TPTEP2-CSNK1E (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 4D7]

Specifications

Product Data
Clone Name	4D7
Applications	ELISA, WB
Recommend Dilution	ELISA: 0.1 µg/ml. Western blot: Use at 0.5 µg/ml for HRPO/ECL detection. Detects a band at 45kD. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from Untreated A431 cells.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide (aa 355 - 380 of CK1e) conjugated to hemocyanin
Specificity	Mab CK1e-4D7 specifically recognizes the epsilon isoform of CK1.
Isotype	IgG1
Formulation	1ml 2xPBS containing 0.09% Sodium Azide, PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Reconstitute with 1 ml H2O (15 min, RT)
Concentration	lot specific
Purification	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	TPTEP2-CSNK1E readthrough
Database Link	Entrez Gene 102800317 Human UniProt ID P49674
Background	Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as subtrates. It can also phosphorylate a large number of proteins and participates in Wnt signaling.
Synonyms	CKI-epsilon, CKIe, CSNK1E
Note	The lyophilized cell lysates (see below) contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation. Protocol: Positive Control: Cell lysate from Untreated A431 cells. Format: Lyophilized cell lysate from Serum starved A431 cells Reconstitution: Restore by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 μl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 μl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.
Reference Data

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