Insulin-like growth factor I / IGF1 Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MGPETLCGAE LVDALQFVCG DRGFYFNKPT GYGSSSRRAP QTGIVDECCF RSCDLRRLEM YCAPLKPAKS A
Predicted MW	7.7 kDa
Concentration	lot specific
Purity	>95% pure by SDS-PAGE
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: PBS, pH 7.4
Preparation	Liquid purified protein
Protein Description	Recombinant Human IGF-1 was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: The ED50 for this effect is < 3.6 ng/ml. Measured in a cell proliferation assay using MCF7 cell.
Reference Data
RefSeq	NP_000609
Locus ID	3479
UniProt ID	P05019
Cytogenetics	12q23.2
Synonyms	IGF-I, Somatomedin-C, Mechano growth factor, MGF, IBP1
Summary	The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	Dilated cardiomyopathy, Focal adhesion, Glioma, Hypertrophic cardiomyopathy (HCM), Long-term depression, Melanoma, mTOR signaling pathway, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer

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