PB1 (NC_006503) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC102286 represents NCBI reference of YP_145804 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCCTGGTCGGAGGCCTCCTCACTGGAGAGGACAGCCTGATTTCCATGTCCAACGACGTGTCCT GCCTCTACGTCTACGACGGGCCCATGAGAGTCTTCAGCCAGAACGCCCTGATGCCAACCCTCCAATCCGT CAAACGGTCCGACCAGTTCAGCAAGGGCAAGACAAAACGGTTCATCATTGATCTGTTCGGAATGAAACGG ATGTGGGACATCGGCAACAAGCAACTGGAGGATGAGAACCTGGATGAAACAGTGGGAGTGGCCGACCTGG GCCTCGTCAAGTACCTCATCAACAACAAGTACGATGAAGCCGAGAAGACAAGCCTGCGGAAGTCCATGGA GGAGGCCTTTGAGAAGTCCATGAACGAAGAGTTCGTGGTCCTGAACAAAGGCAAGTCCGCCAACGACATC ATTTCCGACACCAACGCCATGTGCAAGTTTTGCGTCAAAAACTGGATCGTCGCCACCGGGTTTCGGGGCC GGACCATGAGCGACCTCATCGAGCACCACTTCCGGTGCATGCAGGGCAAGCAGGAGGTCAAGGGCTACAT CTGGAAGCACAAGTACAACGAGCGGCTGAAGCGGAAGCAGCTGTCCAAAGAGGAGGTCAAGTTCGACCGG GAGGAGTACACCAGCCGGTCCTTCCGGCTGCTCAGCTTCCTGAAAAACAGCGAACGGACCAAGCTGGAGC CTCGGGCCGTGTTCACCGCTGGCGTGCCTTGGCGGGCCTTCATCTTCGTCCTCGAGCAAACAATGCTGGT CGTGAACAAGCTGGACCCCAACTCCGTGATTTGGATGGGCAGCGACGCCAAGATCAACACCACCAACAGC CGGATCAAGGAGATCGGCATGAAGAACCAGGGGCAAACCCTCGTGACCCTGACCGGCGACAACAGCAAGT ACAACGAGAGCATGTGCCCAGAGGTGATGATGGTGTTCCTCCGGGAGCTGGGCATCAAGGGACCCATGCT CGAGGTGCTGGACTACGCCCTCTGGCAGTTCAGCCAGAAGTCCGTCAAGCCCGTGGCCCCCATCAAGAAG CGGACCGGCAAAAGCACCGTGGTGATCAAGGCCGACAGCGTGAAGGAGTGCCGGGACGCCTTCAACGAAA AGGAGCTCGAGCTGATCCAGGGCGTGGAGTGGATGGACGACGGCTTCGTGCGGGTCCGGCGGGGCATGCT GATGGGCATGGCCAACAACGCCTTCACTACCGCCAGCACCATCGCCAGCAGCTTCAGCTTCACCCCCGAG GCAGTGTACACCCTGCAGAGCAGCGACGACTTCGTCACCGGCTCCTGCGGACGGGACGTGCAGCACGCCC GGCAGCGGCTCGAGATGGCCCTCAAGGTCAGCAAGGCCGCCGGCCTGAATGTCAGCCAGAAAAAGAGCTT CTACGTCGAGGGCACCACATTCGAGTTCAACTCCATGTTCGTGCGGGACGGGAAGGTGATGGCCAACGGC GGCAACTTCGAAAACATGACCGTGCCCGGGGGCCTCGGCCCCTCCACCGACCTGTTCGTCGTGGGCAAGC AGGCCCGGAACAGCATGCTGCGGGGCAACCTGAGCTTCAGCCAGGCCATGGAGATGTGCAAGATCGGCAT CACCAATGTCGAAAAGGTGTACTATGGGAACCGGAAGTACCAGGAGCTGAAGAACGAGATTCGGGAGAAG TGCGGCGAGGAGACCATGAGCATCCCCGAATCCATGGGCGGCGACCGGAAGCCCCGGCCCTGGGAGCTCC CCCAGAGCTTCGACGGCATCGCCCTCAAGGAAGCCGTCAACCGGGGCCACTGGAAGGCCGCCAAGTACAT CAAATCCTGCTGCAGCATCGAGTTCGACGAGGAGGGCGACCAGTCCTGGGACACAAGCAAGACCGCCCTG GTCGTGATCCGGAAGAACGAAACCGACATGCGGCGGCGGACCGTGAAGACCCGGAACCCCAAGGACAAGA TCTTCAACGACGCCATGAACAAGGCCAAGCGGATGTACGAAACCGTGGTGGACCGGAACCCCCTGCTGGG CCTCAAGGGGAAAGGCGGACGGCTCACCGTCAAGGACCTGAAAGCCAGAAAGCTGATCGACGAGGTCGAG GTGGTCAAGAAGAAGAAGCACGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC102286 representing YP_145804 Red=Cloning sites Green=Tags METLVGGLLTGEDSLISMSNDVSCLYVYDGPMRVFSQNALMPTLQSVKRSDQFSKGKTKRFIIDLFGMKR MWDIGNKQLEDENLDETVGVADLGLVKYLINNKYDEAEKTSLRKSMEEAFEKSMNEEFVVLNKGKSANDI ISDTNAMCKFCVKNWIVATGFRGRTMSDLIEHHFRCMQGKQEVKGYIWKHKYNERLKRKQLSKEEVKFDR EEYTSRSFRLLSFLKNSERTKLEPRAVFTAGVPWRAFIFVLEQTMLVVNKLDPNSVIWMGSDAKINTTNS RIKEIGMKNQGQTLVTLTGDNSKYNESMCPEVMMVFLRELGIKGPMLEVLDYALWQFSQKSVKPVAPIKK RTGKSTVVIKADSVKECRDAFNEKELELIQGVEWMDDGFVRVRRGMLMGMANNAFTTASTIASSFSFTPE AVYTLQSSDDFVTGSCGRDVQHARQRLEMALKVSKAAGLNVSQKKSFYVEGTTFEFNSMFVRDGKVMANG GNFENMTVPGGLGPSTDLFVVGKQARNSMLRGNLSFSQAMEMCKIGITNVEKVYYGNRKYQELKNEIREK CGEETMSIPESMGGDRKPRPWELPQSFDGIALKEAVNRGHWKAAKYIKSCCSIEFDEEGDQSWDTSKTAL VVIRKNETDMRRRTVKTRNPKDKIFNDAMNKAKRMYETVVDRNPLLGLKGKGGRLTVKDLKARKLIDEVE VVKKKKHV TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_006503
ORF Size	2124 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_006503.1, YP_145804
RefSeq ORF	2124 bp
Locus ID	3170817
MW	80.5 kDa

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