OTOG (NM_001292063) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DFNB18B; MLEMP; OTGN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001292063, the custom clone sequence may differ by one or more nucleotides ATGGGAGTCCTGGCGTCTGCGCTCTGCTGGCTGCTTTGTGTCTGGCTGCCCTGGGGTGAGCAGGCAGCCG AGTCCCTGCGGGTGCAGCGCCTCGCCGCAGCACCCGTTCTGTGGGGCAGTGCAGAGCCACAGCCAGAGCC AGCCGGGCAACCCAGCAGCAGCCACCAGGAGGCGACCCTTGCCATGGGGGACAAGGCTACAGTCGTGGGA GGCCAGCAGGCTGAAGCCCCAGACTCCGTGGCCATGTCTTCCTGGGAAAGGCGGCTCCATCGGGCCAAGT GTGCACCATCCTACTTGTTCTCCTGCTTCAATGGAGGCGAGTGTGTGCACCCAGCCTTCTGTGACTGCAG ACGCTTCAATGCCACTGGACCGCGCTGCCAGATGGTGTACAATGCCGGCCCTGAGAGGGACAGCATTTGC CGGGCGTGGGGGCAGCACCACGTGGAGACATTTGATGGGCTCTACTACTACCTCTCCGGAAAGGGCAGCT ACACCCTGGTGGGTCGCCATGAGCCCGAGGGACAGAGCTTCTCCATCCAGGTACACAATGACCCGCAGTG TGGCTCTTCACCCTACACCTGCTCCAGGGCTGTCAGCCTCTTCTTTGTGGGTGAGCAGGAGATCCATCTG GCCAAGGAGGTCACCCATGGAGGCATGAGGGTCCAACTGCCACATGTCATGGGGAGCGCGCGTCTGCAGC AGCTTGCCGGCTATGTCATCGTGCGGCATCAGTCAGCCTTCACACTGGCCTGGGATGGTGCCTCGGCTGT CTACATCAAGATGAGTCCAGAGCTTCTGGGCTGGACCCATGGGCTGTGTGGGAACAACAATGCTGACCCC AAGGATGATCTGGTGACCAGCTCTGGGAAGCTGACTGACGACGTGGTTGAGTTTGTGCACAGCTGGCAGG AGCAGGCCCCTAACCAGCCTCCAGGGCCCACAACTTCCTCCCTGCCTCGCCCACCGTGCCTACAGCAGAA CCCAGGAACCATGCAGGGCGTGTACGAGCAGTGTGAGGCTCTACTGCGGCCCCCCTTTGACGCCTGCCAC GCCTACGTCAGCCCTCTGCCCTTCACAGCCAGTTGTACCAGTGATCTCTGCCAATCAATGGGTGATGTAG CCACCTGGTGCCGGGCACTGGCGGAGTATGCCCGGGCGTGTGCCCAGGCAGGGCGGCCCTTGCAAGGCTG GAGGACCCAGCTCCGGCAATGCACTGTGCACTGCAAGGAGAAGGCCTTTACCTACAATGAGTGCATCGCC TGCTGCCCTGCCTCCTGCCATCCCCGGGCATCCTGTGTGGACAGTGAGATCGCCTGTGTGGACGGCTGCT ATTGCCCCAATGGGCTCATCTTCGAGGATGGGGGCTGCGTGGCACCAGCTGAGTGTCCCTGTGAGTTTCA CGGGACTCTGTACCCACCTGGCTCTGTGGTGAAGGAAGACTGCAATACTTGCACATGCACCTCAGGCAAG TGGGAGTGCAGCACAGCTGTCTGCCCAGCTGAGTGCTCAGTGACTGGTGACATTCACTTCACAACCTTTG ATGGCCGCCGGTACACGTTCCCCGCCACATGTCAGTACATCCTGGCCAAGAGCCGCTCTTCGGGCACCTT CACCGTGACATTGCAGAATGCCCCATGTGGCCTGAACCAAGATGGAGCCTGTGTCCAGTCAGTGTCAGTG ATTCTGCACCAGGACCCTCGGAGGCAGGTGACCCTGACCCAGGCAGGGGATGTCCTTCTGTTTGACCAGT ACAAGATCATCCCGCCATACACAGATGATGCCTTTGAGATCCGTAGGCTGTCCTCCGTGTTCCTGCGGGT GAGGACGAACGTGGGCGTGCGGGTGCTCTACGACCGTGAAGGGCTCCGACTGTACCTGCAAGTGGACCAG