AKR1B1 / ALDR1 (1-316) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF
Predicted MW	35.8 kDa (316aa) confirmed by MALDI-TOF
Concentration	1mg/ml (determined by Bradford assay)
Purity	> 95% by SDS-PAGE
Buffer	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Preparation	Liquid purified protein
Applications	SDS-PAGE, Enzyme Activity
Protein Description	Recombinant Aldose reductase (AKR1B) protein was expressed in E.coli and purified by using conventional chromatography techniques.
Storage	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability	Shelf life: one year from despatch.
Bioactivity	Specific activity is >800pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.
Endotoxin	< 1 EU per 1ug of protein (determined by LAL method)
Reference Data
RefSeq	NP_001619.1
Locus ID	231
UniProt ID	P15121
Cytogenetics	7q33
Synonyms	Aldo-keto reductase family1 member B1, AKR1B1, ADR, ALDR1, ALR2, AR, Aldose reductase, AKR1B 1, Aldehyde reductase, aldr 1, aldr-1, Lii5-2 CTCL tumor antigen, low Km aldose reductase
Summary	This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]
Protein Families	Druggable Genome
Protein Pathways	Fructose and mannose metabolism, Galactose metabolism, Glycerolipid metabolism, Metabolic pathways, Pentose and glucuronate interconversions, Pyruvate metabolism

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