATTTGATTTTCTTCAAGAAGTATG TCAACATGAACACTTTATCCCTTTGTGTCTGCCCATAAGATCAGCAAACATTCCAGATCCTTTGACACCT TCAGAATCGACTCAAGAGTTACATGCATCAGATATGCCTGAATATTCAGTCACAAATGAATTTTGTCGCA AACACTTCTTAATCGGAATTCTGCTCCGAGAAGTTGGCTTTGCCCTGCAGGAAGACCAAGATGTCAGACA CTTAGCTTTAGCTGTCCTAAAAAATCTAATGGCTAAGCATTCATTTGATGATCGATACAGAGAGCCAAGA AAGCAGGCCCAGATAGCAAGTTTATACATGCCCCTGTACGGCATGCTCCTGGACAATATGCCAAGGATTT ATCTGAAGGACCTGTATCCTTTTACTGTCAATACATCTAATCAGGGGTCTAGAGATGATCTAAGCACCAA TGGAGGATTTCAAAGCCAGACAGCTATCAAACATGCAAACTCTGTGGATACATCATTTTCTAAAGATGTT TTAAATTCCATAGCAGCATTTTCATCAATAGCTATTTCTACAGTAAACCATGCTGACTCCAGAGCATCTT TAGCAAGTCTTGACTCCAATCCAAGTACCAATGAGAAGAGCAGTGAGAAGACGGACAACTGTGAAAAGAT CCCAAGACCCTTGTCTTTGATTGGCTCAACTCTTCGATTTGACAAGTTAGATCAAGCAGAAACCAGGAGT CTCCTGATGTGTTTTCTTCACATTATGAAAACGATTTCGTACGAGACTCTGATTGCCTACTGGCAGAGAG CTCCCAGCCCAGAGGTGTCCGACTTCTTCAGCATCTTGGACGTTTGTCTTCAAAATTTCAGATACCTAGG AAAACGCAACATAATAAGAAAAATTGCTGCTGCATTTAAATTTGTGCAGTCCACCCAGAACAATGGAACT CTCAAAGGATCCAATCCTTCCTGCCAGACATCAGGTCTCTTGTCACAATGGATGCACTCCACTTCCAGTC ATGAAGGCCATAAGCAGCACAGATCACAAACTTTACCTATAATTCGAGGCAAAAATGCACTTTCTAACCC CAAACTCTTACAGATGTTAGACAATACCATGACCAGCAACTCCAATGAAATAGACATCGTGCATCATGTA GACACTGAGGCCAATATAGCTACGGAGGTTTGCCTCACTATTCTGGACCTGTTATCCCTCTTCACACAGA CTCATCAGAGACAACTCCAACAATGTGACTGTCAAAATTCATTGATGAAAAGGGTCTTTGATACCTACAT GCTCTTTTTCCAAGTCAATCAGTCAGCCACAGCGCTGAAGCATGTGTTTGCCTCCTTGAGACTGTTTGTA TGCAAGTTTCCTTCAGCGTTCTTTCAAGGGCCTGCTGACCTCTGTGGATCATTCTGTTACGAAGTCCTAA AATGCTGTAACCACAGGTCACGGTCAACTCAGACAGAAGCCTCAGCCCTTCTGTACTTTTTCATGAGGAA GAATTTTGAATTTAACAAGCAGAAGTCAATTGTCCGGTCCCACTTACAACTCATCAAAGCTGTGAGCCAG TTAATAGCCGATGCTGGGATTGGAGGCTCTCGGTTTCAACATTCGCTTGCAATTACCAATAATTTCGCCA ATGGAGATAAGCAAATGAAAAACAGCAATTTCCCAGCAGAGGTGAAGGACCTGACTAAGCGTATAAGGAC TGTTTTGATGGCCACAGCTCAGATGAAGGAGCACGAGAAGGACCCCGAGATGCTGGTGGATCTCCAGTAC AGCCTGGCAAACTCCTACGCAAGCACTCCTGAACTACGCAGGACCTGGCTGGAAAGTATGGCCAAGATTC ATGCCAGAAACGGAGATTTATCTGAGGCTGCCATGTGTTACATCCATATTGCTGCTCTCATTGCAGAGTA TCTGAAAAGAAAGGGTTACTGGAAAGTGGAAAAGATTTGCACAGCATCCCTGCTCTCGGAGGATACCCAC CCCTGTGATAGCAACTCATTACTAACAACTCCCAGTGGAGGAAGCATGTTCTCTATGGGATGGCCAGCTT