AKR1B10 (1-316) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY
Concentration	lot specific
Purity	>95% pure by SDS-PAGE
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol
Preparation	Liquid purified protein
Protein Description	Recombinant Human AKR1B10 protein was expressed in E.coli and purified by using conventional chromatography.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: Approximately 0.32-0.4 units/mg protein. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C. Activity Assay 1. Prepare a 1.0 ml reaction mix into a suitable container: The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50 ul of recombinant AKR1B10 solution with various concentrations (2.5ug, 5ug, 10ug) in 750 ul reaction buffer. 3. Mix by inversion and incubate at 25°C for 2.5 minutes. 4. Add 200 ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase in A340nm for 3 minutes.
Reference Data
RefSeq	NP_064695
Locus ID	57016
UniProt ID	O60218
Cytogenetics	7q33
Synonyms	AKR1B11, Aldose reductase-like, ARL-1
Summary	This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome
Protein Pathways	Butanoate metabolism, Fructose and mannose metabolism, Linoleic acid metabolism, Metabolic pathways

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