dam Mouse Monoclonal Antibody [Clone ID: RB1]

Specifications

Product Data
Clone Name	RB1
Applications	WB
Recommend Dilution	Western Blot: 5-10 µg/ml.
Reactivity	Bacteria
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant DNA Adenine Methylase protein.
Specificity	Gram Negative Bacteria.
Isotype	IgG1
Formulation	PBS containing 0.08% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Database Link	UniProt ID P0AEE8
Background	DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence); (ii) methyl-directed mismatch repair; (iii) control of transposition of certain transposable DNA elements; and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science; 284:967-970; 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent.
Synonyms	DAM
Reference Data

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