CGATGGGTGGAGGATACCGTGGGCCTCTGCGGCACCTTCAATGGCAACACGCAGGATGACTTCCTGTCTC CAGTGGGTGTACCTGAGAGCACCCCACAACTTTTTGGCAATTCCTGGAAAACACTTTCTGCTTGCTCCCC GCTGGTCTCTGGCTCCCCTCTGGACCCCTGCGATGTGCACCTGCAAGCCGCCTCCTACTCAGTGCAGGCC TGCAGCGTGCTCACGGGGGAGATGTTTGCGCCCTGCTCTGCGTTCCTGAGCCCCGTGCCCTACTTTGAGC AGTGCCGCAGGGATGCCTGCCGCTGCGGGCAGCCCTGCCTGTGCGCCACACTGGCCCACTACGCCCACCT GTGCCGGCGCCATGGGCTCCCCGTTGATTTCCGCGCCCGCCTGCCAGCCTGTGCACTGTCCTGTGAGGCC TCCAAGGAGTATAGCCCCTGCGTGGCCCCGTGTGGACGTACCTGCCAGGACCTGGCCAGCCCTGAGGCCT GTGGGGTTGATGGTGGCGATGACCTGAGCAGAGACGAGTGTGTGGAGGGCTGTGCCTGCCCACCGGACAC CTATCTGGACACCCAGGCTGACCTCTGTGTCCCCCGGAACCAGTGCTCCTGCCACTTCCAGGGAGTGGAC TATCCCCCCGGAGACAGTGACATCCCATCCCTGGGCCACTGCCACTGCAAAGATGGAGTCATGAGCTGTG ATAGCAGAGCCCCAGCTGCTGCCTGCCCAGCAGGCCAGGTCTTCGTGAACTGCAGCGACCTGCACACGGA CCTGGAGCTGAGCAGGGAGAGGACGTGTGAGCAGCAACTGCTGAACCTGAGCGTGTCAGCCCGTGGCCCC TGCCTCTCGGGCTGCGCCTGTCCCCAGGGTCTGCTCAGACACGGGGATGCATGTTTCCTGCCAGAGGAGT GCCCCTGCACTTGGAAGGGGAAGGAGTATTTCCCTGGGGACCAGGTGATGTCTCCTTGCCATACCTGTGT GTGCCAGCGGGGCTCATTCCAGTGCACCCTGCACCCTTGCGCCTCCACCTGCACTGCCTATGGGGACCGG CATTACCGCACGTTTGATGGGCTCCCGTTTGACTTCGTGGGGGCATGCAAAGTGCACCTGGTCAAGAGCA CATCAGATGTCAGCTTCTCTGTGATTGTAGAGAATGTGAACTGCTACAGCTCTGGCATGATCTGCAGGAA ATTTATTTCCATCAACGTTGGGAACTCACTCATTGTCTTTGATGATGACTCCGGAAATCCTAGTCCAGAG AGCTTCCTGGATGACAAGCAGGAGGTCCACACATGGCGAGTGGGATTTTTCACACTGGTGCATTTCCCAC AGGAGCACATCACCCTCTTGTGGGACCAGAGAACCACAGTGCACGTCCAGGCTGGGCCTCAGTGGCAGGG CCAGCTGGCGGGCCTCTGTGGGAACTTTGACTTAAAAACCATCAATGAGATGAGGACCCCGGAGAACCTA GAGCTAACTAACCCCCAGGAGTTTGGCAGCAGTTGGGCTGCAGTTGAGTGCCCAGACACCCTCGATCCTC GGGATATGTGTGTCCTGAATCCTCTCCGAGAACCATTTGCCAAGAAGGAGTGCAGCATCCTGCTCAGTGA GGTGTTTGAGATCTGCCACCCTGTGGTTGATGTCACTTGGTTTTACTCAAACTGCCTGACAGACACATGT GGCTGCAGCCAGGGTGGTGACTGTGAGTGCTTCTGTGCCAGCGTCTCCGCTTATGCCCACCAGTGTTGCC AGCATGGGGTGGCTGTTGACTGGCGAACCCCCCGCCTCTGCCCGTATGACTGTGACTTCTTTAACAAAGT GCTAGGTAAGGGCCCCTATCAGCTATCCAGCTTGGCAGCCGGTGGTGCTCTGGTGGGCATGAAGGCGGTG GGCGATGACATAGTCCTAGTGAGGACAGAGGATGTGGCGCCAGCAGACATTGTGAGCTTCCTGCTGACAG CTGCTCTGTACAAGGCCAAGGCCCATGACCCAGATGTGGTGTCCCTGGAGGCAGCAGACAGACCCAACTT CTTCCTTCACGTCACAGCCAACGGGTCTCTGGAGCTGGCTAAGTGGCAGGGCCGTGACACCTTCCAACAG