Bovine Corona Virus E2 protein Mouse Monoclonal Antibody [Clone ID: 5A4]

Specifications

Product Data
Clone Name	5A4
Applications	ELISA
Recommend Dilution	ELISA. HIT (hemagglutination inhibition titer).
Reactivity	Bovine Coronavirus
Host	Mouse
Clonality	Monoclonal
Immunogen	Bovine corona virus. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Specificity	Recognizes bovine corona virus surface antigen (peplomer).
Isotype	IgG1
Formulation	PBS, pH 7.4 containing 0.09% Sodium azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	>95% pure (SDS-PAGE). Protein G chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Database Link	UniProt ID P25193
Background	Bovine Corona Virus Spike glycoprotein is cleaved into S1 and S2 Spike proteins. The precursor is processed into S1 and S2 by host cell furin or furin-like protease to yield the mature S1 and S2 proteins. The cleavage site between S1 and S2 requires the optimal sequence [KR]-X-[KR]-R. S1 attaches the virion to the cell membrane by binding to 9-O-acetylated sialic acid containing proteins, initiating the infection. S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Synonyms	Spike glycoprotein, S glycoprotein, Peplomer protein, E2 protein
Note	Centrifuge before opening to ensure complete recovery of vial contents.
Reference Data

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