IL12B Mouse Monoclonal Antibody [Clone ID: CC326]

Specifications

Product Data
Clone Name	CC326
Applications	ELISA, FC, FN
Recommend Dilution	ELISA: 1/100-1/1000. Flow Cytometry: Use 10 µl of 1/1000 diluted antibody to label 10e6 cells of 100 µl. Membrane permeabilisation is required. Functional assays: 1/100.
Reactivity	Bovine, Human, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Bovine IL-12. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Specificity	This antibody recognizes Interleukin-12. It has been shown to block the biological activity of IL-12.
Isotype	IgG2b
Formulation	PBS, pH 7.4 State: Azide Free State: Liquid purified Ig fraction from Tissue Culture Supernatant Stabilizer: None Preservative: None
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 281857 Bovine UniProt ID P46282
Background	Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10; homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.
Synonyms	Interleukin-12B, IL-12B, IL-12 subunit p40, CLMF p40, NKSF2
Reference Data

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