MEK7 (MAP2K7) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10F7]

CAT#: AM00096PU-N

MEK7 (MAP2K7) (incl. pos. control) mouse monoclonal antibody, clone 10F7, Purified



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CNY 5,973.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 10F7
Applications ELISA, IF, IHC, WB
Recommend Dilution ELISA: 0.1 µg/ml.
Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Included Positive Control: Cell lysate from Untreated SW480 cells.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivity Canine, Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Immunogen Synthetic peptide conjugated to KLH. 
Epitope: N-Terminus.
Specificity This antibody specifically recognizes the N-terminus of MKK7.
Formulation 1 ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose
State: Purified
State: Lyophilized purified IgG fraction from Serum-free Cell Culture Supernatant
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Size Exclusion Chromatography
Conjugation Unconjugated
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Predicted Protein Size 45 kDa
Gene Name mitogen-activated protein kinase kinase 7
Background The stress response of mammalian cells results in activation of kinases of the SAPK1 and SAPK2 families. The SAP kinases are specifically activated by dual phosphorylation of a conserved T-X-Y motif by MAP kinase kinases (MKK). The 3 isoforms of the SAPK1 family (SAPK1α/jnk2; SAPK1β/jnk3; SAPK1γ/jnk1) are synergistically activated by phosphorylation of the threonine residue by MKK7 and the tyrosine residue by MKK4.
Synonyms MAPK/ERK kinase 7, JNK-activating kinase 2, c-Jun N-terminal kinase kinase 2, MAPKK 7, NKK2, MEK7, MKK7, MAP kinase kinase 7
Note Protocol: Positive Control Cell lysate from untreated SW480 cells

Format: Lyophilized cell lysate from serum starved SW480 cells.

Reconstitution:
Restore by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDSPAGE
sample buffer, mix and incubate at 90°C for 5 min.

Applications:
The positive control cell lysate is recommended for immunoblot applications.
20 µl of positive control cell lysate correspond to ca. 20.000 cells.
Use 20 μl / lane (mini gel) for HRPO/ECL detection of the target proteins.
Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.

Storage:
In aliquots at -20°C. Avoid repeated freezing and thawing.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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