CD73 (NT5E) (27-252) Mouse Monoclonal Antibody [Clone ID: AT89E9]

Specifications

Product Data
Clone Name	AT89E9
Applications	ELISA, FACS, FC, ICC/IF, IF, IHC, WB
Recommend Dilution	ELISA. Flow Cytometry. Western blot: 1/1,000-1/2,000. Immunofluorescence/Immnunocytochemistry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human CD73 (27-252aa) purified from E. coli
Specificity	This antibody is specific for Human NT5E/CD73. Other species not tested.
Isotype	IgG2b, kappa
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration	1mg/ml (determined by BCA assay)
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Gene Name	5'-nucleotidase ecto
Database Link	NP_002517 Entrez Gene 4907 Human UniProt ID P21589
Background	CD73 (Ecto-5-nucleotidase, 5-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.
Synonyms	CD73, 5-NT, 5'-nucleotidase, 5'-nucleotidase ecto, AI447961, E5NT, ecto, eN, eNT, NT, NT5, Nt5e, NTE, 2210401F01Rik
Reference Data

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