D-Dimer Mouse Monoclonal Antibody [Clone ID: DD93]

Specifications

Product Data
Clone Name	DD93
Applications	ELISA
Recommend Dilution	Immunoassays for the quantitative determination of D-dimer and high molecular weight fibrin degradation products. All antibodies recognize D-dimer in ELISA. All MAbs recognize D-dimer in Western blotting under non-reducing conditions. MAbs DD22, DD41, DD44, DD46 and DD189 interact with beta-chain of D-dimer in Western blotting under reducing conditions. MAbs DD93 and DD255 interact with gamma-chain of D-dimer in Western blotting under reducing conditions. Recommended pairs to be used in a sandwich immunoassay for D-dimer detection in human plasma (capture-detection): One-step sandwich immunoassays: DD189 – DD255 (Equal specificity to D-dimer and high MW fibrin degradation products). DD2 – DD41 (Slightly more specific to high MW fibrin degradation products at high concentrations). Two-step sandwich immunoassays*: DD2 – DD4 (Approximately equal specificity to D-dimer and high MW fibrin degradation products). (*To be analyzed in a two-step sandwich immunoassay, plasma must be diluted 1:1 or more with 10 mM Tris-HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding the final NaCl concentration in plasma samples must be 0.5 M or more.)
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with homogenized fibrin clot, D-dimer or high molecular weight fibrin degradation products.
Specificity	All MAbs recognize D-dimer and high molecular weight fibrin degradation products. MAb DD93 recognizes a cross-linked region of D-dimer. MAbs DD1, DD2, DD3, DD22, DD41, DD44, DD46, DD93, DD189 and DD255 do not cross-react with fibrinogen. MAbs DD4, DD5 and DD6 show cross-reaction with fibrinogen.
Isotype	IgG1
Formulation	PBS, pH 7.4 State: Purified Preservative: 0.1% Sodium Azide
Purification	Chromatography on protein A Sepharose
Conjugation	Unconjugated
Storage Condition	Store at 4°C.
Synonyms	Fibrin breakdown product, Fibrinogen Degradation Product D
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.