CATGCCTCCTTCTTGCTGCACCGGGGGACACGGCAGGCAGGCCTGGTGGCCCTGGAGTCCCTGGCCAAGC CCAGCTCCTTCCTCTATGTGTCGGGCGCGGTGCTGGCCCTGCGGCTGTACGAACACACAGAGGTGTTCCG CCGGGGCACACTCTTCCGCCTTCTGGATGCCAAGCCCTCGGGGGCTGCCTACCCCATCTGCGAGTGGCGC TACGATGCCTGTGCCAGCCCCTGCTTCCAAACCTGCCGGGACCCACGGGCAGCCAGCTGCCGGGACGTAC CCAGGGTAGAAGGCTGTGTCCCTGTGTGCCCCACCCCCCAGGTCCTGGATGAAGTCACACAGAGATGTGT CTACTTGGAGGACTGTGTGGAGCCAGCAGTTTGGGTTCCCACAGAGGCCCTTGGCAATGAGACCCTCCCT CCCAGTCAAGGGTTGCCCACTCCCAGTGATGAGGAGCCACAGCTGTCACAGGAAAGCCCCAGGACCCCCA CCCACAGGCCAGCCCTCACCCCAGCTGCCCCACTCACCACAGCCCTGAACCCACCAGTGACAGCCACTGA GGAGCCAGTGGTGTCTCCAGGCCCCACCCAGACCACCCTGCAGCAGCCACTGGAGCTCACTGCATCTCAA CTCCCCGCCGGCCCCACGGAGTCCCCAGCCAGCAAGGGAGTGACTGCCAGCCTCCTGGCCATCCCCCATA CACCAGAGTCCTCATCCCTCCCTGTTGCACTGCAGACACCCACACCTGGCATGGTGTCAGGTGCCATGGA GACAACAAGGGTGACTGTGATCTTTGCAGGAAGCCCTAACATCACAGTCTCCTCCCGGTCGCCCCCTGCC CCTCGCTTCCCGCTCATGACCAAGGCTGTGACAGTCCGAGGCCATGGCTCCTTGCCTGTTAGGACGACAC CCCCACAGCCCTCCTTGACAGCAAGTCCCTCCTCCAGACCTGTGGCTTCCCCTGGAGCCATCTCCAGGTC CCCCACCTCCTCGGGATCCCACAAGGCTGTGCTGACACCTGCAGTAACTAAGGTCATAAGCAGGACAGGG GTCCCCCAGCCCACCCAGGCCCAGAGTGCTTCAAGTCCCAGCACCCCTCTAACTGTGGCTGGAACAGCAG CAGAACAGGTTCCTGTCAGTCCCCTTGCAACCAGGAGCTTGGAGATAGTGCTATCCACAGAGAAGGGCGA AGCCGGGCACAGCCAGCCCATGGGCTCGCCTGCCTCCCCACAGCCACACCCACTCCCCTCTGCACCACCC CGCCCAGCCCAGCATACCACCATGGCCACCAGGTCTCCAGCTCTGCCCCCAGAGACCCCAGCTGCCGCCA GCCTGTCAACAGCCACTGATGGGCTGGCAGCCACACCCTTCATGTCCCTTGAGTCAACTCGTCCCTCCCA GCTCCTCTCTGGCCTGCCTCCCGACACCAGCCTGCCCCTGGCCAAGGTGGGCACATCTGCCCCAGTGGCC ACACCCGGCCCCAAAGCCTCTGTCATCACCACTCCACTCCAGCCACAGGCCACGACTCTGCCTGCTCAGA CACTTAGCCCAGTACTGCCTTTCACTCCAGCAGCAATGACCCAGGCGCACCCACCCACTCACATAGCACC CCCAGCAGCAGGCACAGCTCCAGGCCTGCTGCTGGGAGCCACATTGCCAACCTCTGGAGTCCTGCCTGTG GCTGAGGGCACGGCCTCCATGGTATCTGTTGTCCCACGAAAGAGCACCACAGGGAAGGTGGCCATCCTAT CCAAGCAAGTGTCTCTGCCCACTTCCATGTATGGTTCTGCAGAGGGTGGGCCCACAGAGCTCACGCCTGC TACGAGCCACCCTCTCACGCCCTTGGTGGCTGAGCCCGAGGGAGCCCAGGCAGGCACAGCTCTGCCAGTG CCCACATCCTATGCCCTGAGCCGTGTCTCAGCCAGGACGGCCCCCCAAGACAGCATGCTGGTTCTGTTGC CTCAGCTGGCTGAGGCCCATGGAACCTCGGCAGGGCCTCACCTGGCAGCAGAGCCGGTGGACGAGGCCAC CACAGAACCATCTGGGCGCTCAGCCCCAGCCCTGAGCATCGTAGAGGGTTTGGCGGAGGCTTTGGCAACT