NSMP-1, non-structural mature protein 1 (NC_001943) Virus Tagged ORF Clone
CAT＃: VC100539
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for NSMP-1, non-structural mature protein 1 [Human astrovirus], codon optimized for human cell expression, NP_751902
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CNY 3800.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC100539 represents NCBI reference of NP_751902 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCCTACGGGGAACCATACTATTCATCAAAACCAGATAAAGATTTCAATTTCGGCAGTACCATGG CAAGGCGGCAAATGACCCCAACGATGGTCACCAAACTTCCCAAATTCGTCCGAAACTCACCACAGGCATA CGACTGGATTGTGAGGGGGCTCATCTTTCCCACTATTGGAAAGACATACTTCCAGCGGGTGGTCGTCATT ACCGGCGGCCTGGAAGATGGAACTTACGGGTCCTTCGCCTTTGATGGCAAGGAGTGGGTTGGAATTTACC CAATTGAACACCTCAACTTGATGTCTTCTCTGAAGCTGATCCACAAGGCGAACGCCCTCCAGGAGCGCTT GCGGCTGTCTCAAGAAGAGAAGGCGACCTTGGCCCTCGACGTTCAGTTTCTCCAGCATGAGAATGTTCGC CTGAAAGAGATGATCCCAAAGCCTGAACCCAGAAAAATACAGATGAAATGGATTATTATGGGAGCAGTTC TGACTTTCCTCTCATTGATTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100539 representing NP_751902
Red=Cloning sites Green=Tags MMAYGEPYYSSKPDKDFNFGSTMARRQMTPTMVTKLPKFVRNSPQAYDWIVRGLIFPTIGKTYFQRVVVI TGGLEDGTYGSFAFDGKEWVGIYPIEHLNLMSSLKLIHKANALQERLRLSQEEKATLALDVQFLQHENVR LKEMIPKPEPRKIQMKWIIMGAVLTFLSLIP TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001943 |
| ORF Size | 513 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001943.1, NP_751902 |
| RefSeq ORF | 513 bp |
| MW | 19.7 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC100534 | Myc-DDK-tagged ORF clone of viral ORF for capsid precursor protein [Human astrovirus], codon optimized for human cell expression, NP_059444 |
CNY 11970.00 |
|
| VC100536 | Myc-DDK-tagged ORF clone of viral ORF for VP34 precursor [Human astrovirus], codon optimized for human cell expression, NP_751899 |
CNY 3800.00 |
|
| VC100537 | Myc-DDK-tagged ORF clone of viral ORF for VP29 precursor [Human astrovirus], codon optimized for human cell expression, NP_751900 |
CNY 4660.00 |
|
| VC100538 | Myc-DDK-tagged ORF clone of viral ORF for VP26 precursor [Human astrovirus], codon optimized for human cell expression, NP_751901 |
CNY 5230.00 |
|
| VC100540 | Myc-DDK-tagged ORF clone of viral ORF for NSMP-3, non-structural mature protein 3 [Human astrovirus], codon optimized for human cell expression, NP_751903 |
CNY 3800.00 |
|
| VC100541 | Myc-DDK-tagged ORF clone of viral ORF for NSMP-4, non-structural mature protein 4 [Human astrovirus], codon optimized for human cell expression, NP_751904 |
CNY 3800.00 |
|
| VC100542 | Myc-DDK-tagged ORF clone of viral ORF for orf1b product [Human astrovirus], codon optimized for human cell expression, NP_751905 |
CNY 6270.00 |
|
| VC100543 | Myc-DDK-tagged ORF clone of viral ORF for NSMP-2, non-structural mature protein 2 [Human astrovirus], codon optimized for human cell expression, NP_751906 |
CNY 3800.00 |
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