Heparan-Sulfate-Proteoglycan Mouse Monoclonal Antibody [Clone ID: 1F10/B8]

Specifications

Product Data
Clone Name	1F10/B8
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA (less than 1 µg/ml). Western blot (1 µg/ml). Immunohistochemistry (cryosections, 1 µg/ml).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Human small basement membrane Heparan Sulfate Proteoglycan (HS-PG)
Specificity	This Monoclonal antibody 1F10/B8 is specific for a core protein epitope of a Human small basement membrane Heparan Sulfate proteo-glycan (HS-PG). AM02102PU recognizes an epitope different from that recognized by clones 2H7/G11 (Cat.-No AM02103PU) and 2E2/B5 (Cat.-No AM20231PU).
Isotype	IgG1
Formulation	50 mM TRIS pH 7.4 State: Purified State: Lyophilized purified IgG fraction from Cell Culture Supernatant
Reconstitution Method	Restore in aqua bidest to 1 mg/ml.
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background	The principal molecular structure of basement membranes has been elucidated during the past two decades and it was shown that for example in the glomerular basement membrane (GBM) HS-PG are responsible for the selective filtration process. Removal of HS-PG will lead to proteinuria. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that (at least two) other basement membrane HS-PG do exist: Agrin, originally discovered as an important component of the neuromuscular junction and a novel small HS-PG, that was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200 kDa (aorta) and 30-160 kDa (kidney) and a core protein size of 24 kDa or 22 kDa, respectively, was localized by immunohisto-chemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.
Synonyms	small Heparan sulfate proteoglycan core protein
Note	Protocol: 1. Stöcker G, Meyer HE, Wagener C and Greiling H (1991): Purification and N-terminal amino acid sequence of a chondroitin sulfate / dermatan sulfate proteoglycan isolated from intima / media preparations of human aorta. Biochem J 274: 415-420. 2. Heintz B, Stöcker G, Rentz U, Melzer H, Mrowka C, Stickeler E, Sieberth HG, Greiling H, Haubeck HD (1995): Decreased glomerular basement membrane heparan sulfate proteoglycan in essential hypertension. Hypertension 25: 399-407. 3. Stefanidis I, Heintz B, Stöcker G, Mrowka C, Sieberth HG, Haubeck HD (1996): Association between heparan sulfate proteoglycan excretion and proteinuria after renal transplantation. J Am Soc Nephrol 7: 1-7. 4. Stöcker G, Stickeler E, Switalla S, Fischer DC, Greiling H, Haubeck HD (1997): Development of an enzyme immuno assay specific for a core protein epitope of a novel small basement membrane associated heparan sulphate proteoglycan from human kidney. Eur J Clin Chem Clin Biochem 35: 95-99.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.