ACCACTGAGGCCAATACATCCACCACCTGTGTTCCAATCGCCGAGCAGGACTGCGTCCGCCACATCTGCC TGGAGGGCCAGCTGATTCGCGTGAATCAGTCCCAGCACTGTCCCCAGGGTGCTGCTCCCCCTCGCTGTGG GATCCTGGGCCTCGCCGTGCGGGTGGGTGGGGACCGCTGCTGCCCACTCTGGGAGTGTGCCTGCCGGTGC TCAATCTTCCCTGACCTGAGCTTCGTGACCTTCGATGGGAGCCACGTAGCTCTGTTCAAGGAGGCCATCT ACATCCTCAGCCAGAGCCCAGATGAAATGCTCACCGTCCATGTACTGGACTGCAAAAGTGCCAACCTGGG GCACCTGAACTGGCCCCCGTTCTGTCTGGTGATGTTGAACATGACTCACTTGGCCCATCAGGTCACTATT GATCGCTTCAACCGAAAGGTGACTGTGGACTTGCAGCCTGTGTGGCCACCGGTGAGCAGGTATGGATTCA GAATTGAGGACACAGGCCACATGTACATGATCCTGACTCCCTCAGACATCCAGATCCAGTGGCTCCACAG CTCAGGACTCATGATCGTGGAGGCCAGCAAAACCAGCAAGGCCCAGGGCCATGGCCTGTGCGGTATCTGT GATGGAGATGCAGCCAATGACCTTACCCTGAAGGATGGCTCAGTGGTGGGTGGGGCTGAGGACCCTGCTC CCTTTCTGGACAGCTGGCAGGTGCCCAGCTCCCTGACCTCAGTGGGCCAGACCCGCTTCCGCCCAGACAG CTGCGCCACAACTGACTGCTCGCCCTGCCTTCGCATGGTGTCCAACCGCACCTTCAGTGCCTGCCACCGC TTTGTGCCTCCGGAGTCATTCTGTGAGCTGTGGATCCGGGACACCAAGTACGTGCAGCAGCCCTGCGTGG CCCTGACTGTGTACGTGGCCATGTGCCACAAATTTCATGTGTGCATCGAGTGGCGGCGCTCTGACTACTG CCCCTTCCTGTGCTCCAGCGACTCCACATACCAGGCATGTGTGACAGCCTGTGAGCCACCCAAGACATGC CAGGATGGGATACTAGGGCCTCTGGACCCAGAGCACTGCCAGGTGCTGGGCGAGGGCTGCGTCTGCTCCG AGGGCACCATCTTACACCGGCGCCACTCTGCACTCTGCATCCCGGAGGCCAAGTGCGCCTGCACTGACAG CATGGGGGTGCCGAGGGCCCTGGGGGAGACCTGGAACAGCTCCCTCAGCGGCTGCTGCCAGCACCAGTGC CAAGCCCCAGACACCATTGTCCCGGTGGATCTGGGCTGCCCCAGTCCCCGCCCTGAGAGCTGCCTGCGAT TCGGGGAGGTGGCCTTGCTCCTACCCACCAAGGACCCCTGCTGCCTGGGGACTGTCTGTGTGTGTAACCA GACTCTGTGTGAGGGTCTCGCCCCCACATGCCGCCCAGGCCACCGCCTCCTCACCCACTTCCAGGAGGAC TCCTGCTGCCCCAGCTACAGCTGTGAGTGTGACCCAGATCTCTGTGAGGCAGAGCTGGTCCCCAGCTGCC GACAGGACCAGATCCTGATCACGGGCCGCCTGGGGGACTCCTGCTGCACCTCCTACTTCTGCGCCTGTGG TGACTGTCCAGACTCCATCCCCGAATGTCAAGAAGGGGAGGCGCTCACTGTGCACAGGAATACCACGGAA CTCTGCTGCCCTCTGTACCAGTGTGTGTGTGAGAACTTCCGCTGTCCCCAAGTGCAGTGTGGCCTGGGCA CTGCCCTGGTGGAGGTGTGGAGCCCCGACCGCTGCTGCCCCTACAAATCCTGTGAATGTGACTGTGACAC AATCCCGGTGCCCCGGTGCCATCTGTGGGAGAAATCCCAGCTGGATGAGGAGTTCATGCACAGCGTGGAG AATGTGTGTGGCTGCGCCAAGTACGAGTGTGTGAAGGCCCCGGTGTGTCTGAGCCGCGAGCTGGGTGTGA TGCAGCCCGGCCAGACAGTGGTGGAGCTCTCAGCAGATGGCGTGTGCCACACCTCCCGCTGCACCACCGT