囊泡/外泌体提取试剂——快速、高效且无需超速离心
EV Isolation Reagent

OriGene的EV分离试剂采用聚合物沉淀技术，为从细胞培养介质中分离细胞外囊泡（EVs）提供了超速离心和色谱法的强大替代方案。

产品优势

  • 快速简易的操作流程：无需超速离心机，2小时内即可完成EVs分离
  • 高回收率：采用温和聚合物沉淀技术，实现更高效EVs提取
  • 灵活可扩展：单个试剂盒可处理0.5mL至200mL不同体积样本
  • 下游应用兼容：适用于蛋白质、RNA、脂质及颗粒分析
  • 缓冲液澄清技术：预澄清缓冲液无背景颗粒/聚集体，确保纯净EV分析
  • 结果可重复：多种样本类型均能获得稳定的EVs产量
  • 经济高效：与超速离心/尺寸排阻色谱相比更具成本效益
货号 产品描述 价格 货期
AR100029S EV Isolation Reagent, 15 mL ￥1500 4周
AR100029 EV Isolation Reagent, 50 mL ￥2800 4周

产品性能检测

1.形态鉴定及理化性质检测

Figure 1A. Nanoparticle tracking analysis (NTA) demonstrated significantly higher EV recovery (>10-fold) using OriGene's EV isolation reagent compared to ultracentrifugation. EVs were isolated from HEK293 cell culture supernatants.
Figure 1B. BCA and Western blot analysis of EVs from stem cell conditioned medium showed that OriGene's EV isolation reagent produced higher EV yields than Vendor Q, confirmed by stronger CD81 signals and greater protein content.
Figure 2. EVs isolated from HeLa cells using OriGene's EV isolation reagent showed good stability by Zeta potential analysis, with size and integrity comparable to ultracentrifugation, confirming their high quality.

2.标记囊泡的摄取：细胞摄取实验

Figure 3. GFP-tagged EVs from a stable CD63-GFP cell line were isolated using OriGene's EV isolation reagent and co-cultured with naïve SH-SY5Y cells. After 72 hours, efficient, dose-dependent uptake confirmed their functional delivery capability.

3.EV标志物蛋白验证

Figure 4. Simple Western analysis of HEK293T-derived EVs isolated using the OriGene's EV isolation reagent showed clear detection of EV markers using CD63 mouse mAb (SKU: TA803474), TSG101 mAb (SKU: TA385476), CD81 mAb, and MFGE8 mAb (SKU: TA809279). EVs were diluted to 0.2 mg/ml (protein equivalence) and analyzed with the Jess system. Jess Simple Western system.

外泌体囊泡 全流程研究解决方案

Isolation
  • Precipitation
    Method

    • Polymer-based
    (NEW!)
Detection
  • EV Markers

    • Antibodies against general EV markers Full-length recombinant EV marker proteins for use as a standard in immunoassays
Cargo Analysis
Functional Analysis

产品相关资料