Cd80 Rat Monoclonal Antibody [Clone ID: RM80]

Specifications

Product Data
Clone Name	RM80
Applications	FC, IHC
Recommend Dilution	Flow cytometry: 1/10 - 1/25, use 10 µl of the suggested working dilution to label 1x10e6 cells in 100 µl. Immunohistochemistry on frozen sections. Functional assays.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	BCL1 cells expressing CD80
Specificity	This antibody recognises CD80 (B7-1), a 60kD cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells. Expression may be significantly increased upon B lymphocytes by LPS and by IL-4.
Isotype	IgG2a
Formulation	Phosphate buffered saline, pH 7.4 containing 0.09% sodium azide. State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD80 antigen
Database Link	Entrez Gene 12519 Mouse UniProt ID Q00609
Background	CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.
Synonyms	CD28LG, CD28LG1, LAB7, BB1, B7.1, B7-1
Reference Data

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