GCTCGACCCTCTCACCAACTTCTACCAGATCAACACCACCTCCGTGCTCTGTGACATCCACTGTGAGGCG AACCAGGAGTACGAGCACCCGCGGGACCTCGCTGCCTGCTGCGGCTCCTGCAGGAACGTGTCCTGTCTCT TCACCTTCCCCAATGGCACCACCTCCCTGTTCTTGCCCGGGGCATCCTGGATCGCAGACTGCGCCCGCCA CCACTGCAGCAGCACGCCCCTGGGTGCCGTGCTGGTCCGCTCTCCCATAAGCTGCCCACCGCTCAATGAG ACTGAGTGTGCCAAGGTTGGGGGTTCCGTGGTACCTTCCTTGGAAGGATGCTGCAGGACCTGTAAGGAGG ATGGGCGCTCCTGCAAGAAGGTGACCATCCGCATGACCATCCGCAAGAATGAATGCAGGAGCAGCACCCC TGTGAACCTAGTGTCCTGCGATGGGAGGTGCCCATCCGCCAGCATCTACAACTACAACATCAACACCTAT GCCCGATTCTGCAAGTGCTGCCGTGAGGTGGGCCTGCAGCGGCGCTCTGTGCAGCTCTTCTGTGCCACCA ATGCCACCTGGGTGCCCTATACAGTGCAGGAGCCCACCGACTGTGCCTGCCAGTGGTCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001292063
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001292063.1, NP_001278992.1
RefSeq Size	8742 bp
RefSeq ORF	8742 bp
Locus ID	340990
Gene Summary	The protein encoded by this gene is a component of the acellular membranes of the inner ear. Disruption of the orthologous mouse gene shows that it plays a role in auditory and vestibular functions. It is involved in fibrillar network organization, the anchoring of otoconial membranes and cupulae to the neuroepithelia, and likely in sound stimulation resistance. Mutations in this gene cause autosomal recessive nonsyndromic deafness, type 18B. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014] Transcript Variant: This variant (2) uses two alternate splice sites and includes an additional exon, which together result in an alternate in-frame segment in the 5' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The exon combination of this transcript is inferred from the full-length mouse mRNA U96411.1, and is supported by partial human transcript alignments, RNA-seq data, and partial RT-PCR data from PMID:23122587. CCDS Note: This CCDS representation lacks full-length human transcript support. Its exon combination is therefore inferred, but it is supported by the full-length orthologous mouse transcript U96411.1, by partial human transcript alignments, and by data in PMID:23122587. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

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