CD52 Rat Monoclonal Antibody [Clone ID: YTH66.9HL]

Specifications

Product Data
Clone Name	YTH66.9HL
Applications	CT, FC, IHC, WB
Recommend Dilution	Immunohistochemistry on frozen sections: 1:20. Western blot. Flow cytometry: Use 10 µl of 1:20 diluted antibody to label 10e6 cells in 100 µl. Cytotoxic assays.
Reactivity	Human, Monkey
Host	Rat
Clonality	Monoclonal
Immunogen	Human T lymphocytes
Specificity	This antibody reacts with the human CD52 antigen, also known as CAMPATH-1. The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kDa.
Isotype	IgM
Formulation	Phosphate buffered saline 0.09% Sodium Azide State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Ammonium sulphate precipitation
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Gene Name	CD52 molecule
Database Link	Entrez Gene 1043 Human UniProt ID P31358
Background	CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is epressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
Synonyms	HE5, CDw52
Reference Data

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