PLSCR1 Mouse Monoclonal Antibody [Clone ID: 13A6 [TEC-23]]

Specifications

Product Data
Clone Name	13A6 [TEC-23]
Applications	IF, IP
Recommend Dilution	Immunoprecipitation. Western Blotting: 1 μg/ml. Flow Cytometry. Immunocytochemistry.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	BALB/c mice were immunized with pooled lipid rafts isolated from RBL-2H3 cells. Hybridoma cells were obtained after fusion of SP02 mouse myeloma cells with spleen cells of immunized mice using standard procedures.
Specificity	This antibody binds to rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 kDa membrane-associated protein accelerating bidirectional movement of plasma membrane phospholipids during conditions of elevated calcium.
Isotype	IgG2a
Formulation	State: Ascites State: Liquid Undiluted Ascites Preservative: 15 mM Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Background	PLSCR1 (Phospholipid Scramblase 1) is a multiply palmitoylated endofacial plasma membrane protein containing several SH3 and WW domain binding motives. In the plasma membrane, PLSCR1 plays a role in transbilayer lipid redistributions and signal transduction. Nonpalmitoylated PLSCR1, however, is able to be transported into the nucleus and bind DNA. PLSCR1 potentiates the antiviral activity of interferon and its expression is highly induced by interferons and growth factors.
Synonyms	Phospholipid Scramblase 1
Note	Protocol: Western Blot: Positive control: RBL Rat basophilic leukemia cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% Triton X-100 in 20 mM Tris/Cl, 100 mM NaCl, 2 mM EDTA, 2 mM Na3Vo4, 10 mM glycerolphosphate, pH 8.0, 1 mM PMSF, 10 μg/ml aprotinin). Incubate 20 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min. Note: Both reducing and non-reducing conditions.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.