eNOS (NOS3) pSer1177 Mouse Monoclonal Antibody [Clone ID: 15E2]

Specifications

Product Data
Clone Name	15E2
Applications	ELISA, WB
Recommend Dilution	ELISA: Use at 0.1 µg/ml. Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Provided Positive Control: Cell lysate from pervanadate treated Bend3 cells (See Protocol).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH. Epitope: Phosphoserine 1177
Specificity	This antibody specifically recognizes eNOS phosphorylated at Serine 1177.
Isotype	IgG1
Formulation	PBS State: Purified State: Liquid purified IgG fraction Stabilizer: 50% Glycerol, PEG and Sucrose Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	nitric oxide synthase 3
Database Link	Entrez Gene 4846 Human UniProt ID P29474
Background	Endothelial NO synthase (eNOS) produces nitric oxid (NO) which plays a crucial role in cardiovascular homeostasis. Activation of the enzyme and NO production can be induced by phosphorylation of eNOS at serine 1177 by activated Akt/PKB.
Synonyms	Endothelial NOS, EC-NOS, NOS type III, Constitutive NOS, eNOS
Note	Protocol: Positive Control: Bend 3 pervanadate treated Format: Lyophilized cell lysate from Bend 3 cells. Serum starved cells were treated for 15 min. with pervanadate Reconstitution: Restore by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 μl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 μl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing
Reference Data

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