alpha 1 Catenin (CTNNA1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 15D9]

Specifications

Product Data
Clone Name	15D9
Applications	ELISA, IF, IP, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from untreated A431 cells. ELISA: 0.05 µg/ml. Immunoprecipitation: 1-10 µg per 10e6 vanadate-treated A431 cells. Immunocytochemistry: 0.1-1 µg/ml. Mab Anti-Catenin Clone 15D9 may tolerate formaldehyd. Positive Control Cell Lysate: A431 Untreated Format: Lyophilized cell lysate from serum starved A431 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant α-Catenin
Specificity	This antibody specifically recognizes α-Catenin in Western blot at 102 kDa.
Isotype	IgG1
Formulation	1 ml 2x PBS / 0.09% Sodium Azide / PEG and Sucrose State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	catenin alpha 1
Database Link	Entrez Gene 1495 Human UniProt ID P35221
Background	The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The N-terminus of α-catenin directly interacts with exon 3 of β-catenin. The C-terminus of α-catenin interacts with the actin filament network.
Synonyms	Alpha E-catenin, Alpha Catenin
Reference Data

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