TTTTGAGCATTACACCAAACATTAAGGAAGAAGGAGCGATGAAAGAGGATTCTGGAATGCAAGATACACC ATACAATGAGAATATCCTGGTGGAGCAGCTATACATGTGTGTGGAGTTTCTCTGGAAGTCTGAGCGATAT GAACTCATTGCTGATGTCAACAAGCCCATCATTGCTGTCTTTGAGAAACAACGAGACTTCAAAAAATTGT CAGATCTCTACTACGACATTCATCGGTCATATCTGAAAGTGGCAGAGGTGGTGAATTCGGAGAAGCGGCT GTTTGGTCGCTACTATCGTGTGGCATTTTATGGGCAGGGCTTTTTTGAAGAAGAAGAAGGTAAAGAGTAT ATTTATAAAGAGCCTAAGCTGACAGGTCTGTCCGAGATTTCCCAAAGATTACTCAAGCTCTATGCAGATA AATTTGGAGCAGACAATGTGAAGATAATCCAGGATTCCAACAAGGTAAACCCCAAGGATTTGGACCCCAA ATATGCCTACATCCAGGTGACCTATGTGACGCCGTTCTTTGAGGAAAAGGAAATCGAAGACCGGAAGACA GATTTCGAAATGCACCACAACATCAACCGCTTTGTCTTCGAGACACCCTTCACGCTGTCGGGCAAGAAGC ACGGTGGGGTGGCGGAGCAGTGCAAGCGGCGGACGATCCTGACAACGAGTCACCTGTTCCCCTACGTGAA GAAGAGAATACAAGTAATTAGCCAATCGAGCACAGAACTGAATCCAATTGAAGTGGCAATTGACGAGATG TCCAAGAAGGTTTCTGAGCTTAATCAGCTTTGCACAATGGAAGAAGTGGACATGATCAGACTGCAGCTCA AACTGCAAGGAAGTGTCAGCGTGAAGGTTAATGCTGGGCCAATGGCCTATGCACGAGCTTTTCTTGAAGA AACCAATGCAAAGAAGTACCCTGACAACCAAGTAAAGCTTTTGAAGGAGATCTTCAGGCAATTTGCAGAT GCATGTGGGCAGGCCCTTGACGTGAATGAGCGCCTCATCAAAGAGGACCAGCTGGAGTACCAGGAAGAAC TGAGGTCCCACTACAAGGACATGCTCAGCGAACTCTCCACAGTCATGAATGAGCAGATTACGGGCAGGGA CGACCTGTCAAAGCGCGGAGTGGACCAAACCTGCACTCGAGTAATTAGCAAAGCAACTCCGGCCCTACCC ACGGTCTCCATCTCATCTAGTGCTGAAGTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001290263
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290263.1, NP_001277192.1
RefSeq Size	7288 bp
RefSeq ORF	6543 bp
Locus ID	55619
UniProt ID	Q96BY6
Gene Summary	This gene encodes a member of the dedicator of cytokinesis protein family. Members of this family are guanosine nucleotide exchange factors for Rho GTPases and defined by the presence of conserved DOCK-homology regions. The encoded protein belongs to the D (or Zizimin) subfamily of DOCK proteins, which also contain an N-terminal pleckstrin homology domain. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (2; also known as DOCK10.2) represents use of an alternate promoter, differs in the 5' UTR and 5' coding region, and initiates translation at a distinct start codon, compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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